Junghoon Shin; Young Hyeh Ko; Sung Yong Oh; Dok Hyun Yoon; Jeong-Ok Lee; Jin Seok Kim; Yong Park; Ho Jin Shin; Seok Jin Kim; Jong Ho Won; Sung-Soo Yoon; Won Seog Kim; Youngil Koh

doi:10.4143/crt.2018.555

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Original Article Body Cavity–Based Lymphoma in a Country with Low Human Immunodeficiency Virus Prevalence: A Series of 17 Cases from the Consortium for Improving Survival of Lymphoma: Junghoon Shin, MD¹, Young Hyeh Ko, MD, PhD², Sung Yong Oh, MD, PhD³, Dok Hyun Yoon, MD, PhD⁴, Jeong-Ok Lee, MD, PhD⁵, Jin Seok Kim, MD, PhD⁶, Yong Park, MD, PhD⁷, Ho Jin Shin, MD, PhD⁸, Seok Jin Kim, MD, PhD⁹, Jong Ho Won, MD, PhD¹⁰, Sung-Soo Yoon, MD, PhD¹, Won Seog Kim, MD, PhD^9,, Youngil Koh, MD, PhD^1,; Cancer Research and Treatment : Official Journal of Korean Cancer Association 2019;51(4):1302-1312.
DOI: https://doi.org/10.4143/crt.2018.555
Published online: February 14, 2019

¹Department of Internal Medicine, Seoul National University Hospital, Seoul, Korea

²Department of Pathology, Samsung Medical Center, Seoul, Korea

³Department of Internal Medicine, Dong-A University College of Medicine, Busan, Korea

⁴Department of Oncology, Asan Medical Center, Seoul, Korea

⁵Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea

⁶Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea

⁷Department of Internal Medicine, Korea University Anam Hospital, Seoul, Korea

⁸Department of Internal Medicine, Pusan National University Hospital, Busan, Korea

⁹Department of Medicine, Samsung Medical Center, Seoul, Korea

¹⁰Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, Korea

Correspondence: Won Seog Kim, MD, PhD Department of Medicine, Samsung Medical Center, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea
Tel: 82-2-3410-6548 Fax: 82-2-3410-1754 E-mail: wskimsmc@skku.edu

Co-correspondence: Youngil Koh, MD, PhD Department of Internal Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea
Tel: 82-2-2072-7217 Fax: 82-2-2072-7379 E-mail: go01@snu.ac.kr

• Received: October 8, 2018 • Accepted: January 28, 2019

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Abstract

Purpose
Primary effusion lymphoma (PEL) is a type of body cavity–based lymphoma (BCBL). Most patients with PEL are severely immunocompromised and seropositive for human immunodeficiency virus (HIV). We investigated the distinctive clinicopathologic characteristics of BCBL in a country with low HIV burden.
Materials and Methods
We retrospectively collected data on the clinicopathologic characteristics, treatments, and outcomes of 17 consecutive patients with BCBL at nine institutions in Korea.
Results
Latency-associated nuclear antigen 1 (LANA1) immunostaining indicated that six patients had PEL, six patients had human herpesvirus 8 (HHV8)-unrelated BCBL, and five patients had HHV8-unknown BCBL. The patients with PEL exhibited no evidence of immunodeficiency except for one who was HIV positive. One (20%) and four (80%) patients with PEL and six (100%) and zero (0%) patients with HHV8-unrelated BCBL were positive for CD20 and CD30 expression, respectively. The two patients with PEL (one HIV-positive and one HIV-negative patient) with the lowest proliferation activity as assessed by the Ki-67 labeling index survived for > 1 and > 4 years without chemotherapy, respectively, in contrast to the PEL cases in the literature, which mostly showed a high proliferation index and poor survival.
Conclusion
PEL mostly occurred in ostensibly immunocompetent individuals and had a favorable outcome in Korea. A watchful waiting approach may be applicable for managing HIV-seronegative patients with PEL with a low Ki-67 labeling index. A possible trend was detected among LANA1, CD20, and CD30 expression in BCBL.
Key words: Body cavity-based lymphoma, Primary effusion lymphoma, Human herpesvirus 8, Human immunodeficiency virus, Immunophenotype

Introduction

Primary effusion lymphoma (PEL) is a rare subtype of non-Hodgkin lymphoma (NHL) characterized by lymphomatous effusions in body cavities with or without extracavitary tumor formation [1]. Following the discovery of its universal association with human herpesvirus 8 (HHV8) infection [2], PEL was established as a distinct clinical entity and incorporated into the World Health Organization (WHO) classification system for lymphoid neoplasms [3,4]. PEL mostly occurs in patients with human immunodeficiency virus (HIV) infection [2,4]. In the four largest published series of PEL cases, each including 11-34 patients with classic (body cavity-based) PEL, more than 90% of the patients were young-to-middle-aged males who had HIV risk factors such as homosexuality or intravenous drug abuse [5-8]. Although non-HIV–associated PEL has occasionally been observed, the patients were either elderly (≥ 78 years of age) or recipients of an organ transplant, or the disease developed in an artificial cavity related to a breast implant capsule, suggesting that systemic or local immunocompromising conditions were involved in the lymphomagenesis [4,9-11]. On the basis of those observations, it is considered unusual for PEL to occur in immunocompetent individuals.

