Comparison of Clinical Outcomes of BRCA1/2 Pathologic Mutation, Variants of Unknown Significance, or Wild Type Epithelial Ovarian Cancer Patients

Kyung Jin Eoh; Hyung Seok Park; Ji Soo Park; Seung-Tae Lee; Jeongwoo Han; Jung-Yun Lee; Sang Wun Kim; Sunghoon Kim; Young Tae Kim; Eun Ji Nam

doi:10.4143/crt.2016.135

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Original Article Comparison of Clinical Outcomes of BRCA1/2 Pathologic Mutation, Variants of Unknown Significance, or Wild Type Epithelial Ovarian Cancer Patients: Kyung Jin Eoh, MD¹, Hyung Seok Park, MD^2,3, Ji Soo Park, MD, PhD^2,4, Seung-Tae Lee, MD, PhD^2,5, Jeongwoo Han, MD, PhD^2,6, Jung-Yun Lee, MD, PhD¹, Sang Wun Kim, MD, PhD¹, Sunghoon Kim, MD, PhD¹, Young Tae Kim, MD, PhD¹, Eun Ji Nam, MD, PhD^1,2,; Cancer Research and Treatment : Official Journal of Korean Cancer Association 2017;49(2):408-415.
DOI: https://doi.org/10.4143/crt.2016.135
Published online: July 27, 2016

¹Department of Obstetrics and Gynecology, Institute of Women’s Life Medical Science, Women’s Cancer Clinic, Yonsei University College of Medicine, Seoul, Korea

²Hereditary Cancer Clinic of Cancer Prevention Center, Yonsei Cancer Center, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea

³Department of Surgery, Yonsei University College of Medicine, Seoul, Korea

⁴Cancer Prevention Center, Yonsei Cancer Center, Seoul, Korea

⁵Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea

⁶Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea

Correspondence: Eun Ji Nam, MD, PhD Department of Obstetrics and Gynecology, Institute of Women’s Life Medical Science, Women’s Cancer Clinic, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Korea
Tel: 82-2-2228-2250 Fax: 82-2-313-8350 E-mail: NAHMEJ6@yuhs.ac

• Received: March 29, 2016 • Accepted: July 8, 2016

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Abstract

Purpose
The purpose of this study was to investigate the clinical features of epithelial ovarian cancer (EOC) patients according to BRCA1/2 mutation status (mutation, variant of uncertain significance [VUS], or wild type).
Materials and Methods
We analyzed 116 patients whose BRCA1/2 genetic test results were available for mutation type and clinical features, including progression-free survival (PFS), overall survival (OS), and response rate. These characteristics were compared according to BRCA1/2 mutation status.
Results
Thirty-seven (37/116, 31.9%) BRCA1/2mutations were identified (BRCA1, 30; BRCA2, 7). Mutation of c.3627_3628insA (p.Leu1209_Glu1210?fs) in BRCA1 was observed in five patients (5/37, 13.5%). Twenty-five patients had BRCA1/2 VUSs (25/116, 21.6%). Personal histories of breast cancer were observed in 48.6% of patients with BRCA1/2 mutation (18/37), 16.0% of patients with BRCA1/2 VUS (4/25), and 7.4% of patients with BRCA wild type (4/54) (p < 0.001). Patients with BRCA1/2 mutation showed longer OS than those with BRCA1/2 wild type (p=0.005). No significant differences were detected in PFS, OS, or response rates between patients with BRCA1/2 VUS and BRCA1/2 mutation (p=0.772, p=0.459, and p=0.898, respectively).
Conclusion
Patientswith BRCA1/2 mutation had longer OS than thosewith BRCA1/2wild type. Patients with BRCA1/2 mutation and BRCA1/2 VUS displayed similar prognoses.
Key words: Ovarian epithelial cancer, BRCA1, BRCA2, Prognosis

Introduction

Epit helial ovarian cancer (EOC) remains the gynecological cancer with the highest mortality because over two-thirds of patients have advanced disease upon diagnosis [1]. Only 10% of cases are due to an inherited predisposition, while the majority are attributable to alterations in BRCA1 and BRCA2, which present the strongest risk factors for breast and ovarian cancers [2]. Indeed, studies of lifetime risk of ovarian cancer have revealed that it ranges from 39% to 54% in women identified as having a BRCA1/2 mutation, with lower risk associated with BRCA2 mutation than BRCA1 [3].

