Cancer Research and Treatment

Original Articles

General

Establishment of Patient-Derived Organoids Using Ascitic or Pleural Fluid from Cancer Patients: Wonyoung Choi, Yun-Hee Kim, Sang Myung Woo, Yebeen Yu, Mi Rim Lee, Woo Jin Lee, Jung Won Chun, Sung Hoon Sim, Heejung Chae, Hyoeun Shim, Keun Seok Lee, Sun-Young Kong; Cancer Res Treat. 2023;55(4):1077-1086. Published online June 12, 2023; DOI: https://doi.org/10.4143/crt.2022.1630

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Purpose
Patient-derived tumor cells can be a powerful resource for studying pathophysiological mechanisms and developing robust strategies for precision medicine. However, establishing organoids from patient-derived cells is challenging because of limited access to tissue specimens. Therefore, we aimed to establish organoids from malignant ascites and pleural effusions.
Materials and Methods
Ascitic or pleural fluid from pancreatic, gastric, and breast cancer patients was collected and concentrated to culture tumor cells ex vivo. Organoids were considered to be successfully cultured when maintained for five or more passages. Immunohistochemical staining was performed to compare the molecular features, and drug sensitivity was assayed to analyze the clinical responses of original patients.
Results
We collected 70 fluid samples from 58 patients (pancreatic cancer, n=39; gastric cancer, n=21; and breast cancer, n=10). The overall success rate was 40%; however, it differed with types of malignancy, with pancreatic, gastric, and breast cancers showing 48.7%, 33.3%, and 20%, respectively. Cytopathological results significantly differed between successful and failed cases (p=0.014). Immunohistochemical staining of breast cancer organoids showed molecular features identical to those of tumor tissues. In drug sensitivity assays, pancreatic cancer organoids recapitulated the clinical responses of the original patients.
Conclusion
Tumor organoids established from malignant ascites or pleural effusion of pancreatic, gastric, and breast cancers reflect the molecular characteristics and drug sensitivity profiles. Our organoid platform could be used as a testbed for patients with pleural and peritoneal metastases to guide precision oncology and drug discovery.

Citations

Citations to this article as recorded by

PRMT1 promotes pancreatic cancer development and resistance to chemotherapy
Bomin Ku, David Eisenbarth, Seonguk Baek, Tae-Keun Jeong, Ju-Gyeong Kang, Daehee Hwang, Myung-Giun Noh, Chan Choi, Sungwoo Choi, Taejun Seol, Hail Kim, Yun-Hee Kim, Sang Myung Woo, Sun-Young Kong, Dae-Sik Lim
Cell Reports Medicine.2024; 5(3): 101461. CrossRef
Establishment and Advancement of Pancreatic Organoids
Dong Hyeon Lee
Keimyung Medical Journal.2024; 43(1): 3. CrossRef
Organoid as a promising tool for primary liver cancer research: a comprehensive review
Xuekai Hu, Jiayun Wei, Pinyan Liu, Qiuxia Zheng, Yue Zhang, Qichen Zhang, Jia Yao, Jingman Ni
Cell & Bioscience.2024;[Epub] CrossRef
The use of organoids in creating immune microenvironments and treating gynecological tumors
Ling-Feng Zhou, Hui-Yan Liao, Yang Han, Yang Zhao
Journal of Translational Medicine.2024;[Epub] CrossRef
Organoid: Bridging the gap between basic research and clinical practice
Guihu Weng, Jinxin Tao, Yueze Liu, Jiangdong Qiu, Dan Su, Ruobing Wang, Wenhao Luo, Taiping Zhang
Cancer Letters.2023; 572: 216353. CrossRef

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Clinical Significance of Pleural Attachment and Indentation of Subsolid Nodule Lung Cancer: Hyung-Jun Kim, Jun Yeun Cho, Yeon Joo Lee, Jong Sun Park, Young-Jae Cho, Ho Il Yoon, Jin-Haeng Chung, Sukki Cho, Kwhanmien Kim, Kyung Won Lee, Jae Ho Lee, Choon-Taek Lee; Cancer Res Treat. 2019;51(4):1540-1548. Published online March 25, 2019; DOI: https://doi.org/10.4143/crt.2019.057