Since the establishment of PEL as a subcategory of NHL, there have been numerous reports of HHV8-unrelated body cavity-based lymphoma (BCBL) [12-18], a provisional entity that does not fulfill the diagnostic criteria of PEL because of the absence of HHV8 infection in neoplastic cells [3]. HHV8-unrelated BCBL has a remarkable clinicopathologic similarity to PEL in terms of its effusion-based growth and its large, pleomorphic, immunoblastic-to-anaplastic cytomorphology [17,18]; however, it differs from PEL in important aspects: (1) it commonly affects immunocompetent individuals and females; (2) the tumor cells often express pan-B-cell markers including CD19, CD20, and CD79a; and (3) the combination chemotherapy with or without rituximab shows promising efficacy, resulting in a better prognosis compared with that of PEL [16-18]. Therefore, it is important to differentiate between HHV8-unrelated BCBL and PEL to ensure proper management and prognostication of patients.

Because HIV/acquired immunodeficiency syndrome (AIDS) confers significant morbidity and mortality due to opportunistic infections and various malignancies, HIV-unrelated PEL may exhibit distinctive clinical features and have a different prognosis compared with typical HIV-related PEL. Most, if not all, patients with PEL have been described in North America and Europe [4-8], whereas around 60% of patients described with HHV8-unrelated BCBL are Japanese [16-18]. That remarkable imbalance in case distribution is in line with the low prevalence of HIV/AIDS, the strongest risk factor for PEL, in eastern Asia [19]. Given the rarity of HIV/AIDS in eastern Asia, studies of PEL in patients from that area may provide novel insights into the nature of the disease.

Here we describe the clinicopathologic characteristics and outcomes of a series of 17 patients with BCBL in South Korea. The aim of this study was to identify the distinctive clinicopathologic characteristics and outcomes of BCBL in a country with low HIV burden. To our knowledge, this series represents the largest collection of novel cases of HIV-unrelated PEL and the largest Asian BCBL cohort that is independent of the existing literature.

Materials and Methods

1. Case selection

This retrospective study included all consecutive patients diagnosed with BCBL from April 2002 to October 2016 at nine institutions in South Korea. We defined BCBL as NHL that (1) presented exclusively or predominantly as a malignant effusion with or without extra-cavitary lesions, (2) was confirmed either by cytological evaluation of the malignant effusion or by tissue biopsy that revealed cytomorphology characteristic of PEL, and (3) did not meet the diagnostic criteria for any subtype of NHL other than PEL as per the current WHO classification on the basis of the clinical history, cytomorphology, immunophenotype, and genetic alterations (e.g., c-myc gene rearrangement in Burkitt lymphoma). We excluded patients diagnosed with Burkitt or Burkitt-like lymphoma, plasmablastic lymphoma, anaplastic large cell lymphoma, and pyothorax-associated lymphoma (designated as ‘diffuse large B cell lymphoma associated with chronic inflammation’ in the 2016 WHO classification) [3].

2. Data collection

We collected the following data for each patient via medical records review: demographics; sites of involved body cavities and extra-cavitary lesions; performance status; international prognostic index score; medical histories including hepatitis B virus (HBV), hepatitis C virus (HCV), and HIV infections, co-occurring malignancies (e.g., Kaposi’s sarcoma), multicentric Castleman disease, autoimmune disease, and iatrogenic immunosuppression such as organ transplantation, immunosuppressant administration, or cytotoxic chemotherapy; initial laboratory profiles; treatment history; and outcome. We reviewed pathology reports for all of the patients to confirm the diagnosis of BCBL and to obtain immunophenotype information. The HHV8 infection status of lymphoma cells was determined by immunocytochemical staining for latency-associated nuclear antigen 1 (LANA1) using mouse anti-HHV8 monoclonal antibodies (Cell Marq, Hot Springs, AR) and the Ventana BenchMark XT autostainer (Ventana Medical Systems, Tucson, AZ). Coinfection with Epstein-Barr virus (EBV) was assessed by in situ hybridization for EBV-encoded small RNA (EBER).

3. Statistical analysis

We divided the patients into three groups on the basis of the LANA1 immunostaining results—those with PEL (LANA1 positive), those with HHV8-unrelated BCBL (LANA1 negative), and those with HHV8-unknown BCBL (not tested for LANA1 expression)—to describe and compare their clinicopathologic characteristics and prognoses. We summarized the data as medians (ranges) for continuous variables and numbers (%) for discrete variables. We used the International Working Group response criteria to assess the response to treatment [20]. We defined progression-free survival (PFS) as the time from diagnosis to disease progression or death from any cause, whichever occurred first. To estimate PFS, we censored patients who were progression-free and alive at the time of the last assessment of the disease state. We defined overall survival (OS) as the time from diag-nosis to death from any cause. To estimate OS, we censored patients who were alive at the last follow-up date. We used the Kaplan-Meier method to estimate the median PFS and OS. No statistical test was performed because of the small number of cases.

4. Ethical statement

The Institutional Review Board at each participating institution reviewed and approved the study protocol (Seoul National University Hospital Institutional Review Board approval number: H-1511-051-718). Patient consent was waived because of the retrospective nature of the study and lack of patient interaction. We carried out all study procedures including data collection and analyses in accordance with the ethical standards of the Helsinki Declaration (revised in 2013; World Medical Association).

Results

1. Clinical characteristics

We identified a total of 17 BCBL cases. LANA1 immunostaining results were available for 12 patients, of which six were positive for LANA1 (indicating PEL) and six were negative for LANA1 (indicating HHV8-unrelated BCBL). The demographic and clinical characteristics of the patients are described in Table 1 and summarized for the entire cohort and for subgroups based on HHV8 positivity in Table 2. All of the patients were native Koreans of the Asian race. Diagnosis of BCBL was made by cytological evaluation of lymphomatous effusion in 12 patients (70.6%) and by tissue biopsy in the remaining five patients (29.4%)—pleural biopsy in four patients and cervical lymph node biopsy in one patient. At the time of diagnosis, four patients with PEL and one patient with HHV8-unrelated BCBL had B symptoms (fever, weight loss, or night sweats). The male-to-female ratio was 5:1 in both the PEL cohort and the HHV8-unrelated BCBL cohort and 1:4 in the HHV8-unknown BCBL cohort. Except in two patients (one with PEL who had cervical lymph node and bone marrow lesions and one with HHV8-unknown BCBL who had a lung lesion, all of which were pathologically confirmed), BCBL presented exclusively as malignant effusions involving one or multiple body cavities (Tables 1 and 2). The pleural space was the most frequently involved body cavity in all three categories of BCBL. Interestingly, one case of HHV8-unrelated BCBL exclusively involved the vitreous body of the eye (case 7) (Table 1).

