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Original Article
Gastrointestinal cancer
Clinical and Radiologic Predictors of Response to Atezolizumab-Bevacizumab in Advanced Hepatocellular Carcinoma
Se Jin Choi1orcid, Sung Won Chung2orcid, Jonggi Choi2, Kang Mo Kim2, Hyung-Don Kim3, Changhoon Yoo3, Baek-Yeol Ryoo3, Seung Soo Lee1, Won-Mook Choi2,orcid, Sang Hyun Choi1,orcid
Cancer Research and Treatment : Official Journal of Korean Cancer Association 2024;56(4):1219-1230.
DOI: https://doi.org/10.4143/crt.2024.283
Published online: May 7, 2024

1Department of Radiology and Research Institute of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

2Department of Gastroenterology, Liver Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

3Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

Correspondence: Sang Hyun Choi, Department of Radiology and Research Institute of Radiology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea
Tel: 82-2-3010-1797 Fax: 82-2-476-0090 E-mail: edwardchoi83@gmail.com
Co-correspondence: Won-Mook Choi, Department of Gastroenterology, Liver Center, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea
Tel: 82-2-3010-0048 Fax: 82-2-485-5782 E-mail: dr.choi85@gmail.com
*Se Jin Choi and Sung Won Chung contributed equally to this work.
• Received: March 9, 2024   • Accepted: May 4, 2024