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Purpose
Lung cancers presenting as subsolid nodule commonly have peripheral location, making the cancer-pleura relationship noteworthy. We aimed to evaluate the effect of pleural attachment and/or indentation on visceral pleural invasion (VPI) and recurrence-free survival.
Materials and Methods
Patients who underwent curative resection of lung cancer as subsolid nodules from April 2007 to January 2016 were retrospectively evaluated. They were divided into four groups according to their relationship with the pleura. Clinical, radiographical, and pathological findings were analyzed.
Results
Among 404 patients with malignant subsolid nodule, 120 (29.7%) had neither pleural attachment nor indentation, 26 (6.4%) had attachment only, 117 (29.0%) had indentation only, and 141 (34.9%) had both. VPI was observed in nodules of 36 patients (8.9%), but absent in nonsolid nodules and in those without pleural attachment and/or indentation. Compared to subsolid nodules with concurrent pleural attachment and indentation, those with attachment only (odds ratio, 0.12; 95% confidence interval [CI], 0.02 to 0.98) and indentation only (odds ratio, 0.10; 95% CI, 0.03 to 0.31) revealed lower odds of VPI. On subgroup analysis, the size of the solid portion was associated with VPI among those with pleural attachment and indentation (p=0.021). Such high-risk features for VPI were associated with earlier lung cancer recurrence (adjusted hazard ratio, 3.31; 95% CI, 1.58 to 6.91).
Conclusion
Concurrent pleural attachment and indentation are risk factors for VPI, and the odds increase with larger solid portion in subsolid nodules. Considering the risk of recurrence, early surgical resection could be encouraged in these patients.

