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J Korean Cancer Assoc > Volume 26(2); 1994 > Article
Journal of the Korean Cancer Association 1994;26(2): 231-236.
3 주령 흰쥐에서 디에칠니트로사민 투여에 의한 GST - P+ foci 의 유도 : GST - P+ foci 를 이용한 새로운 중기 간 발암 실험법의 시도
장자준, 이윤실, 이미숙, 김태환
Development of GST - P+ Foci in 3 Week Old Rast Inducted by Diethylnitrosamine : Preliminary data for liver foci model
Ja June Jang, Yun Sil Lee, Mi Sook Lee, Tae Hwan Kim
A new medium-term in vivo hepatocarcinogenesis model was tried using GST-P hepatic foci. The treatment protocol was the i.p. injection of 50 mg/kg body weight of diethylnitrosamine(DEN) ance, twice or three times per week with or without 0.05% phenobarbital(PB) in 3 week female Sprague-Dawley rats or male Fischer 344 rats. After 9 weeks, the rats were sacri- ficed and their livers were examined for immunohistochemically detectable GST-P' foci. DEN treatment in 3 week old rats without PB was enough to induce GST-P foci. This protocol is very simple and convenient compared to other liver foci model using newborn rats or com- bined partial hepatectomy. And the yield of GST-P' foci treated with DEN and PB was almost equal as other models. Therefore, this simple hepatic foci model may be useful for screening of carcinogenic or anticarcinogenic agents. For the validity of new model, more extensive studies will be necessary.
Key words: GST-P' foci, Diethylnitrosamine, Rat, Liver model
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