Most patients with BRCA1/2 mutations have a demonstrably more favorable outcome than those with sporadic ovarian cancers [4,5]. However, the mechanism of this purported survival advantage conferred by BRCA1/2 mutation is not entirely clear. Some have speculated that it results from a greater susceptibility to chemotherapy owing to a significantly higher growth fraction in BRCA1/2-associated malignancies [6]. However, others have not been able to confirm the significant survival advantage conferred by BRCA1/2 mutations, specifically in terms of long term overall survival (OS) [7,8]. Indeed, poorer survival for patients with BRCA1/2 mutations has been reported [9,10], although it remains unclear why different investigators have noted such varying effects of BRCA1/2 alterations on survival.

Several previous studies of BRCA1/2 and EOC have been conducted in Korea, and BRCA1/2 mutations were found in 33% of patients with a strong family history of EOC [11,12]. Possible candidates of a founder mutation in Korea have also been reported [13]. Moreover, a large, prospective, nationwide study of Korean breast cancer patients and their BRCA1/2 status suggested that BRCA1 mutation has a significant negative impact on survival [14]. However, only a few studies have analyzed the effects of BRCA1/2 mutation status on the clinical prognosis of EOC patients of Asian ethnicity [15]. Furthermore, clinical features of EOC patients who have a BRCA1/2 variant of uncertain significance (VUS), which is a gene mutation that has an unknown effect on protein function, have not been reported to date [16]. The potential for an alternate interpretation of a BRCA1/2 VUS over time carries with it possible disparate clinical implications. Thus, characterization of patients with BRCA1/2 VUS is required for counseling and follow-up.

Therefore, this study was conducted to investigate clinical features of EOC patients according to BRCA1/2 mutation status (BRCA1/2 mutation vs. BRCA1/2 VUS vs. BRCA1/2 wild type), including survival.

Materials and Methods

1. Patient selection and pathologic review

The study protocol was approved by the Institutional Review Board. During the review of medical records, we obtained data describing several patient characteristics, including age at diagnosis, histologic type, and surgical stage as classified by the International Federation of Gynecology and Obstetrics criteria.

A patient selection diagram is shown in Fig. 1. A total of 711 patients were pathologically confirmed to have EOC from January 1999 to May 2015. Of these, 595 had uninformative BRCA1/2 mutation statuses and were therefore excluded. A BRCA1/2 genetic test was performed for the remaining 116 patients. A gynecologic oncology team at a single institute conducted all procedures, and a dedicated radiologist at the same institute reviewed all data from imaging studies (e.g., magnetic resonance imaging and computed tomography). From 2011, neoadjuvant chemotherapy followed by interval debulking surgery was introduced in our institution, and 39 out of the 116 patients received this treatment. All 116 patients were treated with platinum based chemotherapy. These patients were analyzed for mutation type and clinical features including family history, personal breast cancer history, progression-free survival (PFS), OS, and response rate.

The response rate was determined using the Response Evaluation Criteria in Solid Tumors system. Specifically, we analyzed PFS and OS in patients with BRCA1/2 mutation and compared these data with those of patients with BRCA1/2 wild type and VUS. PFS was defined as the period in months between the dates of diagnosis and relapse or last contact. OS was defined as the period in months between the dates of diagnosis and death or last contact.

2. Direct sequencing

Genetic testing for BRCA1 and BRCA2 (accession numbers NM_007294 and NM_000059, respectively) mutations was performed using direct sequencing as previously described [12]. The genetic mutations analyzed were confined to deleterious mutations such as frameshift or nonsense mutations. Variations were described following the nomenclature system of the Human Genome Variation Society (http://www.hgvs.org/mutnomen) and the conventional nomenclature system from the Breast Cancer Information Core (BIC; http://research.nhgri.nih.gov/bic/).

3. Statistical analysis

Statistical analysis was performed using IBM SPSS ver. 20.0 (IBM Corp., Armonk, NY). A Kolmogorov-Smirnov test was used to verify standard normal distributional assumptions. Patient clinical features including response rate, PFS, and OS were analyzed using an ANOVA test, a Pearson’s chi-squared test, and Kaplan-Meier survival analysis. A p-value of less than 0.05 was regarded as statistically significant.

