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J Korean Cancer Assoc > Volume 26(3); 1994 > Article
Journal of the Korean Cancer Association 1994;26(3): 466-474.
후두 편평상피의 전암성 및 악성병변에서 화상분석기를 이용한 핵산 검사와 Ki - 67 항체 양성세포의 분석
주형로, 이선희, 최종욱, 김인선
DNA Content and Ki - 67 Antibody Expression by Means of Image Analyzer for Laryngeal Lesions
Hyung Ro Chu, Sun Hee Lee, Jong Ouck Choi, In Sun Kim
The laryngeal epithelial cell kinetics of 26 laryngeal lesions(invasive squamous cell carcinoma 14, epithelial hyperplasia 5, laryngeal nodule 7) were studied by immunohistochemical analysis with the monoclonal antibody Ki-67, which reacts with a nuclear antigen in proliferating cells using paraffin embedded tissue. For DNA analysis, touch imprint with fresh biopsy specimens were stained with Feulgen and analyzed by image analyzer in 22 cases. 1) The positive nuclear area of Ki-67 were 32.65+-11.59% in invasive squamous cell carcino- ma, 20.14 +- 3.38% in epithelial hyperplasia and 11.66+ 3.02% in laryngeal nodule. 2) DNA aneuploidy was found in 7 cases of 10(70%) invasive squamous cell carcinomas, 2 cases of 5(40%) epithelial hyperplasia and none of laryngeal nodules. 3) Average proliferative index(S phasa+ G2/M phase) was 24.32+- 1l.33% in spuamous cell carcinama, 13.09 +- 10.90% in epithelia1 hyperplasia and 4.50 +- 1.19% in laryngeal nodule. As the results, the measurement of the DNA content and Ki-67 positive nuclear area on the small biopsy obtained by microscopic surgery used as effective factors predicting prognosis.
Key words: Epithelial hyperplasia, Ki-67, DNA content, Touch imprint
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