A new medium-term in vivo hepatocarcinogenesis model was tried using GST-P hepatic foci. The treatment protocol was the i.p. injection of 50 mg/kg body weight of diethylnitrosamine(DEN) ance, twice or three times per week with or without 0.05% phenobarbital(PB) in 3 week female Sprague-Dawley rats or male Fischer 344 rats. After 9 weeks, the rats were sacri- ficed and their livers were examined for immunohistochemically detectable GST-P' foci. DEN treatment in 3 week old rats without PB was enough to induce GST-P foci. This protocol is very simple and convenient compared to other liver foci model using newborn rats or com- bined partial hepatectomy. And the yield of GST-P' foci treated with DEN and PB was almost equal as other models. Therefore, this simple hepatic foci model may be useful for screening of carcinogenic or anticarcinogenic agents. For the validity of new model, more extensive studies will be necessary.
PDF Links
Full text via DOI 
Download Citation 