Only one of the 17 patients with BCBL had HIV infection (case 3) (Table 1). Except for that patient, no patient had any apparent history of immunocompromising conditions or iatrogenic immunosuppression. One patient with PEL and one patient with HHV8-unknown BCBL were positive for HBV surface antigen. One patient with HHV8-unrelated BCBL was positive for anti-HCV antibody; however, a reverse transcription-polymerase chain reaction assay did not detect HCV RNA, indicating that the HCV infection was resolved. None of the patients had any history of Kaposi’s sarcoma, multicentric Castleman disease, or autoimmune disease. Blood cell counts were unremarkable for most patients; one patient who had HBV-related liver cirrhosis showed moderate thrombocytopenia with a platelet count of 66,000/μL at the time of PEL diagnosis (Table 2).

2. Immunophenotype and EBV status

A representative case of PEL with a characteristic cytomorphology and immunohistochemical staining pattern for LANA1 is shown in Fig. 1. The results of immunophenotypic analysis and in situ hybridization for EBER are shown in Fig. 2. All six patients with PEL had diffuse and strong positivity for LANA1. Three of the six patients (50%) with PEL were positive for EBER, whereas all but one of the patients (83.3%) with HHV8-unrelated BCBL were negative for EBER. Of the five patients with PEL who were tested for CD20 expression, one was weakly positive (20%); that patient also displayed CD79a expression with focal positivity (case 3). In contrast, all six patients with HHV8-unrelated BCBL were positive for CD20 expression. In the reports in the literature, lymphocyte activation markers CD30 and CD38, pan-leukocyte marker CD45, and plasma cell differentiation marker CD138 are all commonly expressed in patients with PEL [16]. Results for CD30, CD38, CD45, and CD138 were positive in four of five (80%), two of two (100%), one of three (33.3%), and four of five (80%) patients with PEL who were tested for those markers in our study, respectively. In contrast, results for CD30 and CD138 were negative in three of three (100%) and two of two (100%) patients with HHV8-unrelated BCBL who were tested for those markers, respectively. Results for multiple myeloma oncogene 1/interferon regulatory factor 4 were positive in six of seven patients tested (85.7%), including two patients with PEL, three patients with HHV8-unrelated BCBL, and one patient with HHV8-unknown BCBL. Results for immunoglobulin κ and λ light chains were negative in all seven of the patients tested. Among the five patients with PEL and four patients with HHV8-unrelated BCBL who were tested for the fraction of Ki-67–positive tumor cells (Ki-67 labeling index), three with PEL had a Ki-67 labeling index ≤ 40% (cases 3 and 4, 30%; case 1, 40%), indicating a low proliferative activity and indolent disease, whereas all four with HHV8-unrelated BCBL had a Ki-67 labeling index ≥ 50% (case 8, 95%; cases 10 and 11, 90%; case 12, 50%). Immunophenotype data were limited for the patients with HHV8-unknown BCBL; CD20 and CD45 were expressed in all two (100%) and three (100%) of the patients with HHV8-unknown BCBL who were tested, respectively.

3. Treatment and response

Fourteen patients were treated with cytotoxic chemotherapy. Three of the patients with PEL did not receive any chemotherapy because of old age (cases 4 and 5) or an unknown reason (case 3). The first-line treatments are summarized in Table 3. Most patients received combination chemotherapy of cyclophosphamide, doxorubicin, vincristine, and prednisolone (CHOP) with (n=5) or without (n=5) rituximab. All three patients with PEL who were treated with chemotherapy were negative for CD20 and received CHOP alone as a first-line treatment. All but one of the patients with HHV8-unrelated BCBL (all positive for CD20) received rituximabcontaining regimens such as rituximab plus CHOP (R-CHOP, n=4) or rituximab plus cyclophosphamide, vincristine, and prednisolone (R-CVP, n=1). The remaining patient with HHV8-unrelated BCBL, who presented with a malignant effusion involving only the vitreous cavity of the eye, was treated with high-dose methotrexate, which resulted in complete remission (CR). The first-line regimens were more heterogeneous in the HHV8-unknown BCBL cohort (Table 3).

We had response information for 12 of the 14 patients that received cytotoxic chemotherapy. Overall, nine patients (75%) achieved CR and three patients (25%) achieved partial remission (PR) after the first-line treatment. R-CHOP resulted in CR in all five of the patients who received it (four with HHV8-unrelated BCBL and one with HHV8-unknown BCBL). CHOP alone resulted in one PR and one CR in the PEL cohort and two CRs in the HHV8-unknown BCBL cohort. Two patients with HHV8-unknown BCBL who were treated with cyclophosphamide, vincristine, and prednisolone (CVP)-based regimens achieved PR (cases 14 and 15). One patient with PEL who received CHOP and achieved CR experienced relapse of the lymphoma at 28 months after initial diagnosis and was subsequently treated with ifosfamide, carboplatin, and etoposide (ICE) combination chemotherapy followed by autologous hematopoietic stem cell transplantation (autoHSCT), which resulted in PR (case 6).