Citations

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A CT-based deep learning model: visceral pleural invasion and survival prediction in clinical stage IA lung adenocarcinoma
Xiaofeng Lin, Kunfeng Liu, Kunwei Li, Xiaojuan Chen, Biyun Chen, Sheng Li, Huai Chen, Li Li
iScience.2024; 27(1): 108712. CrossRef
CT-Based Intratumoral and Peritumoral Radiomics Nomograms for the Preoperative Prediction of Spread Through Air Spaces in Clinical Stage IA Non-small Cell Lung Cancer
Yun Wang, Deng Lyu, Lei Hu, Junhong Wu, Shaofeng Duan, Taohu Zhou, Wenting Tu, Yi Xiao, Li Fan, Shiyuan Liu
Journal of Imaging Informatics in Medicine.2024; 37(2): 520. CrossRef
Prognostic Analysis of Stage I Non-Small Cell Lung Cancer Abutting Adjacent Structures on Preoperative Computed Tomography
Soohwan Choi, Sun Kyun Ro, Seok Whan Moon
Journal of Chest Surgery.2024; 57(2): 136. CrossRef
Nomogram using intratumoral and peritumoral radiomics for the preoperative prediction of visceral pleural invasion in clinical stage IA lung adenocarcinoma
Yun Wang, Deng Lyu, Su Hu, Yanqing Ma, Shaofeng Duan, Yayuan Geng, Taohu Zhou, Wenting Tu, Yi Xiao, Li Fan, Shiyuan Liu
Journal of Cardiothoracic Surgery.2024;[Epub] CrossRef
Nomogram for predicting invasive lung adenocarcinoma in small solitary pulmonary nodules
Mengchao Xue, Rongyang Li, Junjie Liu, Ming Lu, Zhenyi Li, Huiying Zhang, Hui Tian
Frontiers in Oncology.2024;[Epub] CrossRef
Analysis of postoperative recurrence-free survival in non–small cell lung cancer patients based on consensus clustering
Q. Tian, S.-Y. Zhou, Y.-H. Qin, Y.-Y. Wu, C. Qin, H. Zhou, J. Shi, S.-F. Duan, F. Feng
Clinical Radiology.2024; 79(10): e1214. CrossRef
Predicting bone metastasis-free survival in non-small cell lung cancer from preoperative CT via deep learning
Jia Guo, Jianguo Miao, Weikai Sun, Yanlei Li, Pei Nie, Wenjian Xu
npj Precision Oncology.2024;[Epub] CrossRef
Tumour–pleura relationship on CT is a risk factor for occult lymph node metastasis in peripheral clinical stage IA solid adenocarcinoma
Chengzhou Zhang, Liping Wang, Xiaoting Cai, Mengfei Li, Dandan Sun, Ping Wang
European Radiology.2023; 33(5): 3083. CrossRef
Analysis of the relevance between computed tomography characterization and pathology of pulmonary ground-glass nodules with different pathology types
Zhang Youguo, Wang Chengye, Cheng Xiaofei, Zhang Xuefei, Liu Changhong
Turkish Journal of Thoracic and Cardiovascular Surgery.2023; 31(1): 95. CrossRef
Risk factors for loss of pulmonary function after wedge resection for peripheral ground-glass opacity dominant lung cancer
Tomohiro Miyoshi, Hiroyuki Ito, Masashi Wakabayashi, Tadayoshi Hashimoto, Yuta Sekino, Kenji Suzuki, Masahiro Tsuboi, Yasumitsu Moriya, Ichiro Yoshino, Tetsuya Isaka, Aritoshi Hattori, Takahiro Mimae, Mitsuhiro Isaka, Tomohiro Maniwa, Makoto Endo, Hiroshi
European Journal of Cardio-Thoracic Surgery.2023;[Epub] CrossRef
Volumetric analysis of intravoxel incoherent motion diffusion-weighted imaging in preoperative assessment of non-small cell lung cancer
Jianqin Jiang, Yigang Fu, Lili Zhang, Jia Liu, Xiaowen Gu, Weiwei Shao, Lei Cui, Gaofeng Xu
Japanese Journal of Radiology.2022; 40(9): 903. CrossRef
A Nomogram Combined Radiomics and Clinical Features as Imaging Biomarkers for Prediction of Visceral Pleural Invasion in Lung Adenocarcinoma
Xinyi Zha, Yuanqing Liu, Xiaoxia Ping, Jiayi Bao, Qian Wu, Su Hu, Chunhong Hu
Frontiers in Oncology.2022;[Epub] CrossRef
Radiologists with and without deep learning–based computer-aided diagnosis: comparison of performance and interobserver agreement for characterizing and diagnosing pulmonary nodules/masses
Tomohiro Wataya, Masahiro Yanagawa, Mitsuko Tsubamoto, Tomoharu Sato, Daiki Nishigaki, Kosuke Kita, Kazuki Yamagata, Yuki Suzuki, Akinori Hata, Shoji Kido, Noriyuki Tomiyama
European Radiology.2022; 33(1): 348. CrossRef
The value of CT radiomics features to predict visceral pleural invasion in ≤3 cm peripheral type early non-small cell lung cancer
Shu-Hua Wei, Jin-Mei Zhang, Bin Shi, Fei Gao, Zhao-Xuan Zhang, Li-Ting Qian
Journal of X-Ray Science and Technology.2022; 30(6): 1115. CrossRef
Pathological components and CT imaging analysis of the area adjacent pleura within the pure ground-glass nodules with pleural deformation in invasive lung adenocarcinoma
Yining Jiang, Ziqi Xiong, Wenjing Zhao, Di Tian, Qiuping Zhang, Zhiyong Li
BMC Cancer.2022;[Epub] CrossRef
Development and validation of a deep learning signature for predicting lymph node metastasis in lung adenocarcinoma: comparison with radiomics signature and clinical-semantic model
Xiaoling Ma, Liming Xia, Jun Chen, Weijia Wan, Wen Zhou
European Radiology.2022; 33(3): 1949. CrossRef
Lung-RADS Version 1.1: Challenges and a Look Ahead, From the AJR Special Series on Radiology Reporting and Data Systems
Lydia Chelala, Rydhwana Hossain, Ella A. Kazerooni, Jared D. Christensen, Debra S. Dyer, Charles S. White
American Journal of Roentgenology.2021; 216(6): 1411. CrossRef
Predicting pathological lymph node status in clinical stage IA peripheral lung adenocarcinoma
Keiju Aokage, Kenji Suzuki, Masashi Wakabayashi, Tomonori Mizutani, Aritoshi Hattori, Haruhiko Fukuda, Shun-Ichi Watanabe
European Journal of Cardio-Thoracic Surgery.2021; 60(1): 64. CrossRef
Discriminating Small-Sized (2 cm or Less), Noncalcified, Solitary Pulmonary Tuberculoma and Solid Lung Adenocarcinoma in Tuberculosis-Endemic Areas
Jingping Zhang, Tingting Han, Jialiang Ren, Chenwang Jin, Ming Zhang, Youmin Guo
Diagnostics.2021; 11(6): 930. CrossRef
Association of postoperative recurrence with radiological and clinicopathological features in patients with stage IA–IIA lung adenocarcinoma
Yanyan Zhang, Fengnian Zhao, Minghao Wu, Yunqing Zhao, Ying Liu, Qian Li, Guiming Zhou, Zhaoxiang Ye
European Journal of Radiology.2021; 141: 109802. CrossRef
Characterization of Newly Detected Costal Pleura–attached Noncalcified Nodules at Annual Low-Dose CT Screenings
Yeqing Zhu, Rowena Yip, Nan You, Qiang Cai, Claudia I. Henschke, David F. Yankelevitz, Claudia I. Henschke, David F. Yankelevitz, Rowena Yip, Dongming Xu, Mary Salvatore, Raja Flores, Andrea Wolf, David S. Mendelson, Dorothy I. McCauley, Mildred Chen, Dan
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Clinicopathological and computed tomographic features associated with occult lymph node metastasis in patients with peripheral solid non-small cell lung cancer
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European Journal of Radiology.2021; 144: 109981. CrossRef
Surgical Extent for Ground Glass Nodules
Suk Ki Cho
Journal of Chest Surgery.2021; 54(5): 338. CrossRef
CT-guided microcoil localization for pulmonary nodules before VATS: a retrospective evaluation of risk factors for pleural marking failure
Yanyan Xu, Lingchuan Ma, Hongliang Sun, Zhenguo Huang, Zhenrong Zhang, Fei Xiao, Qianli Ma, Chuandong Li, Xiaomeng Zhang, Sheng Xie
European Radiology.2020; 30(10): 5674. CrossRef
Management of Nodules Attached to the Costal Pleura at Low-Dose CT Screening for Lung Cancer
Yeqing Zhu, Rowena Yip, Nan You, Claudia I. Henschke, David F. Yankelevitz
Radiology.2020; 297(3): 710. CrossRef

