Purification of Agglutinin from Piellia tumberg and Its Cytotoxic Activity on Murine Leukemic Lymphoblast L5178Y

Dale Lee

Purification of Agglutinin from Piellia tumberg and Its Cytotoxic Activity on Murine Leukemic Lymphoblast L5178Y: Dale Lee; Journal of the Korean Cancer Association 1979;11(1): 31-35.

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Abstract

Pinellia tumberg agglutinin was purified from saline extract by ammonium sulfate (60%) precipitation and successive DEAE Sepharose chromatography in 0.05M Tris HCL pH 8.0 with linear NaCl gradient. An active fraction was eluted in 0.1~0.25M NaCl and its agglutinating activity to L5178Y was 2,500 units/mg with high cytotoxicity to L5178Y in saline suspension but weak toxicity in Fischer's medium.

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Purification of Agglutinin from Piellia tumberg and Its Cytotoxic Activity on Murine Leukemic Lymphoblast L5178Y

J Korean Cancer Assoc. 1979;11(1):31-35.

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