Copyright © 2024 by the Korean Cancer Association

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Purpose
    This study aimed to identify clinical and radiologic characteristics that could predict response to atezolizumab-bevacizumab combination therapy in patients with advanced hepatocellular carcinoma (HCC).
  • Materials and Methods
    This single-center retrospective study included 108 advanced HCC patients with intrahepatic lesions who were treated with atezolizumab-bevacizumab. Two radiologists independently analyzed imaging characteristics of the index tumor on pretreatment computed tomography. Predictive factors associated with progressive disease (PD) at the best response based on Response Evaluation Criteria in Solid Tumors, ver. 1.1 were evaluated using logistic regression analysis. Progression-free survival (PFS) was estimated by the Kaplan-Meier method and compared with the log-rank test.
  • Results
    Of 108 patients with a median PFS of 15 weeks, 40 (37.0%) had PD during treatment. Factors associated with PD included the presence of extrahepatic metastases (adjusted odds ratio [aOR], 4.13; 95% confidence interval [CI], 1.19 to 14.35; p=0.03), the infiltrative appearance of the tumor (aOR, 3.07; 95% CI, 1.05 to 8.93; p=0.04), and the absence of arterial-phase hyperenhancement (APHE) (aOR, 6.34; 95% CI, 2.18 to 18.47; p < 0.001). Patients with two or more of these factors had a PD of 66.7% and a median PFS of 8 weeks, indicating a significantly worse outcome compared to the patients with one or no of these factors.
  • Conclusion
    In patients with advanced HCC treated with atezolizumab-bevacizumab treatment, the absence of APHE, infiltrative appearance of the intrahepatic tumor, and presence of extrahepatic metastases were associated with poor response and survival. Evaluation of early response may be necessary in patients with these factors.
Hepatocellular carcinoma (HCC) is among the most prevalent cancers worldwide and causes approximately 800,000 deaths annually, making it the third leading cause of cancer-related deaths [1]. Despite routine surveillance of populations at risk, many patients are still diagnosed with HCC at an advanced stage [2,3]. Numerous trials are being conducted on the treatment of advanced HCC due to its poor prognosis. The combination of the immune checkpoint inhibitor (ICI) atezolizumab and the vascular endothelial growth factor (VEGF) inhibitor bevacizumab has shown superior efficacy when compared with the tyrosine kinase inhibitor sorafenib [4], leading to the use of this combination as first-line therapy in patients with advanced HCC.
Trials in multiple malignancies have shown that only about 20%-40% of patients experience an objective tumor response to ICIs, with a smaller percentage achieving long-term remission [5-7]. Studies attempting to identify pretreatment predictors of response to ICIs have found that the level of expression of programmed death-ligand 1 (PD-L1) in tumor cells was associated with responses to treatment with ICIs, with high PD-L1 expression being associated with a favorable response to programmed death-1 (PD-1) or PD-L1 inhibitors [8]. Because the environment of HCC includes the interaction of hepatitis and/or cirrhosis with the host immune system, the HCC microenvironment is more complex than that of other solid tumors [9]. Indeed, it has been found that intrahepatic HCCs respond less favorably to ICIs than extrahepatic tumors [10], highlighting the importance of predicting the responses of intrahepatic HCCs to treatment with ICIs. Moreover, because HCC is primarily diagnosed based on imaging findings at high-risk setting (such as the presence of the hepatitis B or C virus or cirrhosis) [2,3,11], biopsies are not routinely performed, and pathologic prognostic factors such as PD-L1 expression may not be available in most HCC patients. Rather, because several imaging features of HCCs have been shown to correlate with their pathological characteristics, the potential role of radiologic findings as surrogate markers of pathologic data is currently under investigation [12].
Identifying pretreatment predictors based on clinical and radiologic findings of response to atezolizumab-bevacizumab could be keys to optimizing systemic therapy of HCC. The present study therefore sought to identify clinical and radiologic characteristics that could predict response to atezolizumab-bevacizumab combination therapy in patients with advanced HCC.
1. Study population
This retrospective study included patients who received atezolizumab-bevacizumab as first-line systemic treatment at a single tertiary referral hospital between July 1, 2016, and December 31, 2021. Patients previously enrolled in phase I trials or those who received atezolizumab-bevacizumab after the drugs were approved for treating unresectable HCC were evaluated for study eligibility. Patients were included according to the following criteria: (1) age ≥ 18 years; (2) HCC diagnosed by radiographic characteristics on computed tomography (CT) and/or magnetic resonance imaging (MRI) or histological findings, in accordance with the HCC guidelines of the American Association for the Study of Liver Diseases (AASLD) [3]; (3) advanced and unresectable HCC; (4) atezolizumab-bevacizumab treatment as first-line systemic treatment; and (5) available pretreatment multiphase liver CT within 1 month before atezolizumab-bevacizumab treatment. Patients were excluded if they (1) had been treated with either atezolizumab or bevacizumab alone; (2) had poor performance status, defined as an Eastern Cooperative Oncology Group (ECOG) performance status > 2; (3) had no evidence of HCC in the liver; or (4) had intrahepatic but nonmeasurable HCC (Fig. 1). Atezolizumab (fixed dose of 1,200 mg) plus bevacizumab (15 mg/kg) were administered intravenously every 3 weeks [4] until disease progression, severe adverse events, or death. According to patient tolerance, dosage delays were granted. Clinical information, including demographic characteristics, laboratory results, imaging characteristics, and clinical outcomes, was obtained from patients’ electronic medical records.
2. Image acquisition
All patients underwent pretreatment multiphase liver CT (Siemens Healthineers, Erlangen, Germany) within 1 month before atezolizumab-bevacizumab treatment. All imaging examinations met the technical acquisition standards of the Liver Imaging Reporting and Data System (LI-RADS) [13]. CT examinations were performed on 64 channel multidetector CT scanners. After unenhanced images were acquired, iodinated contrast medium (Ultravist 370, Bayer Schering Pharma, Berlin, Germany) was injected intravenously using a power injector at a rate of 3 mL/sec, followed by the acquisition of late-arterial phase (determined using a bolus triggering method), portal venous phase (70-90 seconds), and delayed phase (about 180 seconds) images. Details of the CT parameters are summarized in S1 Table.
3. Image analysis
All images were anonymized, randomized, and independently reviewed by two board-certified abdominal radiologists (with 8 and 4 years of experience in hepatic imaging, respectively). Because this study was mainly focused on the characteristics of index tumors on pretreatment CT, not on the diagnostic performance in lesion detection, the readers were informed that all patients had HCCs and were told the size and location of the index tumor to be analyzed. However, they were blinded to the clinical data, including imaging evaluations by other readers and treatment outcomes. The list of index tumors was prepared by an investigator who was not involved in the image analysis. When multiple tumors were present, the largest lesion was selected as the index tumor. Any discrepancies between the readers were settled by a consensus review with a third invited reader with 19 years of experience in hepatic imaging.