4. Survival

The PFS and OS of each patient are described in Table 1. During a median follow-up period of 1.4 years (range, 0.2 to 14.4 years), one patient with PEL (5.9% overall) experienced disease progression, and three patients (17.6% overall) died, one each with PEL (case 4), HHV8-unrelated BCBL (case 7), and HHV8-unknown BCBL (case 14). The cause of death was unknown for the patient with PEL and pneumonia for the other two patients.

The patient with PEL that showed the lowest tumor cell growth fraction (case 4) survived for > 4 years without any chemotherapy. Another patient with PEL who had HIV infection (case 3) survived for > 1 year without chemotherapy. The patient with PEL that relapsed 28 months after CR lived for > 12 years thereafter with salvage ICE chemotherapy and autoHSCT (case 6). In the PEL, HHV8-unrelated BCBL, and HHV8-unknown BCBL cohorts, the median PFS was 3.2 years, 5.5 years, and not reached, respectively, and the median OS was 4.1 years, 5.5 years, and not reached, respectively.

Discussion

We described 17 patients with BCBL diagnosed in South Korea with a focus on clinicopathologic characteristics and outcomes. We divided the patients into three categories based on the results of immunocytochemical staining for LANA1, which is currently the standard assay to detect HHV8 infection in lymphoma cells, an essential requirement for a diagnosis of PEL [1]. We found a remarkable lack of association between immunocompromising conditions and PEL. Only one of six patients with PEL was HIV seropositive, and no other patient had a prior history of organ transplantation, immunosuppressant treatment, or cytotoxic chemotherapy. Therefore, five of the six patients with PEL in our series were ostensibly immunocompetent. By contrast, more than 90% of patients with PEL described in the literature are HIV seropositive, mostly with severely decreased circulating CD4-positive T cell counts (Table 4) [4-8]. That remarkable difference in the rate of HIV seropositivity among patients with PEL between our series and the patients described in the literature—most of whom are Caucasians from regions with moderate-to-high HIV prevalence—is in line with the fact that South Korea has one of the lowest HIV burdens worldwide, with an HIV seroprevalence of only 0.003%-0.01% [21].

Patients with PEL in our study had a favorable prognosis with a median OS of 4.1 years, whereas those in previous studies consistently had poor outcomes, with a median OS of around 6 months (Table 4) [4-8]. Most patients in the literature with PEL and HIV infection had severe immunodeficiency at the time of PEL diagnosis, with more than 60% having a prior history of AIDS-related illness and a CD4-positive T cell count of < 200/μL [4-8]. Four out of eight deaths in a previous series of patients with HIV infection and PEL were due to causes unrelated to PEL, such as sepsis or hemoptysis [5]. In addition, there have been several reports in which patients with HIV infection and PEL were successfully treated with antiretroviral therapy alone [6,22,23]. In the present cohort, two patients with the lowest Ki-67 labeling index (30%) survived for 14 and 49 months each without having had chemotherapy at the end of follow-up, which is in contrast to the PEL patients in the literature who mostly demonstrated a high proliferation index [24]. Taken together, our findings suggest that the poor outcomes observed in historical PEL cases are largely due to HIV-related complications, such as opportunistic infections and malignancies, rather than to PEL. In that case, watchful waiting with only supportive measures such as drainage of the effusion would be appropriate for the management of chemotherapy-intolerant, HIV-seronegative patients with PEL. In particular, PEL with low proliferation activity might represent an indolent subset that patients can survive for a long time, suggesting the potential value of Ki-67 immunostaining for the prognostic stratification of PEL. Given the small size and the retrospective nature of our case series, future prospective studies are needed to confirm those hypotheses.

Although the detection of HHV8 infection in neoplastic cells is essential to make a diagnosis of PEL as per the WHO criteria [3], we found that five out of 17 patients with BCBL were not tested for HHV8 positivity, and the majority of patients with HHV8-unrelated or HHV8-unknown BCBL were mislabeled as having PEL in their medical records or pathology reports (data not shown). The frequent misdiagnosis of HHV8-unrelated BCBL as PEL is due in part to the lack of a specific subcategory describing HHV8-unrelated BCBL in the current NHL classification despite its unique clinical features [17]. For that reason, there are many different terms for HHV8-unrelated BCBL in the literature, such as HHV8-unrelated PEL-like lymphoma [15,18], HHV8-negative malignant effusion lymphoma [14], and HHV8-negative effusion-based lymphoma [17], causing difficulties in clarifying their clinical entity. Discrimination between PEL and HHV8-unrelated BCBL is crucial, because patients with HHV8-unrelated BCBL have a high expression rate (> 80%) of pan-B-cell markers including CD20, whereas those with PEL do not [16-18], which means that rituximab should have therapeutic value for HHV8-unrelated BCBL but not for PEL. Indeed, in our study, all four patients with HHV8-unrelated BCBL who were treated with R-CHOP achieved CR.

In our study as well as in the literature, patients with PEL, but not those with HHV8-unrelated BCBL, were frequently positive for CD30 expression [7,8,16]. The high rate of CD30 expression in PEL is reminiscent of other CD30-expressing lymphomas such as anaplastic large cell lymphoma, Hodgkin lymphoma, and a subset of diffuse large B-cell lymphoma, for which brentuximab vedotin, an anti-CD30 antibody conjugated to the anti-microtubule agent monomethyl auristatin E, has shown antitumor activity [25-28]. In a recent preclinical study, brentuximab vedotin demonstrated a promising antitumor effect on PEL cell lines and xenograft mice, urging further investigation in clinical trials [29]. The findings of our study suggest that physicians should give more attention to precise discrimination between HHV8-unrelated BCBL and PEL for optimal management and clinical trial design.

