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J Korean Cancer Assoc > Volume 30(4); 1998 > Article
Journal of the Korean Cancer Association 1998;30(4): 625-631.
Isolation of Differentially-Displayed cDNAs from a Doxrubicin Resistant Gastric Carcinoma Cell Line
Myung Soo Kang, Jae Ho Lee, Jae Gahb Park
Cancer Research Center and Cancer Research Institute Seoul National University College of Medicine.
ABSTRACT
PURPOSE:
This study was aimed to isolate cDNAs putatively associated with doxorubicin resistance or sensitivity in gastric carcinoma cell line.
MATERIALS AND METHODS:
A doxorubicin-sensitive parental SNU-16 and doxorubicin resistant SNU-16DOX cell line were used. Differential display-PCR(DD-PCR) was employed to screen for differentially expressed cDNA fragment either in parental or resistant cell line and followed by subtractive hybridization to discriminate true positive from false positive clones. The sequences were determined and compared to the sequence data base registered at the GenBank.
RESULTS:
Four clones(16, 19, 21, and 22 clone) were isolated, of which three(16, 19 and 21 clone) was downexpressed, and one(22 clone) was overexpressed in doxorubicin resistant cell line. All four clones were found to be novel sequences. Further analysis for these clones are under characterization.
CONCLUSION:
Four partial cDNA clones that are putatively associated with doxorubicin resistance or sensitivity in gastric carcinoma cell line were isolated.
Key words: Doxorubicin resistance;cDNA;Differential display-PCR;Subtractive hybridization
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