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J Korean Cancer Assoc > Volume 29(5); 1997 > Article
Journal of the Korean Cancer Association 1997;29(5): 851-866.
Significance of Epstein-Barr Virus Detection in non-Hodgkin's Lymphoma in Korea
Chang In Suh, Bum Joon Kim, Jae Won Park, Eung Soo Hwang, Yoon Hoh Kook, Chang Yong Cha
1Department of Internal Medicine, National Medical Center, Korea.
2Department of Microbiology, Seoul National University College of Medicine, Korea.
To investigate whether non-Hodgkin's lymphoma of Korea is pathogenetically associated with Epstein-Barr virus (EBV).
We analyzed fifty nine paraffin-embedded tissue and 22 fresh frozen tissue samples from non-Hodgkin's lymphoma patients for the presence of EBV sequences by polymerase chain reactions (PCR), in situ hybridization (ISH) and assessed the clonality of EBV infected cells by Southern blot hybridization. RESULT: On ISH using oligonucleotide probes corresponding to EBV-encoded small RNAs (EBERs), 17 (28.8%) of 59 paraffin-embedded tissue samples showed positive hybridization signals localized over the nuclei of the tumor cells, but PCR using primers from Internal Repeat I or EBV-determined nuclear antigen 1 gene showed positive results in only 6 (10.2%) and 5 (8.5%) samples, respectively. ISH and PCR did not detect EBV sequences in 15 paraffin-embedded tissue samples of tuberculous lymphadenitis patients. In 22 fresh frozen tissue samples, PCR detected EBV sequences in three samples from peripheral T cell lymphoma (PTCL). In two of those three samples, Southern blot analysis showed that these viral DNAs were monoclonal and of latent form.
Approximately 28.8% of non-Hodgkin's lymphoma were related to EBV in Korea. Monoclonality of those EBV DNAs implies that virus infection preceded malignant transformation, suggesting that EBV may play a role in lymphomagenesis.
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