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Cancer Research and Treatment > Accepted Articles
doi: https://doi.org/10.4143/crt.2022.1647    [Accepted]
Genomic and Transcriptomic Characterization Revealed the High Sensitivity of Targeted Therapy and Immunotherapy in a Subset of Endometrial Stromal Sarcoma
Nan Kang1 , Yinli Zhang1, Shichao Guo2, Ran Chen1, Fangzhou Kong1, Shuchun Wang3, Mingming Yuan3, Rongrong Chen3, Danhua Shen1, Jianliu Wang4
1Department of Pathology, Peking University People’s Hospital, Beijing, China
2State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
3Geneplus-Beijing, Beijing, China
4Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, China
Correspondence  Danhua Shen ,Email: shenpath59@163.com
Jianliu Wang ,Email: wangjianliupkuph@edu.cn
Received: December 23, 2022;  Accepted: February 1, 2023.  Published online: February 2, 2023.
The unique chromosomal rearrangements of endometrial stromal sarcoma (ESS) make it possible to distinguish high-grade ESS (HGESS) and low-grade ESS (LGESS) from the molecular perspective. Analysis of ESS at the genomic and transcriptomic levels can help us achieve accurate diagnosis of ESS and provide potential therapy options for ESS patients.
Materials and Methods
A total of 36 ESS patients who conducted DNA- and/or RNA-based next generation sequencing were retrospectively enrolled in this study. The molecular characteristics of ESS at genomic and transcriptomic levels, including mutational spectrum, fusion profiles, gene expression and pathway enrichment analysis and features about immune microenvironment were comprehensively explored.
TP53 and DNMT3A mutations were the most frequent mutations. The classical fusions frequently found in HGESS (ZC3H7B-BCOR and NUTM2B-YWHAE) and LGESS (JAZF1-SUZ12) were detected in our cohort. CCND1 was significantly up-regulated in HGESS, while the expression of GPER1 and PGR encoding ER and PR did not differ significantly between HGESS and LGESS. Actionable mutations enriched in homologous recombination repair, cell cycle and PI3K/AKT/mTOR pathways were detected in 60% of HGESS patients. Genes with up-regulated expression in HGESS were significantly enriched in five immune-related pathways. Most HGESS patients (85.7%) had positive predictors of immunotherapy efficacy. Moreover, immune microenvironment analysis showed that HGESS had relatively high immune infiltration. The degree of immune infiltration in HGESS patients with ZC3H7B-BCOR fusion was relatively higher than that those with NUTM2B-YWHAE fusion.
This study investigated the molecular characteristics of ESS patients at the genomic and transcriptomic levels and revealed the potentially high sensitivity of targeted therapy and immunotherapy in a subset of HGESS with specific molecular features, providing a basis for guiding decision-making of treatment and the design of future clinical trials on precision therapy.
Key words: DNA-based panel sequencing, High-grade endometrial stromal sarcoma, Immunotherapy, Targeted therapy, Whole Transcriptome Sequencing
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