Our study has several limitations. First, the small number of cases and the incompleteness of the data preclude drawing firm conclusions in many aspects. For instance, we could not determine whether the tumor cell immunophenotype of PEL differed between immunocompetent patients and immunocompromised patients. Although our results suggest that PEL may have more favorable outcomes in patients without HIV infection than in those with HIV infection, we did not test that hypothesis explicitly, which will require a prospective study with a larger cohort. Second, as this study is a medical record-based retrospective study with no central pathology review conducted, the determination of LANA1 immunostaining positivity was not standardized across participating institutions. Third, most of our analyses were descriptive in nature, with no formal statistical testing. Nonetheless, PEL is so rare that, even worldwide, the largest consecutive series of classic PEL cases included only 34 patients, and that of HHV8-unrelated BCBL cases is even smaller [8,17]. To our knowledge, our series represents the largest collection of consecutive Asian patients with PEL or HHV8-unrelated or HHV8-unknown BCBL.

In conclusion, in a consecutive series of patients with BCBL in a country with low HIV burden, most patients with PEL were HIV seronegative and immunocompetent. Those patients, especially those with low proliferation activity of neoplastic cells, had an indolent disease course and long survival even with supportive care only. Our findings raise a testable hypothesis that watchful waiting is a reasonable approach when managing HIV-seronegative patients with PEL who may not tolerate chemotherapy or whose neoplastic cells show low proliferative activity. The Ki-67 labeling index might serve as a marker for the prognostic stratification of PEL. HHV8-unrelated BCBL was successfully treated with rituximab-containing chemotherapy, which corroborates the findings of previous studies [14-18]. We also found that physicians often fail to distinguish between HHV8-unrelated BCBL and PEL despite the important therapeutic implications. The upcoming revisions of the WHO classification of lymphoid neoplasms should be based on a thorough discussion of the most proper categorization of HHV8-unrelated BCBL. Several emerging hypotheses generated from the present study should be confirmed in future prospective studies based on larger cohorts.

NOTES

Conflict of interest relevant to this article was not reported.

Acknowledgements

This work was supported by a grant from the National Research Foundation of Korea for the Cellular Heterogeneity Research Center (NRF-2016R1A5A1011974), funded by the Ministry of Science, ICT & Future planning, Republic of Korea. The authors thank the Consortium for Improving Survival of Lymphoma for their efforts in coordinating the study. This manuscript has been edited by native English-speaking experts associated with BioScience Writers LLC, Houston, TX, USA.

Fig. 1.

Lymphoma cells with positive nuclear staining for latency-associated nuclear antigen 1 in the ascitic fluid of a representative primary effusion lymphoma patient. (A) Ascites smear exhibits individually scattered tumor cells with an immunoblastic or plasmablastic cytomorphology. (B) Immunohistochemistry using an anti‒human herpesvirus 8 antibody shows dark brown nuclear staining of tumor cells.

Fig. 2.

Immunophenotypic analysis and in situ hybridization for Epstein-Barr virus–encoded small RNA (EBER). The cases are presented in the same order as in Table 1. Latency-associated nuclear antigen 1 (LANA1) and EBER are shown in the first and second columns, respectively, while the remaining markers are arranged in descending order of the number of cases in which they were analyzed. All markers that were stained in at least one case are shown. Blank tiles indicate that assays were not performed or data were not available. MUM1, multiple myeloma oncogene 1; IRF4, interferon regulatory factor 4; κ, immunoglobulin κ light chain; λ, immunoglobulin λ light chain.

Table 1.

Clinical characteristics of 17 patients with body cavity–based lymphoma

Case No.	Sex	Age at diagnosis (yr)	Site of lymphoma involvement at diagnosis	HHV8	ECOG PS	IPI score	Comorbidity	Treatment^a)	Response to chemotherapy	PFS (mo)	OS (mo)
1	M	70	Pleural cavity	Positive	1	1	Lung cancer	1st-line CHOP	PR	≥ 6	≥ 11
2	M	67	Pleural cavity, bone marrow, cervical lymph node	Positive	1	4	HBV	1st-line CHOP	NA	≥ 6	≥ 9
3	M	59	Pleural cavity	Positive	1	1	HIV	No chemotherapy	NA	≥ 4	≥ 14
4	F	78	Pleural cavity	Positive	2	4	None	No chemotherapy	NA	49	49
5	M	87	Pleural cavity	Positive	3	NA	None	No chemotherapy	NA	≥ 5	≥ 6
6	M	60	Pleural cavity, pericardial cavity, peritoneal cavity	Positive	2	3	None	1st-line CHOP	CR followed by PD	28	≥ 173
6	M	60	Pleural cavity, pericardial cavity, peritoneal cavity	Positive	2	3	None	2nd-line ICE → autoHSCT	PR	28	≥ 173
7	F	59	Vitreous body (eyeball)	Negative	0	0	HCV	1st-line HD-MTX	CR	66	66
8	M	80	Pleural cavity, pericardial cavity	Negative	3	5	None	1st-line R-CHOP	CR	≥ 17	≥ 17
9	M	70	Pericardial cavity	Negative	1	3	None	1st-line R-CHOP	CR	≥ 14	≥ 14
10	M	77	Pleural cavity, peritoneal cavity	Negative	2	5	None	1st-line R-CHOP	CR	≥ 12	≥ 12
11	M	83	Pleural cavity	Negative	2	4	None	1st-line R-CVP	NA	≥ 2	≥ 2
12	M	75	Pleural cavity, pericardial cavity	Negative	1	3	None	1st-line R-CHOP	CR	≥ 46	≥ 49
13	F	76	Pleural cavity	Unknown	2	2	None	1st-line CHOP	CR	≥ 5	≥ 5
14	F	73	Pleural cavity, peritoneal cavity	Unknown	1	4	HBV	1st-line CVP	PR	10	10
15	M	86	Pleural cavity	Unknown	1	4	None	1st-line R-CVP	PR	≥ 24	≥ 24
16	F	66	Pleural cavity, peritoneal cavity	Unknown	1	4	None	1st-line CHOP	CR	≥ 99	≥ 99
17	F	39	Pleural cavity, lung	Unknown	1	3	None	1st-line R-CHOP	CR	≥ 123	≥ 123