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EGFR Mutation Status in Lung Adenocarcinoma-Associated Malignant Pleural Effusion and Efficacy of EGFR Tyrosine Kinase Inhibitors: Jiyoul Yang, Ok-Jun Lee, Seung-Myoung Son, Chang Gok Woo, Yusook Jeong, Yaewon Yang, Jihyun Kwon, Ki Hyeong Lee, Hye Sook Han; Cancer Res Treat. 2018;50(3):908-916. Published online September 19, 2017; DOI: https://doi.org/10.4143/crt.2017.378

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Purpose
Malignant pleural effusions (MPEs) are often observed in lung cancer, particularly adenocarcinoma. The aim of this study was to investigate epidermal growth factor receptor (EGFR) mutation status in lung adenocarcinoma-associated MPEs (LA-MPEs) and its correlation with efficacy of EGFR tyrosine kinase inhibitor (TKI) therapy.
Materials and Methods
Samples comprised 40 cell blocks of pathologically-confirmed LA-MPEs collected before the start of EGFR TKI therapy. EGFR mutation status was re-evaluated by peptide nucleic acid clamping and the clinical outcomes of EGFR TKI‒treated patients were analyzed retrospectively.
Results
EGFR mutations were detected in 72.5% of LA-MPE cell blocks (29/40). The median progression-free survival for patients with EGFR mutations in LA-MPEs was better than that for patients with wild-type EGFR (7.33 months vs. 2.07 months; hazard ratio, 0.486; 95% confidence interval, 0.206 to 1.144; p=0.032). The objective response rate (ORR) of 26 patients with EGFR mutations in LA-MPEs among the 36 patients with measurable lesions was 80.8%, while the ORR of the 10 patients with wild-type EGFR in LA-MPEs was 10% (p < 0.001). Among the 26 patients with EGFR mutations in LA-MPEs, the ORR of target lesions and LA-MPEs were 88.5% and 61.5%, respectively (p=0.026).
Conclusion
EGFR mutation status in cell blocks of LA-MPEs confirmed by pathologic diagnosis is highly predictive of EGFR TKI efficacy. For patients with EGFR mutations in LA-MPEs, the response to EGFR TKIs seems to be worse for pleural effusions than for solid tumors.