Imaging characteristics of the index tumor on pretreatment CT were evaluated, including tumor size, tumor location, the number of involved liver segments, tumor margins (smooth vs. nonsmooth), infiltrative appearance (yes vs. no), arterial-phase hyperenhancement (APHE; yes vs. no), rim enhancement (yes vs. no), washout appearance (yes vs. no), intratumoral necrosis (yes vs. no), peritumoral bile duct dilatation (yes vs. no), tumor-in-vein (yes vs. no), and multifocality (yes vs. no), as determined by LI-RADS lexicon [14]. Detailed definitions of the analyzed imaging characteristics are summarized in S2 Table.
4. Outcome assessment
Tumor response was assessed based on Response Evaluation Criteria in Solid Tumors, ver. 1.1 (RECIST 1.1). Responses were evaluated by serial multiphasic CT or MRI, performed after every two to three cycles of treatment (6-9 weeks), with additional evaluation as warranted. The primary outcome of interest was progressive disease (PD) at best response. The secondary outcome was progression-free survival (PFS).
5. Statistical analysis
Continuous variables were expressed as median and interquartile range or mean and standard deviation and were compared using unpaired two-tailed t-tests. Categorical variables were expressed as frequencies and percentages and were compared using Fisher’s exact test or the chi-square test. Inter-reader agreement on imaging characteristics was evaluated using intraclass correlation coefficients and kappa statistics.
To determine factors significantly predictive of PD, patients were classified into two groups (i.e., PD vs. non-PD), and univariable logistic regression analysis was performed using various clinical and imaging characteristics. Factors found to differ significantly on univariable analyses were included in a multivariable logistic regression analysis to determine factors independently predictive of PD after atezolizumab-bevacizumab treatment. Based on a multivariable logistic regression analysis, the risk for PD was presented graphically as a nomogram. The predictive accuracy of the nomogram was assessed using the concordance index (c-index), and a calibration plot was generated to compare the predicted and observed probability of PD by the nomogram. PFS was analyzed by the Kaplan-Meier method according to the presence or absence of a predictive factor and compared with the log-rank test. Hazard ratios (HRs) for survival outcomes were calculated using a Cox proportional hazards model. In addition, tumor response and PFS were compared according to the number of significant predictive factors (i.e., N=0, N=1, or N ≥ 2).
Statistical analyses were performed using SPSS ver. 21 (IBM Corp., Armonk, NY) or R statistics ver. 4.0.3 (R Foundation for Statistical Computing, Vienna, Austria), with p-values < 0.05 defined as statistically significant.
1. Patient characteristics
Of the 160 eligible patients, 52 were excluded, including six who received atezolizumab or bevacizumab monotherapy, 14 who were not followed up for assessment of treatment response, two with ECOG performance status > 2, 26 without no HCC in the liver, and four with nonmeasurable intrahepatic lesions. The study therefore included 108 patients, 95 men and 13 women, of mean age 58±12 years (Fig. 1). Their clinical characteristics are presented in Table 1. Of the 108 patients, 77 (71.3%) had hepatitis B, making it the most common risk factor for HCC; 55 (50.9%) had Child-Pugh class A disease; and 101 (93.5%) had advanced Barcelona Clinic Liver Cancer stage disease. Seventy-two patients (66.7%) received locoregional treatments, including ablation, radiation therapy, and transarterial chemoembolization, prior to treatment with atezolizumab-bevacizumab. Eighty patients (74.1%) had extrahepatic metastases, with the lungs being the most frequent sites of extrahepatic metastases.
During treatment, two patients (1.9%) achieved a complete response; 13 (12.0%) achieved a partial response; 53 (49.1%) had stable disease, making the disease control rate 63.0%; and 40 (37.0%) had PD at the best response. Over a median follow-up period of 8 months (maximum, 27 months), 87 patients (80.6%) died, and 88 (81.5%) experienced disease progression.
2. Pretreatment imaging characteristics
Pretreatment imaging characteristics are summarized in Table 2. Mean tumor sizes were 9.9±5.7 cm in the PD group and 8.3±5.4 cm in the non-PD group. The numbers of tumor-involved liver segments were similar (4.7±2.5 vs. 4.4±2.8, p=0.69), although infiltrative appearance was significantly more frequent in the PD than in the non-PD group (45.0% vs. 19.1%, p=0.005). In addition, absence of APHE (45.0% vs. 11.8%, p < 0.001) and presence of tumor-in-vein (70.0% vs. 38.2%, p=0.002) were significantly more frequent in the PD than in the non-PD group (Fig. 2).
The kappa value of inter-reader agreement for the infiltrative appearance, the absence of APHE, and tumor-in-vein was 0.60, 0.72, and 0.83, respectively (S3 Table).
3. Determination of factors predictive of progressive disease
Univariable analysis identified five clinical and imaging characteristics associated with PD: the presence of extrahepatic metastases, high α-fetoprotein (AFP; ≥ 400 ng/mL), the absence of APHE in the tumor, the infiltrative appearance of the tumor, and tumor-in-vein (Table 3). Multivariable analysis showed that the presence of extrahepatic metastases (adjusted odds ratio [aOR], 4.13; 95% confidence interval [CI], 1.19 to 14.35; p=0.03), the absence of APHE in the tumor (aOR, 6.34; 95% CI, 2.18 to 18.47; p < 0.001), and the infiltrative appearance of the tumor (aOR, 3.07; 95% CI, 1.05 to 8.93; p=0.04) were independent predictive factors of PD. Based on the results of this model, a nomogram was displayed showing the probability of PD (Fig. 3A). The model had a c-index of 0.78 (95% CI, 0.68 to 0.87), and the calibration plot showed a good calibration (Fig. 3B).
When comparing tumor response according to the number of significant predictive factors, the disease control rate was significantly lower in the group with two or more predictive factors compared to the group without predictive factors (33.3% vs. 83.3%, p < 0.001) or compared to the group with one predictive factor (33.3% vs. 78.4%, p < 0.001) (Table 4).
4. PFS stratified by significant predictive factors
Accordingly, median PFS was significantly shorter in patients with than without extrahepatic metastases (11.3 vs. 30.0 weeks; HR, 2.10; 95% CI, 1.29 to 3.42; p=0.03) (S4 Fig.), in patients without than with APHE (8.9 vs. 17.6 weeks; HR, 1.76; 95% CI, 1.11 to 2.79; p=0.02), and in patients with than without infiltrative appearance (8.3 vs. 17.1 weeks; HR, 1.65; 95% CI, 1.07 to 2.56; p=0.02).
Furthermore, when the patients were stratified by the number of significant predictive factors, the median PFS in the group with two or more predictive factors was significantly shorter than that both the group with one predictive factor (8.1 vs. 18.4 weeks; HR, 1.99; 95% CI, 1.03 to 3.86; p=0.04) and the group without predictive factors (8.1 vs. 37.9 weeks; HR, 3.80; 95% CI, 1.93 to 7.49; p < 0.001) (Fig. 4).
The present study analyzed baseline predictors of response to the combination of atezolizumab and bevacizumab in patients with advanced HCC. Although tumor markers did not differ significantly between the PD and non-PD groups, clinical and imaging characteristics, such as APHE in the tumor, the infiltrative appearance of the tumor, and the presence of extrahepatic metastases, were predictive of treatment response. In particular, when patients were stratified based on the number of predictive factors, those with two or more predictive factors had a disease control rate of only 33.3% and a median PFS of only 8 weeks, indicating a dismal outcome compared to those with one or no predictive factors.