HHV8, human herpesvirus 8; ECOG PS, Eastern Cooperative Oncology Group performance status; IPI, international prognostic index; PFS, progression-free survival; OS, overall survival; CHOP, cyclophosphamide, doxorubicin, vincristine, and prednisolone; PR, partial remission; HBV, hepatitis B virus; NA, not assessed or not available; HIV, human immunodeficiency virus; ICE, ifosfamide, carboplatin, and etoposide; autoHSCT, autologous hematopoietic stem cell transplantation; CR, complete remission; PD, progressive disease; HCV, hepatitis C virus; HD-MTX, high-dose methotrexate; R-CHOP, rituximab plus CHOP; CVP, cyclophosphamide, vincristine, and prednisolone; R-CVP, rituximab plus CVP.

^a) Drainage was performed if clinically indicated.

Table 2.

Patient characteristics of the entire cohort and subgroups based on HHV8 positivity^a)

Characteristic	Entire cohort (n=17)	PEL (n=6)	HHV8-unrelated BCBL (n=6)	HHV8-unknown BCBL (n=5)
Age at diagnosis (yr)	73 (39-87)	68.5 (59-87)	76 (59-83)	73 (39-86)
Sex
Male	11/17 (64.7)	5/6 (83.3)	5/6 (83.3)	1/5 (20.0)
Female	6/17 (35.3)	1/6 (16.7)	1/6 (16.7)	4/5 (80.0)
Site of lymphoma involvement
Pleural cavity	15/17 (88.2)	6/6 (100)	4/6 (66.7)	5/5 (100)
Pericardial cavity	4/17 (23.5)	1/6 (16.7)	3/6 (50.0)	0/5 (0)
Peritoneal cavity	4/17 (23.5)	1/6 (16.7)	1/6 (16.7)	2/5 (40.0)
Extra-cavitary lesion^b)	2/17 (11.8)	1/6 (16.7)	0/6 (0)	1/5 (20.0)
ECOG PS at diagnosis
0-1	10/17 (58.8)	3/6 (50.0)	3/6 (50.0)	4/5 (80.0)
2-4	7/17 (41.2)	3/6 (50.0)	3/6 (50.0)	1/5 (20.0)
IPI risk group
Low (0-1)	3/16 (18.8)	2/5 (40.0)	1/6 (16.7)	0/5 (0)
Low-intermediate (2)	1/16 (6.2)	0/5 (0)	0/6 (0)	1/5 (20.0)
High-intermediate (3)	4/16 (25.0)	1/5 (20.0)	2/6 (33.3)	1/5 (20.0)
High (4-5)	8/16 (50.0)	2/5 (40.0)	3/6 (50.0)	3/5 (60.0)
Comorbidity
HIV	1/17 (5.9)	1/6 (16.7)	0/6 (0)	0/5 (0)
HBV	2/17 (11.8)	1/6 (16.7)	0/6 (0)	1/5 (20.0)
HCV	1/17 (5.9)	0/6 (0)	1/6 (16.7)	0/5 (0)
Co-occurring malignancy	1/17 (5.9)^c)	1/6 (16.7)^c)	0/6 (0)	0/5 (0)
Laboratory profile at diagnosis
WBC ≥ 10,000/μL	3/17 (17.6)	1/6 (16.7)	0/6 (0)	2/5 (40.0)
Hemoglobin < 10 g/dL	4/17 (23.5)	1/6 (16.7)	2/6 (33.3)	1/5 (20.0)
Platelet < (130×10³)/μL	1/17 (5.9)	1/6 (16.7)	0/6 (0)	0/5 (0)
Serum albumin < 3.5 g/dL	12/17 (70.6)	5/6 (83.3)	5/6 (83.3)	2/5 (40.0)
Serum creatinine ≥ 1.5 mg/dL	3/17 (17.6)	1/6 (16.7)	1/6 (16.7)	1/5 (20.0)
LDH above normal	13/16 (81.2)	4/6 (66.7)	4/5 (80.0)	5/5 (100)
β2-microglobulin ≥ 3.5 mg/dL	5/10 (50.0)	2/4 (50.0)	2/5 (40.0)	1/1 (100)
C-reactive protein ≥ 0.8 mg/dL	17/17 (100)	6/6 (100)	6/6 (100)	5/5 (100)

Values are presented as median (range) or number (%). HHV8, human herpesvirus 8; PEL, primary effusion lymphoma; BCBL, body cavity-based lymphoma; ECOG PS, Eastern Cooperative Oncology Group performance status; IPI, international prognostic index; HIV, human immunodeficiency virus; HBV, hepatitis B virus; HCV, hepatitis C virus; WBC, white blood cell; LDH, lactate dehydrogenase.

^a) The proportions are calculated as the frequency divided by the total number of evaluable patients for each characteristic,

^b) Extra-cavitary lesions were present in one patient with PEL who had both bone-marrow and cervical lymph-node lesions and one patient with HHV8-unknown BCBL who had a lung lesion,

^c) Lung cancer.

Table 3.

