Abstract

The result of subcutaneous inoculation of SS-1(Seoul National University, Adenocarcinoma of the Stomach) cell line"' into nude mice is described Three million SS-I cells from passage 29 were injected subcutaneously into the subscapular area of a 57-day-old male nude mouse. But tumor was not produced. Twenty million and one hundred thousand SS-I cells from passage 35-37 were injected into a 27-day-old male nude mouse. After eight days seven million and eight hundred SS-I cells from pasaage 39-40 were injected again into the same site of the mouse. Nineteen days after initial injection: a.tumor became visible at the site of inoculation. After 53 days, the nude mouse died due to wasting by the huge tumor. Sixty-six million SS-I cells from passage 42-45 were injected into a 26-day-old male nude mouae. After 18 days a tumor became visible and it grew fast. After 53 days the tumor measured. 20.8 x 20. 1 x 15.0 mm and the tumot was subpassed to a 21-day-old male nude mouse. After 58days from the first subpassage the.' tumor measured 26. 4 x 22. 4 x 12. 5 mm and the tumor was subpassed again to a 44-day-old male nude mouse. Doubling time of the tumor was about 5.5 days. Histopathalogically the tumors produced in nude mice. were similar to the human tumor of arigin.