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J Korean Cancer Assoc > Volume 21(2); 1989 > Article
Journal of the Korean Cancer Association 1989;21(2): 233-241.
T 세포림프계 종양에서의 T 세포수용체 유전자 재배열 현상
박성회, 송형근, 김철우, 지제근, 이상국, 김규원, 박명희, 방영주, 김노경
T - cell Receptor Gene Rearrangement in Human T - cell Lymphoid Malignancies
Seong Hoe Park, Hyung Geun Song, Chul Woo Kim, Je Geun Chi, Sang Kook Lee, Kyu Won Kim, Myoung Hee Park, Yung Jue Bang, Noe Kyeong Kim
ABSTRACT
We describe here the use of the southern blot hybridization technique to diagnose T-cell lymphoid malignancies by detecting clonal T-cell receptor gene rearrangements. DNA was isolated from human T-cell leukemia cell lines, peripheral blood or bone marrow cells of various leukemia patients and lymph nodes of malignant lymphoma patients, and analyzed for the presence of rearranged T-cell receptor genes, using radiolabeled T-cell recptor cDNA as probes. Among the specimen examined, clonal T-cell receptor gene rearrangements were found only in human T-cell leukemia cell lines and leukemic cells from T-cell acute lymphoblastic leukemia patients, not in non T-cell tumor cell ines, B cell lymphomas, and other types of leukemic cells from leukmia patients. These cells were checked with panels of monoclonal antibodies detecting T-cell, B-cell and other types of hematopoietic cells. Our studies indicate that detection of T-cell receptor gene rearrangement is a valuable methad for T-cell lineage specificity and clonal T-cell proliferation.
Key words: TCR gene rearrangement, Southern blot analysis, T-cell malingnancy
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