Multitargeted tyrosine kinase inhibitors, including sorafenib, lenvatinib, regorafenib, and cabozantinib, often result in disease control with a modest response and have become the backbone of systemic therapy for HCC [15-18]. Most patients, however, develop resistance to these tyrosine kinase inhibitors after a certain period of time. The introduction of ICIs markedly altered the treatment landscape of HCC with a radiological response rate of 10%-20% with a durable response by anti–PD-1 monotherapy in patients with HCC [19,20]. The approval of the combination of atezolizumab-bevacizumab as first-line treatment for HCC was a significant milestone, leading to an increased response rate of up to 30% [4]. Although it remains unclear whether the combination of atezolizumab-bevacizumab elicits various organ-specific immune responses, tumor responses to ICIs have been reported to depend on the specific organ [21]. For example, intrahepatic HCCs were found to be less responsive to ICIs than extrahepatic metastases [10]. The progression of intrahepatic HCC is often accompanied by increased hepatic dysfunction, thus precluding the use of subsequent therapy and worsening prognosis. Therefore, predicting the response of intrahepatic HCC is crucial in managing the advanced HCC patients with intrahepatic lesions. The significance of this study lies in distinguishing between advanced HCC patients with a poor response and those with a favorable response based on pretreatment clinical and radiological characteristics, such as the absence of APHE, the infiltrative appearance of the tumor, and the presence of extrahepatic metastases.
Evaluating the presence of APHE in tumors is crucial for patients undergoing atezolizumab-bevacizumab treatment. In hepatocarcinogenesis, APHE in HCC can vary depending on the hepatic arterial blood supply. For instance, the absence of APHE (hypovascular HCC) may be observed in both poorly differentiated HCC and early HCC [22]. The VEGF inhibitor bevacizumab can block angiogenesis and cut off blood supply to the tumor, leading to severe hypoxia, deprivation of critical nutrients, and eventually necrosis [23]. Moreover, anti-VEGF therapy can enhance the efficacy of anti–PD-1 and anti–PD-L1 agents by reversing VEGF-mediated immunosuppression and promoting T-cell infiltration in tumors [24]. Considering that patients with more angiogenic tumors generally respond better to angiogenesis inhibitors [25,26], the absence of APHE in tumors could be an important and specific imaging feature for predicting PD after atezolizumab-bevacizumab treatment. A recent study indicated that patients with rim APHE demonstrated better response rates and PFS compared to those without rim APHE, with a higher proportion of CD8+ tumor-infiltrating lymphocytes [27]. However, due to the small number of patients with rim APHE in our study (8/108, 7.4%), our study had a limitation in evaluating whether rim APHE could be a significant predictor of better prognosis.
Furthermore, the presence of extrahepatic metastases indicates tumor spread and a high tumor burden. Generally, the likelihood of extrahepatic metastatic lesions increases with more advanced intrahepatic tumor stages. For example, one study found that 87% of patients with extrahepatic HCC had intrahepatic stages III (10%) and IVA (76%) tumors [28]. Moreover, characteristics associated with higher tumor burden, including the presence of extrahepatic metastases, were significant negative predictors of disease control in patients receiving nivolumab for advanced HCC [29].
Infiltrative HCC is very well known to have a poorer prognosis than nodular HCC as the former is characterized by a high tumor burden, the presence of vascular invasion and extrahepatic metastases, a high blood AFP level, and symptoms at presentation [30]. The growth rate of infiltrative HCC is generally very rapid, such that angiogenesis cannot catch up with tumor growth, reducing immune cell recruitment within the tumor and preventing a sufficient response to atezolizumab-bevacizumab treatment. While infiltrative appearance is well-known as an imaging characteristic indicative of a rapid growth pattern [12], the pathogenesis explaining its specific association with a poor response to atezolizumab-bevacizumab treatment is still lacking.
A previous study identified that hepatic vein thrombi and pleural effusion as factors independent prognostic of poor PFS [31], but these factors were not statistically significant in the present study. This discrepancy may be due to infiltrative HCC frequently involving tumor-in-vein, with the previous study not analyzing the presence of infiltrative appearance alone and patients with infiltrative tumors or irregular tumor margins grouped into a single category [31]. In the present study, however, 25 (80.6%) of 31 infiltrative HCCs had tumor-in-vein, with multivariable analysis finding that infiltrative appearance rather than tumor-in-vein was independently predictive of outcome. This previous study reported ascites, pleural effusion, and low density on CT as factors independent prognostic of poor overall survival [31]. Ascites and pleural effusion may be predictor of worse long-term survival in cirrhotic patients, suggesting decompensated liver disease or the presence of coexisting cardiac disease [32,33]. Also, the low density of HCC may be a predictor of poor overall survival, as it has been associated with poorly differentiated HCC [34]. However, given the conflicting results for PFS and the lack of evaluation of overall survival in our study, further research is necessary to confirm these findings.
This study had several limitations. First, the retrospective design of this study and its inclusion of patients at a single center indicated that selection bias could not be ruled out completely. Most patients were registered before atezolizumab-bevacizumab combination therapy was approved for national insurance coverage; thus, some patients may not have been treated with these agents for financial reasons, introducing a selection bias. Second, this study included patients who received atezolizumab-bevacizumab between January 2016 and December 2021. Because the purpose of this study was to analyze the response after atezolizumab-bevacizumab treatment, it was necessary to include eligible patients who have a sufficient follow-up period. Third, due to the small number of patients and the analysis limited to CT (i.e., not included MRI), it may limit the general applicability of these study results. Particularly, this study was able to identify predictors of poor response to treatment but could not identify the clinical and imaging characteristics associated with favorable responses in patients with long-lasting CR and PR. Additional studies with larger populations are required to determine the clinical and imaging characteristics of patients with good responses to atezolizumab-bevacizumab treatment. Fourth, the lack of histopathological correlations can hinder a comprehensive understanding of the significant imaging features observed. Last, the results of the present study cannot be extrapolated to estimate whether patients who are predicted to have a poor response to atezolizumab-bevacizumab combination therapy will benefit from alternative treatment with multi-tyrosine kinase inhibitors such as sorafenib or lenvatinib, and further research is needed to address this issue.
In conclusion, the present study identified several pretreatment clinical and imaging characteristics that were associated with poor response in advanced HCC patients with intrahepatic lesions who were treated with a combination of atezolizumab and bevacizumab. These factors included the absence of APHE, the infiltrative appearance of the tumor, and the presence of extrahepatic metastases. These pretreatment factors may guide the selection of patients who would benefit from atezolizumab-bevacizumab treatment. Further studies are warranted to validate the findings of the present study.
Supplementary materials are available at Cancer Research and Treatment website (https://www.e-crt.org).