Results

1. Patient characteristics

Patient characteristics are shown in Table 1. Among the 116 EOC patients who underwent BRCA1/2 gene tests by the polymerase chain reaction–denaturing high performance liquid chromatography–sequencing method, 37 (37/116, 31.9%) BRCA1/2 mutations were identified (BRCA1, 30; BRCA2, 7). In addition, 25 patients with BRCA1/2 VUSs were identified (25/116, 21.6%) and two different types of BRCA1/2 VUS were found simultaneously in one patient.

No significant differences were detected in terms of mean age, International Federation of Gynecology and Obstetrics stage, cancer histology, grade, and performance of optimal surgery. Personal histories of breast cancer were observed in 48.6% of patients with BRCA1/2 mutation (18/37), 16.0% of patients with BRCA1/2 VUS (4/25), and 7.4% of those with wild type (4/54) (p < 0.001). A family history of BRCA1/2-associated cancer was present in 48.6% of patients with BRCA1/2 mutation (18/37), 36.0% of those with BRCA1/2 VUS (9/25), and 37% of BRCA1/2 patients with BRCA wild type (20/54) (p=0.299).

2. Response rates and survival

The response rate was 83.8% in patients with BRCA1/2 mutation (31/37), 88.0% in patients with BRCA1/2 VUS (22/25), and 85.2% in patients with BRCA1/2 wild type (46/54). No significant differences were detected between the three groups (p=0.898) (Table 2).

The median PFS was 17, 14, and 13 months for patients with BRCA1/2 mutation, VUS, and wild type, respectively. Patients with BRCA1/2 mutation had longer PFS than those with BRCA1/2 wild type, although this difference did not achieve statistical significance (p=0.071). No significant differences were detected in terms of PFS between patients with BRCA1/2 VUS and BRCA1/2 mutation or wild type (p=0.772 and p=0.455, respectively) (Fig. 2).

The median OS was 33, 24, and 17 months in patients with BRCA1/2 mutation, VUS, and wild type, respectively. Patients with BRCA1/2 mutation showed longer OS than those with BRCA1/2 wild type (p=0.005). No significant differences were detected in OS between patients with BRCA1/2 VUS and BRCA1/2 mutation or wild type (p=0.459 and p=0.211, respectively) (Fig. 3).

3. Frequently observed BRCA1/2 alterations

Frequently observed BRCA1/2 alterations in this study are presented in Tables 3 and 4. The c.3627_3628insA (p.Leu1209 _Glu1210?fs) alteration in BRCA1 (exon 11) was recurrent in five patients (5/37, 13.5%). Among the 25 patients with BRCA1/2 VUS, c.8187G>T (p.Lys2729Asn) mutation in BRCA2 (exon 18) was present in four (4/25, 16%). All of the frequently observed alterations were reported in the Breast Cancer Information Core database.

Discussion

In the present study, we investigated the impact of BRCA1/2 mutation status on the clinical features of EOC patients. Patients with BRCA1/2 mutation had improved OS as compared to those with BRCA1/2 wild type. However, no significant differences in PFS and response rates were detected between the groups included in this study. BRCA1/2 mutation and VUS patients had similar prognoses. Greater sensitivity to platinum-based chemotherapy among patients with BRCA1/2 mutation was not identified in this study. To the best of our knowledge, this is the first study that compared the clinical outcomes of EOC patients with BRCA1/2 mutation, VUS, and wild type.

A favorable prognosis for patients with BRCA1/2 mutation over that of patients with BRCA1/2 wild type was identified in this study, although no significant difference was detected in PFS. The underlying mechanism of BRCA1/2 mutation conferring a favorable prognosis remains unclear. The main function of BRCA1/2 proteins is to promote DNA double-strand break repair via homologous recombination. BRCA1 has been implicated in many cellular functions, including DNA repair, the maintenance of genomic integrity, and cell cycle checkpoint control [17,18]. The main function of BRCA2 appears to involve interaction with RAD51 during homologous recombination DNA repair [19]. Cells with mutated BRCA1/2 proteins may therefore be rendered less capable of repairing chemotherapy-induced DNA damage, potentially leading to an improved response to treatment. This is known as 'synthetic lethality,' i.e., the enhanced lethality of DNA-damaging agents.

