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J Korean Cancer Assoc > Volume 21(2); 1989 > Article
Journal of the Korean Cancer Association 1989;21(2): 413-424.
대장직장암 세포주의 성장에 영향을 주는 인자에 관한 연구
홍인규, 이성규, 박재갑, 김진복
Study on the Various Factors for Growth Stimulationof Colorectal Cancer cell Lines Using MTT Assay
In Gyu Hong, Sung Gyu Lee, Jae Gahb Park, Jin Pok Kim
The establishment of cell lines plays a important role for the study on cellular and molecular biology of cancer. lt is well known that serum free defined media is useful for the establishment of cell lines. The effect of twelve factors used as a constituents of serum free defined media, ACL-4, on seven colon cancer cell lines were tested using MTT [3-(4-5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay. Our results suggest that among twelve factors tested, transferrin and insulin and bovine serum albumin were very important factors for the growth of colon cancer cell lines. The concentration of transferrin (10 ug/ml) and insulin (20 ug/ml) in ACL-4 media was optimal to culture of the twa colan cancer cell lines, but one line, NCI-H716, graws better in higher concentration of insulin (100 ug/ml).
Key words: Colon cancer cel1 lines, ACL-4 media, MTT, Growth factors
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