Ethical Statement

The study protocol was in accordance with the guidelines of the Declaration of Helsinki and was approved by the Institutional Review Board of Asan Medical Center (approval no.: 2022-0101), which waived the requirement for informed patient consent due to the retrospective nature of this study.

Author Contributions

Conceived and designed the analysis: Choi SH, Choi WM.

Collected the data: Choi J, Kim KM, Kim HF, Yoo C, Ryoo BY.

Contributed data or analysis tools: Choi SJ, Chung SW.

Performed the analysis: Choi SJ, Chung SW.

Wrote the paper: Choi SJ, Chung SW, Choi J, Kim KM, Kim HD, Yoo C, Ryoo BY, Lee SS, Choi SH, Choi WM.

Visualization: Lee SS.

Conflict of Interest

Conflict of interest relevant to this article was not reported.

Fig. 1.
Flow diagram for patient enrollment. AASLD, American Association for the Study of Liver Diseases; CR, complete response; CT, computed tomography; ECOG, Eastern Cooperative Oncology Group; HCC, hepatocellular carcinoma; PD, progressive disease; PR, partial response; SD, stable disease.
crt-2024-283f1.jpg
Fig. 2.
A 57-year-old male with biopsy proven hepatocellular carcinoma in the left hepatic lobe. (A-C) Pretreatment dynamic contrast-enhanced computed tomography showing a 9.8 cm infiltrative mass (arrow) without arterial-phase hyperenhancement (A). Nonsmooth tumor margins (arrows) on portal venous (B) and delayed-phase (C) images with tumor-in-vein in the left portal vein (arrowheads). (D) Metastatic nodules in the left lower lobe (arrow). This patient experienced disease progression after 12.5 weeks.
crt-2024-283f2.jpg
Fig. 3.
Nomogram for predicting progressive disease. (A) The nomogram depicts the predicted probability of progressive disease on a scale of 0 to 280, derived from a multivariable logistic regression model. (B) Calibration plot for predicted probability vs. actual probability for progressive disease. The dotted line indicates predicted probability, while the black line represents the actual probability, and the gray zone indicates the 95% confidence intervals. APHE, arterial-phase hyperenhancement.
crt-2024-283f3.jpg
Fig. 4.
Kaplan-Meier analyses of progression-free survival outcomes according to the number of significant predictive factors (N=0, N=1, or N ≥ 2). Significant predictive factors include the absence of APHE, the infiltrative appearance of the tumor, and the presence of extrahepatic metastases.
crt-2024-283f4.jpg
Table 1.
Clinical characteristics of the 108 patients included in this study
Variable Value
Age (yr) 58±12
Sex
 Men 95 (88.0)
 Women 13 (12.0)
Etiology
 HBV 77 (71.3)
 HCV 6 (5.6)
 Others 25 (23.1)
Child-Pugh class
 A 55 (50.9)
 B 53 (49.1)
ECOG performance status
 0 11 (10.2)
 1 94 (87.0)
 2 3 (2.8)
Ascites 10 (9.3)
Pleural effusion 10 (9.3)
Prior treatment
 Surgery 28 (25.9)
 Ablation 13 (12.0)
 TACE 69 (63.9)
 RT 41 (38.0)
BCLC stage
 Intermediate 7 (6.5)
 Advanced 101 (93.5)
Extrahepatic metastasis 80 (74.1)
Involved organ
 Lung 54 (50.0)
 Bone 17 (15.7)
 Lymph node 26 (24.1)
Multiorgan metastases 25 (23.2)
Laboratory data
 Albumin (g/dL) 3.5 (3.2, 3.9)
 Bilirubin (g/dL) 0.8 (0.5, 1.1)
 INR 1.1 (1.0, 1.2)
ALBI grade
 Grade 1 21 (19.4)
 Grade 2 83 (76.9)
 Grade 3 4 (3.7)
Tumor markers
 AFP (ng/mL) 359 (15-4,463)
 PIVKA-II (mAU/mL) 784 (121-7,587)

Values are resented as mean±SD, median (IQR), or number (%). AFP, α-fetoprotein; ALBI, albumin-bilirubin; BCLC, Barcelona Clinic Liver Cancer; ECOG, Eastern Cooperative Oncology Group; HBV, hepatitis B virus; HCV, hepatitis C virus; INR, international normalized ratio; IQR, interquartile range; PIVKAII, protein induced by vitamin K absence or antagonist-II; RT, radiation therapy; SD, standard deviation; TACE, transarterial chemoembolization.

