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HOME > J Korean Cancer Assoc > Volume 32(5); 2000 > Article
Original Article
The Effects of Interferon/Retinoic Acid on Cervical Cancer Cell Lines According to the Mutational Status of HPV-URR
Tae Yeon Kim, Chan Joo Kim, Eun Joo Kim, Tae Chul Park, Sung Eun Namkoong, Jae Gahb Park, Soo Jong Um, Jong Sup Park
Journal of the Korean Cancer Association 2000;32(5): 884-894.
1Department of Obstetrics & Gynecology, College of Medicine, The Catholic University of Korea, Seoul, Korea. jspark@cmc.cuk.ac.kr
2Department of Bioscience and Biotechnology, Sejong University, Seoul, Korea.
3Deptartment of Surgery, Seoul National University, Medical college, Seoul, Korea.
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PURPOSE
We investigated the effects of all-trans-retinoic acid (ATRA) and/or interferon-gamma (IFN-gamma) on the growth of various cervical cancer cell lines and HPV E6/E7 expression. The relationships between the functional activities of HPV-URR and the growth inhibition were identified.
MATERIALS AND METHODS
Four groups of cell lines were included; i) with integrated form of HPV-16 DNA (SNU-17, CaSki), ii) episomal form of HPV-16 (SNU-523), iii) integrated form of HPV-18 (SNU-1160, HeLa) and iv) episomal form of HPV-18 (SNU-1245). The promoter activity of HPV-URR was confirmed by transient transfection assay in C33A using the HPV-18 URR-CAT reporter plasmid.
RESULTS
Selective mutation was detected in TEF-1 (transcriptional enhancer factor) binding site in SNU-17, and the activity of URR in SNU-17 was higher than that of the prototype. The proliferation was more inhibited in SNU-17 by IFN-gamma (10 ng/ml) than in SNU-902, CaSki and HeLa. The increase of the HPV-URR activity might play a role in the inhibition of growth by interferon-g. The expression of HPV-16 E6/E7 were significantly decreased by ATRA or IFN-gamma.
CONCLUSION
Point mutation at TEF-1 binding site of SNU-17 was related with the increased transcriptional activity of URR. Mutation in the HPV-URR and alteration of HPV-URR activity in SNU-17 might be related with significant growth suppression by IFN-gamma.

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    The Effects of Interferon/Retinoic Acid on Cervical Cancer Cell Lines According to the Mutational Status of HPV-URR
    J Korean Cancer Assoc. 2000;32(5):884-894.
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