First-line chemotherapy regimens

Regimen	Entire cohort (n=17)	PEL (n=6)	HHV8-unrelated BCBL (n=6)	HHV8-unknown BCBL (n=5)
CHOP	5 (29.4)	3 (50.0)	0	2 (40.0)
R-CHOP	5 (29.4)	0	4 (66.7)	1 (20.0)
CVP	1 (5.9)	0	0	1 (20.0)
R-CVP	2 (11.8)	0	1 (16.7)	1 (20.0)
HD-MTX	1 (5.9)	0	1 (16.7)	0
No chemotherapy	3 (17.6)	3 (50.0)	0	0

Values are presented as number (%). PEL, primary effusion lymphoma; HHV8, human herpesvirus 8; BCBL, body cavity-based lymphoma; CHOP, cyclophosphamide, doxorubicin, vincristine, and prednisolone; R-CHOP, rituximab plus CHOP; CVP, cyclophosphamide, vincristine, and prednisolone; R-CVP, rituximab plus CVP; HD-MTX, high-dose methotrexate.

Table 4.

Comparison of clinical characteristics and outcome of classic PEL cases between the historical controls^a) and the present cohort

Characteristic	Nador et al. [4]	Boulanger et al. [5]	Simonelli et al. [6]	Boulanger et al. [7]	Guillet et al. [8]	Present cohort
No. of cases	15	12	11	28	34	6
Male-to-female ratio	15:0	12:0	10:1	27:1	31:3	5:1
Age, median (range, yr)	44 (31-85)	43.5 (33-66)	41 (26-58)	44 (33-78)	45 (40-54)	68.5 (59-87)
HIV positivity rate (%)	87	100	100	100	100	17
Median survival	5 mo	5.6 mo	6 mo	6.2 mo	10.2 mo	4.1 yr

PEL, primary effusion lymphoma; HIV, human immunodeficiency virus.

^a) The five largest case series of PEL reported thus far (to our knowledge) were used for comparison.

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Figure & Data

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Body Cavity–Based Lymphoma in a Country with Low Human Immunodeficiency Virus Prevalence: A Series of 17 Cases from the Consortium for Improving Survival of Lymphoma

Cancer Res Treat. 2019;51(4):1302-1312. Published online February 14, 2019

DOI: https://doi.org/10.4143/crt.2018.555

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Abstract
Introduction
Materials and Methods
Results
Discussion
NOTES
REFERENCES

Body Cavity–Based Lymphoma in a Country with Low Human Immunodeficiency Virus Prevalence: A Series of 17 Cases from the Consortium for Improving Survival of Lymphoma

Fig. 1. Lymphoma cells with positive nuclear staining for latency-associated nuclear antigen 1 in the ascitic fluid of a representative primary effusion lymphoma patient. (A) Ascites smear exhibits individually scattered tumor cells with an immunoblastic or plasmablastic cytomorphology. (B) Immunohistochemistry using an anti‒human herpesvirus 8 antibody shows dark brown nuclear staining of tumor cells.

Fig. 2. Immunophenotypic analysis and in situ hybridization for Epstein-Barr virus–encoded small RNA (EBER). The cases are presented in the same order as in Table 1. Latency-associated nuclear antigen 1 (LANA1) and EBER are shown in the first and second columns, respectively, while the remaining markers are arranged in descending order of the number of cases in which they were analyzed. All markers that were stained in at least one case are shown. Blank tiles indicate that assays were not performed or data were not available. MUM1, multiple myeloma oncogene 1; IRF4, interferon regulatory factor 4; κ, immunoglobulin κ light chain; λ, immunoglobulin λ light chain.

Fig. 1.

Fig. 2.

Body Cavity–Based Lymphoma in a Country with Low Human Immunodeficiency Virus Prevalence: A Series of 17 Cases from the Consortium for Improving Survival of Lymphoma

Case No.	Sex	Age at diagnosis (yr)	Site of lymphoma involvement at diagnosis	HHV8	ECOG PS	IPI score	Comorbidity	Treatment^a)	Response to chemotherapy	PFS (mo)	OS (mo)
1	M	70	Pleural cavity	Positive	1	1	Lung cancer	1st-line CHOP	PR	≥ 6	≥ 11
2	M	67	Pleural cavity, bone marrow, cervical lymph node	Positive	1	4	HBV	1st-line CHOP	NA	≥ 6	≥ 9
3	M	59	Pleural cavity	Positive	1	1	HIV	No chemotherapy	NA	≥ 4	≥ 14
4	F	78	Pleural cavity	Positive	2	4	None	No chemotherapy	NA	49	49
5	M	87	Pleural cavity	Positive	3	NA	None	No chemotherapy	NA	≥ 5	≥ 6
6	M	60	Pleural cavity, pericardial cavity, peritoneal cavity	Positive	2	3	None	1st-line CHOP	CR followed by PD	28	≥ 173
6	M	60	Pleural cavity, pericardial cavity, peritoneal cavity	Positive	2	3	None	2nd-line ICE → autoHSCT	PR	28	≥ 173
7	F	59	Vitreous body (eyeball)	Negative	0	0	HCV	1st-line HD-MTX	CR	66	66
8	M	80	Pleural cavity, pericardial cavity	Negative	3	5	None	1st-line R-CHOP	CR	≥ 17	≥ 17
9	M	70	Pericardial cavity	Negative	1	3	None	1st-line R-CHOP	CR	≥ 14	≥ 14
10	M	77	Pleural cavity, peritoneal cavity	Negative	2	5	None	1st-line R-CHOP	CR	≥ 12	≥ 12
11	M	83	Pleural cavity	Negative	2	4	None	1st-line R-CVP	NA	≥ 2	≥ 2
12	M	75	Pleural cavity, pericardial cavity	Negative	1	3	None	1st-line R-CHOP	CR	≥ 46	≥ 49
13	F	76	Pleural cavity	Unknown	2	2	None	1st-line CHOP	CR	≥ 5	≥ 5
14	F	73	Pleural cavity, peritoneal cavity	Unknown	1	4	HBV	1st-line CVP	PR	10	10
15	M	86	Pleural cavity	Unknown	1	4	None	1st-line R-CVP	PR	≥ 24	≥ 24
16	F	66	Pleural cavity, peritoneal cavity	Unknown	1	4	None	1st-line CHOP	CR	≥ 99	≥ 99
17	F	39	Pleural cavity, lung	Unknown	1	3	None	1st-line R-CHOP	CR	≥ 123	≥ 123