Table 2.
Pretreatment imaging characteristics of the index tumor
Variable PD (n=40) Non-PD (n=68) p-value
Tumor size (cm) 9.9±5.7 8.3±5.4 0.13
No. of involved segments 4.7±2.5 4.4±2.8 0.69
Tumor margin
 Smooth 13 (32.5) 31 (45.6) 0.18
 Nonsmooth 27 (67.5) 37 (54.4)
Infiltrative appearance
 Present 18 (45.0) 13 (19.1) 0.005
 Absent 22 (55.0) 55 (80.9)
APHE
 Present 22 (55.0) 60 (88.2) < 0.001
 Absent 18 (45.0) 8 (11.8)
Rim enhancement
 Present 4 (10.0) 4 (5.9) 0.43
 Absent 36 (90.0) 64 (94.1)
Washout appearance
 Present 36 (90.0) 53 (77.9) 0.12
 Absent 4 (10.0) 15 (22.1)
Intratumoral necrosis
 Present 17 (42.5) 28 (41.2) 0.89
 Absent 23 (57.5) 40 (58.8)
Peritumoral bile duct dilatation
 Present 3 (7.5) 7 (10.3) 0.63
 Absent 37 (92.5) 61 (89.7)
Tumor-in-vein
 Present 28 (70.0) 26 (38.2) 0.002
 Absent 12 (30.0) 42 (61.7)
Multifocality in liver
 Present 29 (72.5) 46 (67.6) 0.60
 Absent 11 (27.5) 22 (32.4)

Values are resented as mean±SD or number (%). APHE, arterial-phase hyperenhancement; PD, progressive disease; SD, standard deviation.

Table 3.
Univariable and multivariable analyses of factors predictive of progressive disease
Variable Univariable analysis
Multivariable analysis
OR (95% CI) p-value OR (95% CI) p-value
Age, per 1-year increase 0.99 (0.96-1.03) 0.72
Sex
 Women 1 (reference) 0.28
 Men 2.13 (0.55-8.24)
Etiology
 Non-HBV 1 (reference) 0.83
 HBV 1.10 (0.46-2.62)
Child-Pugh class
 A 1 (reference) 0.52
 B 0.77 (0.35-1.69)
ECOG performance
 0 or 1 1 (reference) 0.19
 2 2.87 (0.59-14.03)
Ascites
 Present 1.15 (0.30-4.34) 0.84
Pleural effusion
 Present 1.80 (0.49-6.65) 0.38
Prior treatment
 Surgery 1.39 (0.58-3.35) 0.46
 Ablation 1.54 (0.48-4.95) 0.47
 TACE 1.08 (0.48-2.44) 0.85
 RT 1.60 (0.72-3.56) 0.25
BCLC stage
 B 1 (reference) 0.23
 C 3.77 (0.44-32.55)
Extrahepatic metastasis
 Present 3.58 (1.24-10.36) 0.02 4.13 (1.19-14.35) 0.03
Involved organ
 Lung 2.65 (1.18-5.96) 0.02
 Bone 2.18 (0.77-6.20) 0.15
 Lymph node 1.08 (0.44-2.69) 0.86
Multiorgan metastases
 Present 1.65 (0.68-4.05) 0.27
ALBI grade
 Grade 1 1 (reference) 0.54
 Grade 2 or 3 0.74 (0.28-1.95)
AFP (ng/mL)
 < 400 1 (reference) 0.03
 ≥ 400 2.38 (1.07-5.31) 1.55 (0.59-4.07) 0.38
PIVKA-II (mAU/mL)
 < 2,000 1 (reference) 0.10
 ≥ 2,000 1.93 (0.88-4.26)
Tumor size, per 1 cm increase 1.06 (0.98-1.13) 0.13
No. of involved segments 1.03 (0.89-1.20) 0.69
Tumor margin
 Smooth 1 (reference) 0.18
 Nonsmooth 1.74 (0.77-3.93)
Infiltrative appearance
 Present 3.46 (1.45-8.25) 0.005 3.07 (1.05-8.93) 0.04
APHE
 Absent 6.14 (2.34-16.12) < 0.001 6.34 (2.18-18.47) < 0.001
Rim enhancement
 Present 1.88 (0.44-8.00) 0.43
Washout appearance
 Present 2.55 (0.78-8.30) 0.12
Intratumoral necrosis
 Present 1.06 (0.48-2.33) 0.89
Peritumoral bile duct dilatation
 Present 0.71 (0.17-2.90) 0.63
Tumor-in-vein
 Present 3.77 (1.64-8.68) 0.002 2.07 (0.74-5.77) 0.16
Multifocality in liver
 Present 1.26 (0.53-2.98) 0.60

AFP, α-fetoprotein; ALBI, albumin-bilirubin; APHE, arterial-phase hyperenhancement; BCLC, Barcelona Clinic Liver Cancer; CI, confidence interval; ECOG, Eastern Cooperative Oncology Group; HBV, hepatitis B virus; OR, odds ratio; PIVKA-II, protein induced by vitamin K absence or antagonist-II; RT, radiation therapy; TACE, transarterial chemoembolization.

