Journal of the Korean Cancer Association 1985;17(1): 72-85.
위선암 세포주 ( SS-1 ) 에 대한 위설암 환자의 세포살해능력에 관한 연구 - K-562 를 이용한 자연살해능력과의 비교 -
오익창, 김선회, 박재갑, 김진복
Evaluation of the Cell Mediated Cytotoxicity Against SS-1 Cell Line in the Stomach Cancer Patients - A Comparison with the NK cell activity against K-562 cell -
The human natural killer cell mediated cytotoxicity against K-562 myeloid cell line and lymphocyte mediated cytotoxicity against SS- I (Seoul National University, Adenocarcinoma of the Stomach- I) cell line were investigated in a 16-hour "Cr release assay hy using, as effectar cells, peripheral blood lymphocytes from 30 healthy donors, 60 stomach cancer patients and 15 colorectal cancer patients with a 40: 1 effector-to-target cell ratio. The lymphocyte mediated cytotoxicity of those effector cells against K-562 and SS-I was compared with each other to find any difference if present, and the value of the SS- I as a target cell in vitro cytotoxicity test was investigated. The NK cell activity of control group against K-562 was 61. 5 +- 10.5%, that of stomach cancer patients was 44.3-11. 6% and that of colorectal cancer patients was 46.4 +- 7.2%. Tbe lymphocyte mediated cytotoxicity of control group against SS- I was 66.8+9.9%, that of stomach cancer patients was 46.4+13.2% and that of colorectal cancer patients was 50.8+-11.7%. There was no difference of NK cell activity against K-562 and of cytotoxicity against SS- I between male and female sex in each target cell group. There was no evidence of carrelation between the degree of NK cell activity of atomach cancer patients and the severity of the cancer as shown with clinical atage, and the T-cell count of the stomach cancer patients. The NK cell activity did not vary with the patient's age in the stomach cancer patients. The cytotoxicity of the lymphocytes from the stomach cancer patients and the colorectal cancer patients against both target cells were significantly lower than those of control group. And there was no significant difference between the cytotoxicity of stomach cancer patients and that of colorectal cancer patients against both traget cells. The spontaneous lymphocyte mediated cytotoxicity against SS-I was significantly higher than that against K-562 in the control group; this means that SS-I has antigen or structure which can be recognized by the human NK cells. With those results, it seems that the SS-I cell line is another target cell which can be used in vitro cytotoxicity test for various cancer patients especially somach and colorectal cancer patients.
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