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Original Article
- Expression of Rous Sarcoma Virus Enhancer Factor Gene in Human Hepatocellular Carcinoma
- Boo Ahn Shin, Kyung Kun Kim, Chol Kyoon Cho, Hyun Chul Lee, Jong Suk Oh, Joon Haeng Rhee, Mee Young Chang
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Journal of the Korean Cancer Association 1997;29(4): 547-554.
1College of Pharmacy, Medical School, Chonnam National University, Kwangju 500-757, Korea.
2College of Dentistry, Medical School, Chonnam National University, Kwangju 500-757, Korea.
3Department of Surgery, Medical School, Chonnam National University, Kwangju 500-757, Korea.
4Department of Microbiology and Immunology, Medical School, Chonnam National University, Kwangju 500-757, Korea.
2College of Dentistry, Medical School, Chonnam National University, Kwangju 500-757, Korea.
3Department of Surgery, Medical School, Chonnam National University, Kwangju 500-757, Korea.
4Department of Microbiology and Immunology, Medical School, Chonnam National University, Kwangju 500-757, Korea.
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Abstract
PURPOSE
We have previously cloned three enhancer factor genes encoding proteins that bind to long terminal repeats (LTRs) of Rous sarcoma virus. Among these genes, RSV- EF-I gene is expressed in rat hepatoma tissues and several proliferating cell lines but not in normal rat liver tissues. We have isolated the human homologue of RSV-EF-I gene and examined its expression in human hepatocellular carcinoma tissues.
MATERIALS AND METHODS
We have screened the human genomic library and cDNA library of Hep G2 cell line derived from human hepatocellular carcinoma to isolate the human homologue of RSV-EF-I gene.
RESULTS
We have isolated one cDNA clone containing about 1.5 kb insert and sequenced. Sequence analysis reveals that this human homologue of RSV-EF-I gene has a high similarities to human YB-1 mRNA, human DNA-binding protein B (dbpB) gene and other Y-box protein genes. It is expressed in human hepatocellular carcinoma but very slightly in normal human liver tissues in Northern blot analysis.
CONCLUSION
Our data suggest that the human homologue of RSV-EF-I gene presumably belongs to Y-box protein family genes and plays a role in the transformation of the human hepatoma cells.
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