Table 4.
Treatment response and disease control rate according to the number of significant predictive factors
Treatment response No. of significant predictive factors
p-value
N=0 (n=18) N=1 (n=51) N ≥ 2 (n=39)
Complete response 1 0 1
Partial response 5 6 2
Stable disease 9 34 10
Progressive disease 3 11 26
Disease control rate (%) 83.3 78.4 33.3 < 0.001
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      Cancer Res Treat. 2024;56(4):1219-1230.   Published online May 7, 2024
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    Clinical and Radiologic Predictors of Response to Atezolizumab-Bevacizumab in Advanced Hepatocellular Carcinoma
    Image Image Image Image
    Fig. 1. Flow diagram for patient enrollment. AASLD, American Association for the Study of Liver Diseases; CR, complete response; CT, computed tomography; ECOG, Eastern Cooperative Oncology Group; HCC, hepatocellular carcinoma; PD, progressive disease; PR, partial response; SD, stable disease.
    Fig. 2. A 57-year-old male with biopsy proven hepatocellular carcinoma in the left hepatic lobe. (A-C) Pretreatment dynamic contrast-enhanced computed tomography showing a 9.8 cm infiltrative mass (arrow) without arterial-phase hyperenhancement (A). Nonsmooth tumor margins (arrows) on portal venous (B) and delayed-phase (C) images with tumor-in-vein in the left portal vein (arrowheads). (D) Metastatic nodules in the left lower lobe (arrow). This patient experienced disease progression after 12.5 weeks.
    Fig. 3. Nomogram for predicting progressive disease. (A) The nomogram depicts the predicted probability of progressive disease on a scale of 0 to 280, derived from a multivariable logistic regression model. (B) Calibration plot for predicted probability vs. actual probability for progressive disease. The dotted line indicates predicted probability, while the black line represents the actual probability, and the gray zone indicates the 95% confidence intervals. APHE, arterial-phase hyperenhancement.
    Fig. 4. Kaplan-Meier analyses of progression-free survival outcomes according to the number of significant predictive factors (N=0, N=1, or N ≥ 2). Significant predictive factors include the absence of APHE, the infiltrative appearance of the tumor, and the presence of extrahepatic metastases.
    Clinical and Radiologic Predictors of Response to Atezolizumab-Bevacizumab in Advanced Hepatocellular Carcinoma
    Variable Value
    Age (yr) 58±12
    Sex
     Men 95 (88.0)
     Women 13 (12.0)
    Etiology
     HBV 77 (71.3)
     HCV 6 (5.6)
     Others 25 (23.1)
    Child-Pugh class
     A 55 (50.9)
     B 53 (49.1)
    ECOG performance status
     0 11 (10.2)
     1 94 (87.0)
     2 3 (2.8)
    Ascites 10 (9.3)
    Pleural effusion 10 (9.3)
    Prior treatment
     Surgery 28 (25.9)
     Ablation 13 (12.0)
     TACE 69 (63.9)
     RT 41 (38.0)
    BCLC stage
     Intermediate 7 (6.5)
     Advanced 101 (93.5)
    Extrahepatic metastasis 80 (74.1)
    Involved organ
     Lung 54 (50.0)
     Bone 17 (15.7)
     Lymph node 26 (24.1)
    Multiorgan metastases 25 (23.2)
    Laboratory data
     Albumin (g/dL) 3.5 (3.2, 3.9)
     Bilirubin (g/dL) 0.8 (0.5, 1.1)
     INR 1.1 (1.0, 1.2)
    ALBI grade
     Grade 1 21 (19.4)
     Grade 2 83 (76.9)
     Grade 3 4 (3.7)
    Tumor markers
     AFP (ng/mL) 359 (15-4,463)
     PIVKA-II (mAU/mL) 784 (121-7,587)
    Variable PD (n=40) Non-PD (n=68) p-value
    Tumor size (cm) 9.9±5.7 8.3±5.4 0.13
    No. of involved segments 4.7±2.5 4.4±2.8 0.69
    Tumor margin
     Smooth 13 (32.5) 31 (45.6) 0.18
     Nonsmooth 27 (67.5) 37 (54.4)
    Infiltrative appearance
     Present 18 (45.0) 13 (19.1) 0.005
     Absent 22 (55.0) 55 (80.9)
    APHE
     Present 22 (55.0) 60 (88.2) < 0.001
     Absent 18 (45.0) 8 (11.8)
    Rim enhancement
     Present 4 (10.0) 4 (5.9) 0.43
     Absent 36 (90.0) 64 (94.1)
    Washout appearance
     Present 36 (90.0) 53 (77.9) 0.12
     Absent 4 (10.0) 15 (22.1)
    Intratumoral necrosis
     Present 17 (42.5) 28 (41.2) 0.89
     Absent 23 (57.5) 40 (58.8)
    Peritumoral bile duct dilatation
     Present 3 (7.5) 7 (10.3) 0.63
     Absent 37 (92.5) 61 (89.7)
    Tumor-in-vein
     Present 28 (70.0) 26 (38.2) 0.002
     Absent 12 (30.0) 42 (61.7)
    Multifocality in liver
     Present 29 (72.5) 46 (67.6) 0.60
     Absent 11 (27.5) 22 (32.4)
    Variable Univariable analysis
    Multivariable analysis
    OR (95% CI) p-value OR (95% CI) p-value
    Age, per 1-year increase 0.99 (0.96-1.03) 0.72
    Sex
     Women 1 (reference) 0.28
     Men 2.13 (0.55-8.24)
    Etiology
     Non-HBV 1 (reference) 0.83
     HBV 1.10 (0.46-2.62)
    Child-Pugh class
     A 1 (reference) 0.52
     B 0.77 (0.35-1.69)
    ECOG performance
     0 or 1 1 (reference) 0.19
     2 2.87 (0.59-14.03)
    Ascites
     Present 1.15 (0.30-4.34) 0.84
    Pleural effusion
     Present 1.80 (0.49-6.65) 0.38
    Prior treatment
     Surgery 1.39 (0.58-3.35) 0.46
     Ablation 1.54 (0.48-4.95) 0.47
     TACE 1.08 (0.48-2.44) 0.85
     RT 1.60 (0.72-3.56) 0.25
    BCLC stage
     B 1 (reference) 0.23
     C 3.77 (0.44-32.55)
    Extrahepatic metastasis
     Present 3.58 (1.24-10.36) 0.02 4.13 (1.19-14.35) 0.03
    Involved organ
     Lung 2.65 (1.18-5.96) 0.02
     Bone 2.18 (0.77-6.20) 0.15
     Lymph node 1.08 (0.44-2.69) 0.86
    Multiorgan metastases
     Present 1.65 (0.68-4.05) 0.27
    ALBI grade
     Grade 1 1 (reference) 0.54
     Grade 2 or 3 0.74 (0.28-1.95)
    AFP (ng/mL)
     < 400 1 (reference) 0.03
     ≥ 400 2.38 (1.07-5.31) 1.55 (0.59-4.07) 0.38
    PIVKA-II (mAU/mL)
     < 2,000 1 (reference) 0.10
     ≥ 2,000 1.93 (0.88-4.26)
    Tumor size, per 1 cm increase 1.06 (0.98-1.13) 0.13
    No. of involved segments 1.03 (0.89-1.20) 0.69
    Tumor margin
     Smooth 1 (reference) 0.18
     Nonsmooth 1.74 (0.77-3.93)
    Infiltrative appearance
     Present 3.46 (1.45-8.25) 0.005 3.07 (1.05-8.93) 0.04
    APHE
     Absent 6.14 (2.34-16.12) < 0.001 6.34 (2.18-18.47) < 0.001
    Rim enhancement
     Present 1.88 (0.44-8.00) 0.43
    Washout appearance
     Present 2.55 (0.78-8.30) 0.12
    Intratumoral necrosis
     Present 1.06 (0.48-2.33) 0.89
    Peritumoral bile duct dilatation
     Present 0.71 (0.17-2.90) 0.63
    Tumor-in-vein
     Present 3.77 (1.64-8.68) 0.002 2.07 (0.74-5.77) 0.16
    Multifocality in liver
     Present 1.26 (0.53-2.98) 0.60
    Treatment response No. of significant predictive factors
    p-value
    N=0 (n=18) N=1 (n=51) N ≥ 2 (n=39)
    Complete response 1 0 1
    Partial response 5 6 2
    Stable disease 9 34 10
    Progressive disease 3 11 26
    Disease control rate (%) 83.3 78.4 33.3 < 0.001
    Table 1. Clinical characteristics of the 108 patients included in this study

