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Cancer Research and Treatment > Volume 33(5); 2001 > Article
Cancer Research and Treatment 2001;33(5): 385-391. doi: https://doi.org/10.4143/crt.2001.33.5.385
Effects of Polyamines on TNFalpha- or Tamoxifen-induced Apoptosis in Human Breast Cancer Cells
Ji Young Kim, Ki Young Kim, Kyeong Hee Lee, Ki Whan Hong, Byeong Gee Kim
1Division of Biological Sciences, College of NaturalSciences, Korea. bgkim@pnu.edu
2Department of Pharmacology, College of Medicine, PusanNational University, Busan, Korea.
  Published online: October 31, 2001.
To investigate the effects of polyamines on tumor necrosis factor alpha (TNFalpha)-or tamoxifen (TAM)-induced apoptosis in estrogen receptor (ER)-positive MCF- 7 and ER-negative MDA-MB-231 human breast cancer cells.
Cell viability was assessed by using MTT assay. Reactive oxygen species (ROS) generation was measured using 2', 7'-dichlorofluorescin diacetste (DCFDA) by fluorescence plate reader. DNA fragmentation was assessed by 1.5% agarose gel electrophoresis.
TNFalpah and TAM showed significant dose- and time- dependent inhibitory effects on the growth of MCF-7 human cells. However, the growth of MDA-MB-231 cells were not inhibited by TNFalpha or TAM treatment. The generation of ROS was increased in dose-and time-dependent manner by TNFalpha treatment in MCF-7 cells. Polyamines, especially spermine suppressed TNFalpha-induced ROS generation in MCF-7 cells. Antioxidant effects of polyamines were also demonstrated by DNA fragmentation, cell morphology as well as ROS generation assay. Polyamines also blocked TAM-induced cell death in MCF-7 cell. However, MDA-MB-231 cells showed resistance to the cytotoxic effects of TNFalpha or TAM.
These results suggest that polyamines may prevent TNFalpha or TAM-induced apoptosis in MCF-7 human breast cancer cells.
Key words: Polyamines;Apoptosis;TNFalpha;Tamoxifen
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