Cancer Research and Treatment

Original Articles

Synergistic Effect of Cisplatin and 5 - Fluorouracil Against Gastric and Colon Cancer Cell Line: Yoon Sik Kang, Dong Young Noh, Jae Gahb Park, Kuk Jin Choe; J Korean Cancer Assoc. 1990;22(3):490-505.

Abstract PDF: The purpose of this study was to elucidate the synergistic effect of cisplatin and 5-fluorouracil against human gastric and colon cancer cell lines. Since it is virtually impossible to document supraadditive tumor cell kill in vivo, this in vitro experiment implicates the interaction of these agents at a cellular level. We used an in vitro tetrazolium-based colorimetric assay for cytotoxicity (MTT assay) and an isobologram analysis to test combination effects of the two drugs against gastric and colon cancer cell lines, SNU-1, SNU-5, SNU-16, SNU-CI, SNU-C2A, SNU-C4, SNU-C5 those were established at cancer research institue of Seoul National University and NC1-N87 from NCI, VSA. As a resuh, those drug combination applied to this experiment didnt show a synergistic effect except in SNU-1 cell line demonstrated partly synergistic effect. Then we conclude that there are lack of in vitro synergistic effect between cisplatin and 5- fluorouracil against gastric and colon cancer cell lines, and that methodology we adopted can be one index ta check the combinatian effect of anticancer drugs.

In Vitro Chemosensitivity Test for the Evaluation of Efficiency of Hyperthermia in Gastrointestinal Cancer Cell Lines: Jeong Hwan Yook, Byeong Yul Ahn, Geum Hee Koo, Hun Seo, Choon Sik Jeong, Sung Tae Oh, Byung Sik Kim, Kun Chun Park, Jin Cheon Kim; J Korean Cancer Assoc. 1999;31(5):931-938.

Abstract PDF: PURPOSE
This study was designed to establish the experimental background of intra- peritoneal hyperthermo-chemotherapy in gastrointestinal cancer.
MATERIALS AND METHODS
We established stomach cancer cell lines; KATO-III, MKN45, AMC1 and colon cancer cell lines; AMC5, AMC6, CloneA, CCL188, C106, KM-12C. We performed chemosensitivity test by using MTT assay and calculated ICso of each chemotherapeutic agent. We confirmed antitumor effect of hyperthermia at 40C and 43C and antitumor synergistic effect with each chemotherapeutic agent at 40C and 43C.
RESULTS
The ICso was calculated in 7 (78%) of 9 cell lines for 5-FU, 6 (67%) for MMC, 5 (56%) for ADM, 1 (11%) for CDDP and VP-16. Antitumor effect of hyperthermia at 40C was not found, but, that at 43C was found except KATO-III and AMC6. In stomach cancer cell lines, antitumor synergistic effect of hyperthermia with anticancer drugs at 43C was found in VP-16 for MKN45 and KATO-III and in all of 5 drugs for AMC1. In colon cancer cell lines, this effect at 43C was found in all of 5 drugs for CCL188, in S-FU, CDDP, ADM for AMC5, in 5-FU, MMC, ADM, VP-16 for CloneA, KM-12C, and in 5-FU, CDDP, MMC, ADM for C106.
CONCLUSION
Hyperthermia itself had antitumor effect at 43C. Hyperthermo-chemotherapy had antitumor synergistic effect, especially at 43C.

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