Localization of Urokinase Type Plaminogen Activator Receptor on Tumor Cells by Tmmunohistochemistry

Park, Hye Won; Kook, Yoon Hoh; Choi, Sung Bae; Cha, ng Yong

J Korean Cancer Assoc > Volume 28(1); 1996 > Article

Original Article

Journal of the Korean Cancer Association 1996;28(1): 159-168.

유로키나제형 플라즈미노겐 액티베이터 ( uPA ) 수용체의 암세포상의 면역화학적 염색상

박혜원, 국윤호, 최성배, 차창용

Localization of Urokinase Type Plaminogen Activator Receptor on Tumor Cells by Tmmunohistochemistry

Hye Won Park, Yoon Hoh Kook, Sung Bae Choi, ng Yong Cha

ABSTRACT

Urokinase type plasminogen activator(uPA) plays important role in invasion and metastasis of cancer. Its actions are activated and amplified by a specific cell-surface receptor (urokinase type plasminogen activator receptor: uPAR). The present study was undertaken to determine the importance of the uPAR for migration and invasion of cancer cells through immunohistochemical localization of uPAR. HEp3 cells were cultured in monolayer and suspended, then they were stained by immunofluorescence, APAAP(alkaline phosphatase anti-alkaline phosphatase) and immunoperoxidase method. The receptor(uPAR) was found to be located at cellular contact sites. In suspension uPAR staining of HEp3 cells showed focal distribution. In vivo model of invasion, the uPAR was localized on leading edge of tumor of cholioallatoic membrane(CAM) inoculated with HEp3 cells. This suggests that uPAR might play crucial role in migration and invasion of cancer cells through mediating proteolysis at cellular contact sites especially in leading edge of the tumor.

Key words: Urokinase type plasminogen activator receptor, Cancer, Cell migration, Invasion, Immunohistochemistry