Cancer Research and Treatment

Original Articles

Gastrointestinal cancer

Anti–PD-L1 Antibody and/or 17β-Estradiol Treatment Induces Changes in the Gut Microbiome in MC38 Colon Tumor Model: Chin-Hee Song, Nayoung Kim, Ryoung Hee Nam, Soo In Choi, Jae Young Jang, Jina Choi, Ha-Na Lee; Cancer Res Treat. 2023;55(3):894-909. Published online January 9, 2023; DOI: https://doi.org/10.4143/crt.2022.1427

Purpose
17β-Estradiol (E2) supplementation suppresses MC38 tumor growth by downregulating the expression of programmed death-ligand 1 (PD-L1). This study aims to figure out the gut microbiota that respond to anti–PD-L1 and/or estrogen treatment in MC38 colon cancer model.
Materials and Methods
A syngeneic colon tumor model was developed by injection of MC38 cells into C57BL/6 background male and female mice. Three days before MC38 cells injection, E2 was supplemented to male mice daily for 1 week. Male and female mice with MC38 tumors (50-100 mm3) were injected with anti–PD-L1 antibody. Fresh feces were collected 26 days after injection of MC38 cells and 16S rRNA metagenomics sequencing of DNA extracted from feces was used to assess gut microbial composition.
Results
At the taxonomic family level, Muribaculaceae was enriched only in the MC38 male control group. In male mice, linear discriminant analysis effect size analysis at the species level revealed that the four microorganisms were commonly regulated in single and combination treatment with anti–PD-L1 and/or E2; a decrease in PAC001068_g_uc and PAC001070_s (family Muribaculaceae) and increase in PAC001716_s and PAC001785_s (family Ruminococcaceae). Interestingly, in the anti–PD-L1 plus E2 group, a decrease in opportunistic pathogens (Enterobacteriaceae group) and an increase in commensal bacteria (Lactobacillus murinus group and Parabacteroides goldsteinii) were observed. Furthermore, the abundance of Parabacteroides goldsteinii was increased in both males and females in the anti–PD-L1 group.
Conclusion
Our results suggest that gut microbial changes induced by the pretreatment of estrogen before anti–PD-L1 might contribute to treatment of MC38 colon cancer.

Citations

Citations to this article as recorded by

Gut microbiome–driven colorectal cancer via immune, metabolic, neural, and endocrine axes reprogramming
Jhommara Bautista, Mónica Lamas-Maceiras, Camila Hidalgo-Tinoco, Antonio Guerra-Guerrero, Anapaula Betancourt-Velarde, Andrés López-Cortés
npj Biofilms and Microbiomes.2026;[Epub] CrossRef
Gastrointestinal cancers in women: The role of sex hormones
Amit Yadav, Usha Dutta
Indian Journal of Gastroenterology.2026; 45(2): 211. CrossRef
Wuda granules target estrogen receptors and modulate gut microbiota to alleviate postoperative ileus: A multi-omics perspective
Tao Wang, Yi-Hua Xu, Yang-Hui Ou, Wen-Jun Xiong, Li-Jie Luo, Jin Li, Yao-Hui Peng, Yan Chen, Hai-Ping Zeng, Yang Yu, Hai-Peng Tang, Fei Wang, Hong-Liang Yao, Wei Wang
World Journal of Gastroenterology.2026;[Epub] CrossRef
Effects of letrozole supplementation on growth performance, plasma hormones, and plasma metabolites in weaned female lambs of Turpan black sheep
Tingting Li, Hui Chen, Jiaqi Liu, Hao Lu, Tingting Lu, Reyilaguli Reyimu, Xihu Wang, Jianjun Zhang, Shijie Li, Xiaojun Liu, Rui Xiao, Guodong Zhao, Kailun Yang
Frontiers in Veterinary Science.2026;[Epub] CrossRef
Polygonatum kingianum polysaccharides ameliorate cognitive impairments in alcohol-exposed obese mice in a sex-specific manner by regulating the gut microbiome and metabolome
Jiayao Lv, Yizhe Qu, Qixin Yan, Ken Cheng, Qingyang Wu, Jiayi Zhang, Xinting Duan, Shanshan Han, Honglei Tian, Lin Shi
Food Research International.2026; 242: 119827. CrossRef
Association between serum short-chain fatty acid levels and the risk of all-cause and cardiovascular disease mortality in Chinese patients undergoing maintenance hemodialysis: a retrospective cohort study
Xiu-Nan Zhao, Shu-Xin Liu, Shuang Zhang, Zhen-Zhen Wang, Zi Lin, Xue-Lian Jian, Cui Dong, Yi-Nan Zhang
Clinical Kidney Journal.2025;[Epub] CrossRef
Parabacteroides as a promising target for disease intervention: current stage and pending issues
Jing Liu, Hui Qiu, Jiamin Zhao, Nan Shao, Chao Chen, Zhixu He, Xu Zhao, Juanjuan Zhao, Ya Zhou, Lin Xu
npj Biofilms and Microbiomes.2025;[Epub] CrossRef
Therapeutic potential of the gut commensal bacterium Parabacteroides goldsteinii in human health and disease treatment
Ziyun Li, Li Zhang, Zhenxia Wan, Huijuan Liu, Ting Zhang, Yan Li
Frontiers in Microbiology.2025;[Epub] CrossRef
Mechanism of the AMPK/SIRT1 pathway in gut dysbiosis–mediated postoperative cognitive dysfunction in aged mice
Fu Xu, Yang Yue, Defeng Sun
International Journal of Neuropsychopharmacology.2025;[Epub] CrossRef
Unveiling the interplay between microbiota and PD1/PD-L1 axis in tumor immunity and immunotherapy
Qiguang Lu, Jiasheng Wu, Xiaoyan Yu, Juanjuan Qian, Zhengwei Song
Frontiers in Immunology.2025;[Epub] CrossRef
Distribution and roles of Ligilactobacillus murinus in hosts
Zhou Chuandong, Jicong Hu, Jiawen Li, Yuting Wu, Chan Wu, Guanxi Lai, Han Shen, Fenglin Wu, Changli Tao, Song Liu, Wenfeng Zhang, Hongwei Shao
Microbiological Research.2024; 282: 127648. CrossRef
Sex differences in colorectal cancer: with a focus on sex hormone–gut microbiome axis
Zihong Wu, Yuqing Huang, Renyi Zhang, Chuan Zheng, Fengming You, Min Wang, Chong Xiao, Xueke Li
Cell Communication and Signaling.2024;[Epub] CrossRef
17β-estradiol in colorectal cancer: friend or foe?
Zihong Wu, Chong Xiao, Jiamei Wang, Min Zhou, Fengming You, Xueke Li
Cell Communication and Signaling.2024;[Epub] CrossRef
Sexual dimorphism of gut microbiota in colorectal cancer
Zihong Wu, Ziming Wang, Jiamei Wang, Chong Xiao, Fengming You, Xueke Li
Chinese Science Bulletin.2024; 69(35): 5142. CrossRef
Direct and indirect effects of estrogens, androgens and intestinal microbiota on colorectal cancer
Zihong Wu, Yi Sun, Wenbo Huang, Zhenzhen Jin, Fengming You, Xueke Li, Chong Xiao
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Targeting metabolic pathways: a novel therapeutic direction for type 2 diabetes
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Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef

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Sarcoma

Clinicopathologic Characteristics and Clinical Outcome of Localized Liposarcoma: A Single-Center Experience over 25 Years and Evaluation of PD-L1 Expression: Heejung Chae, Jeong Eun Kim, Wanlim Kim, Jong-Seok Lee, Si Yeol Song, Min Hee Lee, Hye Won Chung, Kyung-Ja Cho, Joon Seon Song, Jin-Hee Ahn; Cancer Res Treat. 2022;54(2):579-589. Published online July 6, 2021; DOI: https://doi.org/10.4143/crt.2021.496

Abstract PDF Supplementary Material PubReader ePub

Purpose
For liposarcoma (LPS), clinical course and proper treatment strategies have not been well-established. Recently, immune-checkpoint inhibitors have shown potential efficacy in LPS. We aimed to describe the clinical course of LPS and evaluate the clinical impact of programmed death-ligand 1 (PD-L1).
Materials and Methods
We reviewed all consecutive patients (n=332) who underwent curative-intent surgery for localized LPS at Asan Medical Center between 1989 and 2017. PD-L1 testing was performed in well-differentiated and dedifferentiated LPS.
Results
The median age was 56 years with males comprising 60.8%. Abdomen-pelvis (47.6%) and well-differentiated (37.7%) were the most frequent primary site and histologic subtype, respectively. During a median follow-up of 81.2 months, recurrence was observed in 135 (40.7%), and 86.7% (117/135) were loco-regional. Well-differentiated subtype (hazard ratio [HR], 0.38), abdomen-pelvis origin (HR, 2.43), tumor size larger than 5 cm (HR, 1.83), positive resection margin (HR, 2.58), and postoperative radiotherapy (HR, 0.36) were significantly related with recurrence-free survival as well as visceral involvement (HR, 1.84) and multifocality (HR, 3.79) in abdomen-pelvis LPS. PD-L1 was positive in 31.5% (23/73) and 51.3% (39/76) of well-differentiated and dedifferentiated LPS, respectively, but had no impact on survival outcomes.
Conclusion
Clinical course of LPS was heterogeneous according to histology and anatomic location. Clear resection margin was important to lower recurrence and postoperative radiotherapy might have additional benefit. A decent portion of well-differentiated and dedifferentiated LPS were positive for PD-L1, but its prognostic role was unclear. Further research is needed to determine clinical implications of PD-L1, especially for advanced-stage LPS with unmet needs for effective systemic treatment.

Citations

Citations to this article as recorded by

Recurrent Intrathoracic Liposarcoma: A Case Report and a Comprehensive Literature Review of a Rare Clinical Entity
Vasileios Leivaditis , Manfred Dahm , Athanasios Papatriantafyllou, Hans-Georg Keul, Lydia Kohl, Hans-Joachim Schäfers
Cureus.2024;[Epub] CrossRef
Clinicopathological Features of Intrathoracic Liposarcoma—A Systematic Review with an Illustrative Case
Kajetan Kiełbowski, Nikola Ruszel, Seweryn Adam Skrzyniarz, Małgorzata Edyta Wojtyś, Rafał Becht, Konrad Ptaszyński, Darko Gajić, Janusz Wójcik
Journal of Clinical Medicine.2022; 11(24): 7353. CrossRef

8,900 View
124 Download
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2 Crossref

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