Citations

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Cancer‐associated fibroblast‐derived colony‐stimulating factor 2 confers acquired osimertinib resistance in lung adenocarcinoma via promoting ribosome biosynthesis
Yutang Huang, Xiaoqing Wang, Chunjie Wen, Jingchan Wang, Honghao Zhou, Lanxiang Wu
MedComm.2024;[Epub] CrossRef
Effect of chemotherapy, immunotherapy, and targeted therapies on removal of indwelling pleural catheters in non-small cell lung cancer patients with malignant pleural effusions
Melissa Wang, Kaitlin Sparrow, Chrystal Chan, Ashley Gillson, Daniel Stollery, Pen Li
Respiratory Medicine.2023; 206: 107093. CrossRef
Malignant pleural effusion diagnosis and therapy
Liangliang Yang, Yue Wang
Open Life Sciences.2023;[Epub] CrossRef
Failure pattern and radiotherapy exploration in malignant pleural effusion non-small cell lung cancer treated with targeted therapy
Qingsong Li, Cheng Hu, Shengfa Su, Zhu Ma, Yichao Geng, Yinxiang Hu, Huiqin Li, Bing Lu
Frontiers in Oncology.2023;[Epub] CrossRef
Impact of thoracic tumor radiotherapy on survival in non‐small‐cell lung cancer with malignant pleural effusion treated with targeted therapy: Propensity score matching study
Qingsong Li, Cheng Hu, Shengfa Su, Zhu Ma, Yichao Geng, Yinxiang Hu, Haijie Jin, Huiqin Li, Bing Lu
Cancer Medicine.2023; 12(14): 14949. CrossRef
EGFR Mutation is a Prognostic Factor in Lung Cancer Patients with Pleural Dissemination Detected During or After Surgery
Toshiya Fujiwara, Kazuhiko Shien, Motoki Matsuura, Junichi Soh, Hiromasa Yamamoto, Soshi Takao, Yuho Maki, Tsuyoshi Ueno, Ryujiro Sugimoto, Ken Suzawa, Mikio Okazaki, Hiroyuki Tao, Makio Hayama, Masafumi Kataoka, Yoshifumi Sano, Hidetoshi Inokawa, Motohir
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Scientific Reports.2023;[Epub] CrossRef
Establishment of prognostic nomograms for predicting the progression free survival of EGFR‐sensitizing mutation, advanced lung cancer patients treated with EGFR‐TKIs
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Non-Small Cell Lung Cancer with Malignant Pleural Effusion May Require Primary Tumor Radiotherapy in Addition to Drug Treatment
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Risk Factors for and Time to Recurrence of Symptomatic Malignant Pleural Effusion in Patients With Metastatic Non-Small Cell Lung Cancer with EGFR or ALK Mutations
Audra J. Schwalk, David E. Ost, Sahara N. Saltijeral, Henriette De La Garza, Roberto F. Casal, Carlos A. Jimenez, Georgie A. Eapen, Jeff Lewis, Waree Rinsurongkawong, Vadeerat Rinsurongkawong, Jack Lee, Yasir Elamin, Jianjun Zhang, Jack A. Roth, Stephen S
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Tetsuo Tani, Ichiro Nakachi, Shinnosuke Ikemura, Shigenari Nukaga, Keiko Ohgino, Aoi Kuroda, Hideki Terai, Keita Masuzawa, Taro Shinozaki, Kota Ishioka, Yohei Funatsu, Hidefumi Koh, Koichi Fukunaga, Kenzo Soejima
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Daniel Pinto, Fernando Schmitt
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Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer
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Sara Raimundo, Ana Isabel Loureiro, Fortunato Vieira, Ana Fernandes
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Pranav Murthy, Chigozirim N. Ekeke, Kira L. Russell, Samuel C. Butler, Yue Wang, James D. Luketich, Adam C. Soloff, Rajeev Dhupar, Michael T. Lotze
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Momin T. Siddiqui, Fernando Schmitt, Andrew Churg
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Diagnostic Value of Circulating Extracellular miR-134, miR-185, and miR-22 Levels in Lung Adenocarcinoma-Associated Malignant Pleural Effusion: Yoon Mi Shin, Jieun Yun, Ok-Jun Lee, Hye-Suk Han, Sung-Nam Lim, Jin Young An, Ki Hyeong Lee, Ki Man Lee, Kang Hyeon Choe; Cancer Res Treat. 2014;46(2):178-185. Published online April 15, 2014; DOI: https://doi.org/10.4143/crt.2014.46.2.178

Abstract PDF PubReader ePub

Purpose

The accurate and timely diagnosis of malignant pleural effusion (MPE) in lung cancer patients is important because MPE has a poor prognosis and is classified as stage IV disease. Molecular biomarkers for pleural effusion, such as circulating extracellular microRNAs (miRNAs) isolated from pleural fluid, may help in the diagnosis of MPE. The present study examined whether miRNAs that are deregulated in lung cancer (miR-134, miR-185, and miR-22) can serve as diagnostic markers for lung adenocarcinoma-associated MPE (LA-MPE).

Materials and Methods

Real-time reverse transcription quantitative polymerase chain reaction was used to measure the expression of the three miRNAs in samples from 87 patients with pleural effusion comprising 45 LA-MPEs and 42 benign pleural effusions (BPEs). The area under the receiver operating characteristic curve (AUC) was then used to evaluate the diagnostic performance of each of the three miRNAs and compare it with that of the common tumor marker, carcinoembryonic antigen (CEA).

Results

The expression of all three miRNAs was significantly lower in LA-MPE than in BPE (p <0.001). The AUCs for miR-134, miR-185, miR-22, and CEA were 0.721, 0.882, 0.832, and 0.898, respectively. Combining CEA with the three miRNAs increased the diagnostic performance, yielding an AUC of 0.942 (95% confidence interval, 0.864 to 0.982), with a sensitivity of 91.9% and a specificity of 92.5%.

