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J Korean Cancer Assoc > Volume 28(2); 1996 > Article
Journal of the Korean Cancer Association 1996;28(2): 286-295.
신장세포암 세포주에 대한 세포유전학적 분석과 3 번 염색체 페인팅 Probe 적용
노경빈, 전용혁, 박선화
Application of Chromosome 3 Painting Probe and Cytogenetic Analysis on Renal Cell Carcinoma Cell Lines
Kyung Bin Rho, Yong Huch Chun, Sun Hwa Park
The analysis of recurring chromosome aberrations has become an integral part of the diagnostic and prognostic workup of many human cancers, and their molecular analyses have facilitated the identification of genes related to the pathogenesis of cancer. But the technical limitation of conventional cytogenetic method makes unable us to characterize all recognizable chromosome rearrangements. Chromosome painting is a technique of fluorescent in situ hybridization(FISH) using a chromsome-specific DNA libraries as a probe pool, and depicts an entire chromosome or a specific chromosome region. Therefore, it allows a rapid and efficient method to visualize chromosomal translocations, and thus, utilizes as an important method to assess rearrangement of marker chromosomes. In the present study, human renal cell carcinoma cell lines, such as Caki-l, A-498, CURC-II, have been analyzed to evaluate abnormalities associated with chromosome 3 using both traditional G-banding technique and chromosome painting. We investigated the cytogenetic characteristics for Caki-l, A-498, CURC-II, and found the several marker chromosomes. Using the chromosome 3 painting probe, we could successfully detect the abnormalities derived from chromosome 3, and confirmed the breakpoint of the marker chromosomes. Especially, there were many breaks in the chromosome regions 3pl2-p23 and 3ql3- q21. This method allows us to identify the origin of cytagenetically unidentifiable marker chromosomes and will be also useful to screen the cancer cells that have not-yet identified marker chromosome and the low mitotic index.
Key words: Chromosome 3 painting probe, Caki-l, A-498, CURC-II, Marker chromosomes
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