Our data failed to show significantly longer PFS or a better response rate to chemotherapy in patients with BRCA1/2 mutation over those of patients with BRCA1/2 VUS or wild type. To explain the discrepancy between our data and those of prior studies, a more complex model is necessary to clarify specific mechanisms of BRCA1/2 dysfunction that result in better outcomes for EOC patients. It is estimated that approximately 50% of sporadic EOCs show BRCA1 or BRCA2 dysfunction through different mechanisms. Tumors that share molecular features of BRCA1/2-mutant tumors (i.e., BRCAness) could also emerge in this process that affects or is affected by normal BRCA1/2 gene function [20,21]. Previous studies reported that low BRCA1 or BRCA2 expression in sporadic EOC could confer similar effects on prognosis as BRCA1 or BRCA2 mutation. Specifically, low BRCA1 expression measured by reverse transcription polymerase chain reaction was shown to be a positive prognostic factor for both OS and PFS in patients with sporadic EOCs [22]. This finding indicates that low BRCA1 expression status in sporadic EOC has a similar impact on prognosis as germline BRCA1/2 mutation. Therefore, if “BRCAness” could be measured quantitatively in our study, a precise comparison between “BRCAness” positive and negative groups would possible.

Interpretation of the clinical implications of BRCA1/2 VUS remains challenging because misperception by a physician regarding the implications of VUS could lead to inappropriate risk-reducing surgery, neglect, or providing misinformation to patients. Myriad Genetic Laboratories (Salt Lake City, UT) reported that about 7% of their molecular diagnoses of hereditary breast and ovarian cancer are linked to VUSs [23,24], and these alterations have been identified more commonly in African-American than in Hispanic populations [25,26]. However, few reports have analyzed VUS prevalence in Asian patients. Additionally, limited information is available regarding the clinical features of EOC patients with BRCA1/2 VUS.

Despite the possibility of selection bias, 21.6% of patients (25/116) who underwent BRCA1/2 genetic tests were found to have BRCA1/2 VUS. Among patients with BRCA1/2 VUS, 16% (4/25) had a personal history of double primary breast cancer and 36% (9/25) had a family history of breast or ovarian cancer in first-degree relatives. One of the VUSs found in this study (BRCA1 c.5339T>C) is highly suspected to be a deleterious mutation based on the patients’ family histories of BRCA1/2-associated cancer, personal histories of breast cancer, and population frequency.

Identification of founder mutations is required to improve the quality of genetic counseling. Moreover, using a more specific approach to molecular testing leads to greater cost-effectiveness. If we can recognize differences in susceptibility due to a specific founder mutation, it will be possible to define the role of risk reducing surgery. Frequently observed alterations in this study are presented in Tables 3 and 4. Studies to identify founder mutations have been conducted in Asian countries [27], and one report analyzed possible candidates of a founder mutation in Korea [13]. However, specific mutations that account for a high frequency of cases, such as that observed in the Ashkenazi Jewish population, have not yet been discovered. The frequently observed BRCA1/2 alterations found in this study were not identified in a previous study conducted in Korea. Accordingly, an investigation including a larger number of cases must be analyzed to provide accurate information regarding the frequency of founder mutations.

It should be noted that this study had several limitations. Specifically, it included a small number of patients with BRCA1/2 mutations because of the low rate of genetic testing, had a retrospective design with the possibility of selection bias, and a short follow-up period. Moreover, 595 patients declined BRCA1/2 genetic testing. Conversely, a previous report showed few barriers to participating in genetic counseling and BRCA1/2 testing in Western countries, with a testing rate of 81% [28]. The low rate of genetic testing (16.3%, 116/711) in the present study may be the result of low public awareness regarding its availability. In addition, this study included patients representative of a cancer center population, and all study participants had been affected by EOC. Thus, our findings may not apply to patients who have not had cancer and are undergoing genetic testing due to family history alone.

Routine tests for BRCA1/2 germline mutation status in patients with EOC may be warranted, as it has been demonstrated that a deficiency in the BRCA1/2 gene confers substantial sensitivity to a chemotherapeutic agent, namely poly(ADP-ribose) polymerase-1 (PARP) inhibitor (olaparib) [29]. Further research is required to determine whether application of this agent to EOCs with pathologic BRCA1/2 VUS is beneficial or not. Some patients with BRCA1/2 VUS may be responsive to treatment with PARP inhibitors, which results in synthetic lethality of cells that have deficient homologous recombination or double-strand DNA repair. This might improve survival among such patients. Further prospective cohort studies with longer follow-up periods as well as “BRCAness” quantification are needed to enable a precise understanding of the role of BRCAness on the clinical features of EOC patients.