Conclusion

The present study suggests that the expression levels of circulating extracellular miR-134, miR-185, and miR-22 in patients with pleural effusion may have diagnostic value when differentiating between LA-MPE and BPE.

Citations

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Molecular mechanisms and clinical applications of miR-22 in regulating malignant progression in human cancer (Review)
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International Journal of Oncology.2017; 50(2): 345. CrossRef
MicroRNA-134 modulates glioma cell U251 proliferation and invasion by targeting KRAS and suppressing the ERK pathway
Yuguang Zhao, Dong Pang, Cui Wang, Shijiang Zhong, Shuang Wang
Tumor Biology.2016; 37(8): 11485. CrossRef
MicroRNA profiling of cisplatin-resistant oral squamous cell carcinoma cell lines enriched with cancer-stem-cell-like and epithelial-mesenchymal transition-type features
Ruma Dey Ghosh, Sangeeta Ghuwalewala, Pijush Das, Sapan Mandloi, Sk Kayum Alam, Jayanta Chakraborty, Sajal Sarkar, Saikat Chakrabarti, Chinmoy Kumar Panda, Susanta Roychoudhury
Scientific Reports.2016;[Epub] CrossRef
Cell‐free microRNAs in blood and other body fluids, as cancer biomarkers
Blanca Ortiz‐Quintero
Cell Proliferation.2016; 49(3): 281. CrossRef
Clinical relevance of circulating cell-free microRNAs in ovarian cancer
Koji Nakamura, Kenjiro Sawada, Akihiko Yoshimura, Yasuto Kinose, Erika Nakatsuka, Tadashi Kimura
Molecular Cancer.2016;[Epub] CrossRef
MicroRNA-432 functions as a tumor suppressor gene through targeting E2F3 and AXL in lung adenocarcinoma
Ling Chen, Guangming Kong, Chuantao Zhang, Hongyan Dong, Cuicui Yang, Guanhua Song, Chengye Guo, Lin Wang, Hongsheng Yu
Oncotarget.2016; 7(15): 20041. CrossRef
Hepatocellular carcinoma and microRNA: New perspectives on therapeutics and diagnostics
Ningning Yang, Nsikak R. Ekanem, Clement A. Sakyi, Sidhartha D. Ray
Advanced Drug Delivery Reviews.2015; 81: 62. CrossRef
Reduced expression of microRNA-134 correlates with malignancy and poor prognosis in human glioma
Jianfeng Zhong, Bing Li
Journal of Clinical Neuroscience.2015; 22(3): 583. CrossRef
2015 update on the diagnosis and management of neoplastic pericardial disease
Chiara Lestuzzi, Massimiliano Berretta, Witold Tomkowski
Expert Review of Cardiovascular Therapy.2015; 13(4): 377. CrossRef
Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer
Grit Rehbein, Bernd Schmidt, Michael Fleischhacker
Clinica Chimica Acta.2015; 450: 78. CrossRef

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Intrapleural Chemotherapy with Cisplatin and Cytarabine in the Management of Malignant Pleural Effusion: Kee Won Kim, Suk Young Park, Myung Sook Kim, Seok Chan Kim, Eun Hee Lee, So Young Shin, Jong Ho Lee, Jong Bum Kweon, Kuhn Park; Cancer Res Treat. 2004;36(1):68-71. Published online February 29, 2004; DOI: https://doi.org/10.4143/crt.2004.36.1.68

Abstract PDF PubReader ePub

Purpose

The purpose of this study was to evaluate the efficacy of intrapleural chemotherapy (IPC) with cisplatin and cytarabine in the management of malignant pleural effusion (MPE) from non-small-cell lung cancer (NSCLC).

Materials and Methods

A prospective analysis was carried out on 40 patients with pathologically proven MPE from NSCLC who had received IPC. A single dose of cisplatin 100 mg/m² plus cytarabine 1200 mg/m² in 250 ml normal saline was instilled into the pleural space via a chest tube and drained 4 hours later. Patients were evaluated for toxicities and responses at 1, 2, & 3 weeks and then at monthly intervals if possible. Systemic chemotherapy was administered, if the patient agreed to receive it, after achieving complete control (CC) of MPE.

Results

The median duration of chest tube insertion for drainage was 7 (3~32) days. Among the assessable 37 patients, CC and partial control (PC) were 32 (86.5%) and 4 (10.8%) patients, respectively (overall response rate 97.3%). The median duration of response was 12 months (2~23) and there were only two relapses of IPC after achieving CC. Among the 35 patients who were assessable until they died, 28 patients (80.0%) maintained CC until the last follow-up. There was only one toxic death and the toxicities of IPC, versus the results obtained, were deemed acceptable.