Characteristic	Entire cohort (n=17)	PEL (n=6)	HHV8-unrelated BCBL (n=6)	HHV8-unknown BCBL (n=5)
Age at diagnosis (yr)	73 (39-87)	68.5 (59-87)	76 (59-83)	73 (39-86)
Sex
Male	11/17 (64.7)	5/6 (83.3)	5/6 (83.3)	1/5 (20.0)
Female	6/17 (35.3)	1/6 (16.7)	1/6 (16.7)	4/5 (80.0)
Site of lymphoma involvement
Pleural cavity	15/17 (88.2)	6/6 (100)	4/6 (66.7)	5/5 (100)
Pericardial cavity	4/17 (23.5)	1/6 (16.7)	3/6 (50.0)	0/5 (0)
Peritoneal cavity	4/17 (23.5)	1/6 (16.7)	1/6 (16.7)	2/5 (40.0)
Extra-cavitary lesion^b)	2/17 (11.8)	1/6 (16.7)	0/6 (0)	1/5 (20.0)
ECOG PS at diagnosis
0-1	10/17 (58.8)	3/6 (50.0)	3/6 (50.0)	4/5 (80.0)
2-4	7/17 (41.2)	3/6 (50.0)	3/6 (50.0)	1/5 (20.0)
IPI risk group
Low (0-1)	3/16 (18.8)	2/5 (40.0)	1/6 (16.7)	0/5 (0)
Low-intermediate (2)	1/16 (6.2)	0/5 (0)	0/6 (0)	1/5 (20.0)
High-intermediate (3)	4/16 (25.0)	1/5 (20.0)	2/6 (33.3)	1/5 (20.0)
High (4-5)	8/16 (50.0)	2/5 (40.0)	3/6 (50.0)	3/5 (60.0)
Comorbidity
HIV	1/17 (5.9)	1/6 (16.7)	0/6 (0)	0/5 (0)
HBV	2/17 (11.8)	1/6 (16.7)	0/6 (0)	1/5 (20.0)
HCV	1/17 (5.9)	0/6 (0)	1/6 (16.7)	0/5 (0)
Co-occurring malignancy	1/17 (5.9)^c)	1/6 (16.7)^c)	0/6 (0)	0/5 (0)
Laboratory profile at diagnosis
WBC ≥ 10,000/μL	3/17 (17.6)	1/6 (16.7)	0/6 (0)	2/5 (40.0)
Hemoglobin < 10 g/dL	4/17 (23.5)	1/6 (16.7)	2/6 (33.3)	1/5 (20.0)
Platelet < (130×10³)/μL	1/17 (5.9)	1/6 (16.7)	0/6 (0)	0/5 (0)
Serum albumin < 3.5 g/dL	12/17 (70.6)	5/6 (83.3)	5/6 (83.3)	2/5 (40.0)
Serum creatinine ≥ 1.5 mg/dL	3/17 (17.6)	1/6 (16.7)	1/6 (16.7)	1/5 (20.0)
LDH above normal	13/16 (81.2)	4/6 (66.7)	4/5 (80.0)	5/5 (100)
β2-microglobulin ≥ 3.5 mg/dL	5/10 (50.0)	2/4 (50.0)	2/5 (40.0)	1/1 (100)
C-reactive protein ≥ 0.8 mg/dL	17/17 (100)	6/6 (100)	6/6 (100)	5/5 (100)

Regimen	Entire cohort (n=17)	PEL (n=6)	HHV8-unrelated BCBL (n=6)	HHV8-unknown BCBL (n=5)
CHOP	5 (29.4)	3 (50.0)	0	2 (40.0)
R-CHOP	5 (29.4)	0	4 (66.7)	1 (20.0)
CVP	1 (5.9)	0	0	1 (20.0)
R-CVP	2 (11.8)	0	1 (16.7)	1 (20.0)
HD-MTX	1 (5.9)	0	1 (16.7)	0
No chemotherapy	3 (17.6)	3 (50.0)	0	0

Characteristic	Nador et al. [4]	Boulanger et al. [5]	Simonelli et al. [6]	Boulanger et al. [7]	Guillet et al. [8]	Present cohort
No. of cases	15	12	11	28	34	6
Male-to-female ratio	15:0	12:0	10:1	27:1	31:3	5:1
Age, median (range, yr)	44 (31-85)	43.5 (33-66)	41 (26-58)	44 (33-78)	45 (40-54)	68.5 (59-87)
HIV positivity rate (%)	87	100	100	100	100	17
Median survival	5 mo	5.6 mo	6 mo	6.2 mo	10.2 mo	4.1 yr

Table 1. Clinical characteristics of 17 patients with body cavity–based lymphoma

Drainage was performed if clinically indicated.

Table 2. Patient characteristics of the entire cohort and subgroups based on HHV8 positivitya)

The proportions are calculated as the frequency divided by the total number of evaluable patients for each characteristic,

Extra-cavitary lesions were present in one patient with PEL who had both bone-marrow and cervical lymph-node lesions and one patient with HHV8-unknown BCBL who had a lung lesion,

Lung cancer.

Table 3. First-line chemotherapy regimens

Table 4. Comparison of clinical characteristics and outcome of classic PEL cases between the historical controlsa) and the present cohort

PEL, primary effusion lymphoma; HIV, human immunodeficiency virus.

The five largest case series of PEL reported thus far (to our knowledge) were used for comparison.