    Values are resented as mean±SD, median (IQR), or number (%). AFP, α-fetoprotein; ALBI, albumin-bilirubin; BCLC, Barcelona Clinic Liver Cancer; ECOG, Eastern Cooperative Oncology Group; HBV, hepatitis B virus; HCV, hepatitis C virus; INR, international normalized ratio; IQR, interquartile range; PIVKAII, protein induced by vitamin K absence or antagonist-II; RT, radiation therapy; SD, standard deviation; TACE, transarterial chemoembolization.

    Table 2. Pretreatment imaging characteristics of the index tumor

    Values are resented as mean±SD or number (%). APHE, arterial-phase hyperenhancement; PD, progressive disease; SD, standard deviation.

    Table 3. Univariable and multivariable analyses of factors predictive of progressive disease

    AFP, α-fetoprotein; ALBI, albumin-bilirubin; APHE, arterial-phase hyperenhancement; BCLC, Barcelona Clinic Liver Cancer; CI, confidence interval; ECOG, Eastern Cooperative Oncology Group; HBV, hepatitis B virus; OR, odds ratio; PIVKA-II, protein induced by vitamin K absence or antagonist-II; RT, radiation therapy; TACE, transarterial chemoembolization.

    Table 4. Treatment response and disease control rate according to the number of significant predictive factors


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