Conclusion

The procedures were tolerable to the patients and chemotherapy-induced complications were at an acceptable level. The outcome of this trial indicates that IPC has a superior and long lasting treatment response in the management of patients with MPE from NSCLC.

Citations

Citations to this article as recorded by

Efficacy of hyperthermic intrathoracic chemotherapy for initially diagnosed lung cancer with symptomatic malignant pleural effusion
Zihui Li, Jie Deng, Fei Yan, Li Liu, Yanling Ma, Jianhai Sun
Scientific Reports.2023;[Epub] CrossRef
Survival Benefits for Pulmonary Adenocarcinoma With Malignant Pleural Effusion After Thoracoscopic Surgical Treatment: A Real-World Study
Xin Li, Mingbiao Li, Jinshuang Lv, Jinghao Liu, Ming Dong, Chunqiu Xia, Honglin Zhao, Song Xu, Sen Wei, Zuoqing Song, Gang Chen, Hongyu Liu, Jun Chen
Frontiers in Oncology.2022;[Epub] CrossRef
Modified indwelling pleural catheter versus silver nitrate pleurodesis for the management of malignant pleural effusion
Mohammed F. Abdelghany, Khaled Essmat, Atef Farouk El-Karn, Sahar Farghly Youssif
The Egyptian Journal of Chest Diseases and Tuberculosis.2022; 71(2): 248. CrossRef
Chinese herbal injections versus intrapleural cisplatin for lung cancer patients with malignant pleural effusion: A Bayesian network meta-analysis of randomized controlled trials
Yi-Fang Xu, Yun-Ru Chen, Fan-Long Bu, Yu-Bei Huang, Yu-Xin Sun, Cheng-Yin Li, Jodi Sellick, Jian-Ping Liu, Dan-Mei Qin, Zhao-Lan Liu
Frontiers in Oncology.2022;[Epub] CrossRef
Making cold malignant pleural effusions hot: driving novel immunotherapies
Pranav Murthy, Chigozirim N. Ekeke, Kira L. Russell, Samuel C. Butler, Yue Wang, James D. Luketich, Adam C. Soloff, Rajeev Dhupar, Michael T. Lotze
OncoImmunology.2019; 8(4): e1554969. CrossRef
Efficacy and safety of intrapleural cisplatin versus silver nitrate in treatment of malignant pleural effusion
Mohammad K. El Badrawy, Raed El-Metwally Ali, Asem A. Hewidy, Mohamed A. El-Layeh, Fatma M. F. Akl, Abdelhadi Shebl
Egyptian Journal of Bronchology.2018; 12(1): 98. CrossRef
Biodegradable drug-eluting pellets provide steady and sustainable cisplatin release in the intrapleural cavity: In vivo and in vitro studies
Yin-Kai Chao, Yu-Wen Wen, Kuo-Sheng Liu, Yi-Chuan Wang, Chih-Wei Wang, Shih-Jung Liu
International Journal of Pharmaceutics.2015; 484(1-2): 38. CrossRef

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Cytogenetic Study in Suspicious Cases of Malignant Pleural Effusion: Seung Bum Han, Dae Kwang Kim; Cancer Res Treat. 2002;34(3):234-238. Published online June 30, 2002; DOI: https://doi.org/10.4143/crt.2002.34.3.234

Abstract PDF

PURPOSE
This study was performed to detect malignant cells in suspicious cases of malignant pleural effusion by cytogenetic analysis. MATERIALS AND METGODS: Eleven cases with pleural effusion were included in this study. Cells in pleural effusion were treated by direct, or short term, culture to prepare chromosomes. To analyze chromosomes, the G-banding method was used.
RESULTS
Chromosome preparations succeeded in 10 cases. 5 cases had normal karyotypes, but in 2 of these cases malignant cells were detected on cytological examination. The other 5 cases had abnormal chromosomes, but on cytological examination showed normal cell appearances.
CONCLUSION
Cytogenetic analysis of pleural effusions is not used routinely, but is more sensitive than the cytological examination of malignant pleural effusions. So, chromosome analysis is a good diagnostic tool, when chromosomal abnormalities are detected in an effusion. If a combination of cytology and cytogenetic study are used, the chance of detecting malignant cells in pleural effusion will be higher, and then more invasive diagnostic procedures, such as thoracoscopy or thoracotomy, could be avoided.

Citations

Citations to this article as recorded by

Sensitivity and complications of thoracentesis and thoracoscopy: a meta-analysis
Gabriela Martinez-Zayas, Sofia Molina, David E. Ost
European Respiratory Review.2022; 31(166): 220053. CrossRef

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1 Crossref

Localized Fibrous Tumor of the Pleura with a Very Prominent Small Cell Component - A case report -: Gil Hyun Kang, Jong Ok Kim, Bym Kyung Kim, Dae Young Kang, Kun Young Kwon; J Korean Cancer Assoc. 1995;27(6):1083-1089.