Conclusion

Our study provides useful data for counseling EOC patients with BRCA1/2 mutation, VUS, and wild type. Patients with BRCA1/2 mutation had more favorable prognosis, or significantly longer OS than those with BRCA1/2 wild type, while they have similar prognoses as patients with BRCA1/2 VUS.

NOTES

Conflict of interest relevant to this article was not reported.

Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2014R1A1A1A05002926; 2015R1A2A2A01008162; 2015R1C1A2A01053516).

Fig. 1.

Patient selection diagram. VUS, variant of uncertain significance.

Fig. 2.

Progression-free survival (PFS) curves according to BRCA1/2 mutation status. Median PFS was 17 months for BRCA1/2 mutation patients, 14 months for BRCA1/2 variant of uncertain significance (VUS) patients, and 13 months for BRCA1/2 wild type patients. A log-rank test revealed longer PFS for BRCA1/2mutation than wild type patients; however, this was not statistically significant (p=0.071). No differences were detected between BRCA1/2 wild type and VUS (p=0.455) or BRCA1/2 mutation and VUS (p=0.772).

Fig. 3.

Overall survival (OS) curves according to BRCA1/2 mutation status. Median OS was 33 months for BRCA1/2 mutation patients, 24 months for BRCA1/2 variant of uncertain significance (VUS) patients, and 17 months for BRCA1/2 wild type patients. A log-rank test revealed significantly longer OS for BRCA1/2mutation than wild type patients (p=0.005). No differences were detected between BRCA1/2 wild type and VUS (p=0.211) or BRCA1/2 mutation and VUS (p=0.459).

Table 1.

Patient characteristics

Characteristic	Overall population (n=116)	BRCA1/2 mutation (n=37)	BRCA1/2 VUS (n=25)	BRCA1/2 wild type (n=54)	p-value
Age
Mean±SD	52.2±11.4	52.4±9.3	52.0±11.6	52.2±12.8	0.99^a)
Stage FIGO
I-II	21 (18.1)	2 (5.4)	6 (24.0)	13 (24.1)	0.059^b)
III-IV	94 (81.0)	34 (91.9)	19 (76.0)	41 (75.9)
NI	1 (0.9)	1 (2.7)	0	0
Histology
Serous	91 (78.4)	32 (86.5)	18 (72.0)	41 (75.9)	0.198^b)
Mucinous	8 (6.9)	0	2 (8.0)	6 (11.1)
Endometrioid	5 (4.3)	0	2 (8.0)	3 (5.6)
Clear cells	5 (4.3)	0	1 (4.0)	4 (7.4)
Others	3 (2.6)	2 (5.4)	1 (4.0)	0
NI	4 (3.4)	3 (8.1)	1 (4.0)	0
Grade
02-1	45 (38.8)	9 (24.3)	10 (40.0)	26 (48.1)	0.260^b)
3	60 (51.7)	23 (62.2)	14 (56.0)	23 (42.6)
NI	11 (9.5)	5 (13.5)	1 (4.0)	5 (9.3)
Optimal surgery
Yes	82 (70.7)	22 (59.5)	20 (80.0)	40 (74.1)	0.130^b)
No	22 (19.0)	11 (29.7)	0	11 (20.4)
NI	12 (10.3)	4 (10.8)	5 (20.0)	3 (5.6)
Personal history of breast cancer
Yes	26 (22.4)	18 (48.6)	4 (16.0)	4 (7.4)	< 0.001^b)
No	89 (76.7)	18 (48.6)	21(84.0)	50 (92.6)
NI	1 (0.9)	1 (2.7)	0	0
Family history of breast/ovarian cancer
Yes	47 (40.5)	18 (48.6)	9 (36.0)	20 (37.0)	0.299^b)
No	65 (56.0)	16 (43.2)	15 (60.0)	34 (63.0)
NI	4 (3.4)	3 (8.1)	1 (4.0)	0

Values are presented as number (%). VUS, variant of uncertain significance; SD, standard deviation; FIGO, International Federation of Gynecology and Obstetrics; NI, not indicated.