Abstract PDF: Localized fibrous tumors are rare neoplasms that most often involve the pleura The histogenesis is still controversial: specialized submesothelial or fibroblastic origin. The incidence has not been clearly linked to asbetos exposure. We experienced a case of solitary fibrous tumor of the pleura with a very prominent small cell component in a 68-year-old male. The mass was found in the right chest incidentally on routine chest X-ray. On chest CT, a well-circumscribed homogeneous low density mass was attached to the right parietal pleura and measured 10 x 9 x 7 cm in dimension. On operation, the mass was attached to the upper parietal pleura and focally adhered to the right upper lobe. Grossly, the mass was ovoid, firm, and encapsulated by a thin translucent glistening membrane, and measured 13,5x 9x 7cm in dimension. The cut surface was mostly grayish-yellow, firm, and focally variegated with hemorrhage, necrosis, cystic change, and myxoid area. Microscopically, irregular aggregates of small round cells predominated and were admixed with cellular spindle cell areas, dense collagenous areas, and angiofibroma-like areas. It also showed focal necrosis and mitosis of 1/10HPF. On immunohistochemical staining, vimentin was positive, but cytokeratin, EMA, LCA, chromogranin, and NSE were negative. Electron microscopy showed fusiform to round tumor cells with scanty cytoplasm in close apposition to focal collagen. The nuclei were euchromatic or heterochromatic. The cytoplasm contained a small amount of organelles. Focal primitive intercellular junctions were identified.

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Intrapleural Chemotherapy with Cisplatin and Cytarabine in the Manabement of Malignant Pleural Effusion: Suk Young Park, Baik Jong Seo; J Korean Cancer Assoc. 1996;28(4):698-705.

Abstract PDF: Malignant pleural effusion is a common snd signigicant problem in patients with advanced malignancies. The optimal means of control are not defined. Pleurodesis with tetracycline or other sclerosing agents has been the usual treatment for malignant pleural effusions. Intracavitary cisplatin-based chemotherapy has been recently reported to be successful in some clinical trials for the purpose of controlling malignant exudate and favored by some authors because it is thought to be cytotoxic rather than sclerosing, that is, the potential advantage of treating the underlying malignancy in addition to controlling the effusion. This study evaluated intrapleural cisplatin and cytarabine combination chemotherapy in 15 patients with malignant pleural effusions from lung and other cancers. A single dose of cisplatin plus cytarabine was instilled into pleural space via a chest tube and drained 4 hours later. Systemic chemotheapy and/or radiation therapy were given at least 3 weeks apart after intrapleural chemotherapy if decided to recieve them. The outcome of this trial indicated that the intrapleural chemotherapy had high and durable treatment response(87%; CR 13 cases(81%)) and the median duration of response was 29 weeks which seemed long enough to be little maiignant pieural effusion in 7 cases among 9 patients followed up till moribund state. The procedures were tolerable in all the cases and chemothrapy induced complications were not significant.

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Fibrinolytic Profiles in Malignant Pleural Effusion: Seung Ho Baik, Jin Woo Jeon, Jong Ho Won, Dae Sik Hong, Hee Sook Park; J Korean Cancer Assoc. 1996;28(4):705-710.

Abstract PDF: Plasminogen activators(PAs) and their inhibitors, plasminogen activator inhibitor-l(PAI-l) are known to play an important role in tumor invasion and metastasis. The differentiation of malignant pleural effusion(PE) from non-malignant pleural effusion is important for the management of a patient. Since malignant PE is a form of metastasis, and malignant cells are considered to be able to produce PAs or PAI-1, it seems reasonable to assume that PAa and PAI-1 in PE will be in somewhat different from that in non-malignant PE. We examined urokinase- type plasminogeo activator(uPA) and uPA receptor(uPAR) and tissue-type plasminogen activator(tPA), PAI-1 antigen using ELISA method and conventional laboratory tests and adenosine deaminase(ADA) in PEs. Levels of uPA, uPAR, tPA and PAI-1 were significantly increased in malignant and tuberculous PE than transudates(p<0.05) but not statistically significant between malignant and tuberculous PE. ADA in PE showed significantly increased in tuberculous PE than malignant PE and transudate(p<0.05). These data suggests that measurement of uPA, uPAR, tPA, PAI-1 antigen in addition to ADA could be helpful to differentiate malignant PE from tuberculous PE and transudate.

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