^a) ANOVA test,

^b) Chi-square Pearson’s test.

Table 2.

Overall response rates after first chemotherapy in BRCA1/2-positive and sporadic epithelial ovarian cancer patients

	No. of patients	Complete and partial response	p-value
BRCA1/2 mutation	37	31 (83.8)	0.898^a)
BRCA1/2 VUS	25	22 (88.0)
BRCA1/2 wild type	54	46 (85.2)

Values are presented as number (%). VUS, variant of uncertain significance.

^a) Chi-square Pearson’s test.

Table 3.

Frequently observed BRCA1/2 mutations

Gene	Site	Mutation	Mutation type	No. (%) (n=37)	BIC data
BRCA1	Exon 11	c.3627_3628insA (p.Leu1209_Glu1210?fs)	Frameshift	5 (13.5)	Yes
BRCA1	Exon 7	c.390C>A (p.Tyr130Ter)	Nonsense	3 (8.1)	Yes
BRCA1	Exon 10	c.1399A>T (p.Lys467Ter)	Nonsense	2 (5.4)	Yes
BRCA1	Exon 11	c.4041_4042delAG (p.Arg1347_Gly1348ArgAsnfs)	Frameshift	2 (5.4)	Yes
BRCA1	Exon 11	c.3442delG (p.Glu1148Argfs)	Frameshift	2 (5.4)	Yes
BRCA2	Exon 15	c.7480C>T(p.Arg2494Ter)	Nonsense	2 (5.4)	Yes

BIC, Breast Cancer Information Core.

Table 4.

Frequently observed BRCA1/2 VUSs

Gene	Site	Mutation	Mutation type	No. (%) (n=25)	BIC data
BRCA2	Exon 18	c.8187G>T (p.Lys2729Asn)	Missense	4 (16.0)	Yes
BRCA1	Exon 16	c.5339T>C(p.Leu1780Pro)	Missense	3 (12.0)	Yes
BRCA1	Exon 16	c.4883T>C (p.Met1628Thr)	Missense	2 (8.0)	Yes

VUS, variant of uncertain significance; BIC, Breast Cancer Information Core.

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Comparison of Clinical Outcomes of BRCA1/2 Pathologic Mutation, Variants of Unknown Significance, or Wild Type Epithelial Ovarian Cancer Patients

Cancer Res Treat. 2017;49(2):408-415. Published online July 27, 2016

DOI: https://doi.org/10.4143/crt.2016.135

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Abstract
Introduction
Materials and Methods
Results
Discussion
Conclusion
NOTES
REFERENCES

Comparison of Clinical Outcomes of BRCA1/2 Pathologic Mutation, Variants of Unknown Significance, or Wild Type Epithelial Ovarian Cancer Patients

Fig. 1. Patient selection diagram. VUS, variant of uncertain significance.

Fig. 2. Progression-free survival (PFS) curves according to BRCA1/2 mutation status. Median PFS was 17 months for BRCA1/2 mutation patients, 14 months for BRCA1/2 variant of uncertain significance (VUS) patients, and 13 months for BRCA1/2 wild type patients. A log-rank test revealed longer PFS for BRCA1/2mutation than wild type patients; however, this was not statistically significant (p=0.071). No differences were detected between BRCA1/2 wild type and VUS (p=0.455) or BRCA1/2 mutation and VUS (p=0.772).

Fig. 3. Overall survival (OS) curves according to BRCA1/2 mutation status. Median OS was 33 months for BRCA1/2 mutation patients, 24 months for BRCA1/2 variant of uncertain significance (VUS) patients, and 17 months for BRCA1/2 wild type patients. A log-rank test revealed significantly longer OS for BRCA1/2mutation than wild type patients (p=0.005). No differences were detected between BRCA1/2 wild type and VUS (p=0.211) or BRCA1/2 mutation and VUS (p=0.459).

Fig. 1.

Fig. 2.

Fig. 3.

Comparison of Clinical Outcomes of BRCA1/2 Pathologic Mutation, Variants of Unknown Significance, or Wild Type Epithelial Ovarian Cancer Patients

Characteristic	Overall population (n=116)	BRCA1/2 mutation (n=37)	BRCA1/2 VUS (n=25)	BRCA1/2 wild type (n=54)	p-value
Age
Mean±SD	52.2±11.4	52.4±9.3	52.0±11.6	52.2±12.8	0.99^a)
Stage FIGO
I-II	21 (18.1)	2 (5.4)	6 (24.0)	13 (24.1)	0.059^b)
III-IV	94 (81.0)	34 (91.9)	19 (76.0)	41 (75.9)
NI	1 (0.9)	1 (2.7)	0	0
Histology
Serous	91 (78.4)	32 (86.5)	18 (72.0)	41 (75.9)	0.198^b)
Mucinous	8 (6.9)	0	2 (8.0)	6 (11.1)
Endometrioid	5 (4.3)	0	2 (8.0)	3 (5.6)
Clear cells	5 (4.3)	0	1 (4.0)	4 (7.4)
Others	3 (2.6)	2 (5.4)	1 (4.0)	0
NI	4 (3.4)	3 (8.1)	1 (4.0)	0
Grade
02-1	45 (38.8)	9 (24.3)	10 (40.0)	26 (48.1)	0.260^b)
3	60 (51.7)	23 (62.2)	14 (56.0)	23 (42.6)
NI	11 (9.5)	5 (13.5)	1 (4.0)	5 (9.3)
Optimal surgery
Yes	82 (70.7)	22 (59.5)	20 (80.0)	40 (74.1)	0.130^b)
No	22 (19.0)	11 (29.7)	0	11 (20.4)
NI	12 (10.3)	4 (10.8)	5 (20.0)	3 (5.6)
Personal history of breast cancer
Yes	26 (22.4)	18 (48.6)	4 (16.0)	4 (7.4)	< 0.001^b)
No	89 (76.7)	18 (48.6)	21(84.0)	50 (92.6)
NI	1 (0.9)	1 (2.7)	0	0
Family history of breast/ovarian cancer
Yes	47 (40.5)	18 (48.6)	9 (36.0)	20 (37.0)	0.299^b)
No	65 (56.0)	16 (43.2)	15 (60.0)	34 (63.0)
NI	4 (3.4)	3 (8.1)	1 (4.0)	0

	No. of patients	Complete and partial response	p-value
BRCA1/2 mutation	37	31 (83.8)	0.898^a)
BRCA1/2 VUS	25	22 (88.0)
BRCA1/2 wild type	54	46 (85.2)

Gene	Site	Mutation	Mutation type	No. (%) (n=37)	BIC data
BRCA1	Exon 11	c.3627_3628insA (p.Leu1209_Glu1210?fs)	Frameshift	5 (13.5)	Yes
BRCA1	Exon 7	c.390C>A (p.Tyr130Ter)	Nonsense	3 (8.1)	Yes
BRCA1	Exon 10	c.1399A>T (p.Lys467Ter)	Nonsense	2 (5.4)	Yes
BRCA1	Exon 11	c.4041_4042delAG (p.Arg1347_Gly1348ArgAsnfs)	Frameshift	2 (5.4)	Yes
BRCA1	Exon 11	c.3442delG (p.Glu1148Argfs)	Frameshift	2 (5.4)	Yes
BRCA2	Exon 15	c.7480C>T(p.Arg2494Ter)	Nonsense	2 (5.4)	Yes

Gene	Site	Mutation	Mutation type	No. (%) (n=25)	BIC data
BRCA2	Exon 18	c.8187G>T (p.Lys2729Asn)	Missense	4 (16.0)	Yes
BRCA1	Exon 16	c.5339T>C(p.Leu1780Pro)	Missense	3 (12.0)	Yes
BRCA1	Exon 16	c.4883T>C (p.Met1628Thr)	Missense	2 (8.0)	Yes

Table 1. Patient characteristics

Values are presented as number (%). VUS, variant of uncertain significance; SD, standard deviation; FIGO, International Federation of Gynecology and Obstetrics; NI, not indicated.

ANOVA test,

Chi-square Pearson’s test.

Table 2. Overall response rates after first chemotherapy in BRCA1/2-positive and sporadic epithelial ovarian cancer patients

Values are presented as number (%). VUS, variant of uncertain significance.

Chi-square Pearson’s test.

Table 3. Frequently observed BRCA1/2 mutations

BIC, Breast Cancer Information Core.

Table 4. Frequently observed BRCA1/2 VUSs

VUS, variant of uncertain significance; BIC, Breast Cancer Information Core.