Cancer Research and Treatment

Original Articles

Expressions of Phospholipase D, MMP-2, and TIMP-2 in Adenocarcinomas of the Lung: J H Park, K M Lee, J K Shin, S K Park, H K Kim, S S Kim, S D Lim, J R Huh, J Y Roh, P G Suh, S H Ryu; J Korean Cancer Assoc. 2000;32(2):398-406.

Abstract PDF: PURPOSE
Metastatic spread depends critically upon the invasiveness of tumor cells, i.e. their ability to breach basement membranes by elaborating and secreting specific proteolytic enzymes such as gelatinase A (MMP-2). Phospholipase D (PLD) is believed to play an important role in cell proliferation and tumorigenesis. Also several studies suggested that activation of PLD and consequent generation of phosphatidic acid are involved in signal propagation pathway leading to induction of MMP-2. However, the relation between PLD and MMP-2 is not fully studied in lung cancer tissue, yet.
MATERIALS AND METHODS
Adenocarcinomas of the lung from 20 patients were studied for immunohistochemical expressions of PLD, MMP-2 and TIMP-2 to assess their diagnostic and prognostic importance in lung tumors.
RESULTS
With decreasing tumor differentiation, there was a progressive increase of MMP-2 and PLD and TIMP-2 expression decreased. Type IV collagenase expression was significantly associated with the presence of lymph node metastases, with moderate to strong expression, presented in 60% node positive tumors compared with none of the node negative tumors (p < 0.05). PLD was increased in tumor (strong: 60%) with nodal metastases compared with those without nodal metastases (p < 0.05), Increase of MMP-2 and PLD expression was associated with loss of TIMP-2 expression.
CONCLUSION
In correlation expression of the immunohistochemical markers and invasion, PLD as well as type IV collagenase and TIMP2 expressions was found to be a predictor of invasiveness. Measurable alterations in MMP-2 and TIMP-2 and in particular, expression of PLD may be an important factor to assess invasiveness in resectable adenocarcinoma of the lung.

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Alteration of Oleate-Phospholipase D Activities in Some Cell Lines after Irradiation: Chul Yong Kim, Myung Un Choi, Myung Sun Choi; J Korean Cancer Assoc. 1997;29(6):944-953.

Abstract PDF: PURPOSE
Phospholipase D (PLD) catalyzes the hydrolytic cleavage of terminal phosphate diester bond of glycerophopholipids to produce phosphatidic acid (PA). PLD plays an important role in signal transduction and is known to be involved closely in cancer promotion, inflammation, and other cell responses. In order to evaluate radiation effect in tumor cells, various cells were screened for PLD activities and examined their radiation effects on PLD following gamma- ray irradiation.
MATERIALS AND METHODS
PLD activities in 19 species of cell were measured by radioactive isotope method with 1,2 - di [1-14C] phosphatidylcholine in the presence of oleate. Among the cell lines examined, VERO 76, L 1210 and P 388 were selected and examined for their effects of metal ions and agonists on PLD activities before and after irradiation by Co-60 teletheraphy unit.
RESULTS
The activities of oleate-PLD were observed in 11 species among 19 cell lines examined. VERO 76 and L 1210 cells showed that the PLD activity increased immediately after irradiation and reached to 150~200% of the control levels. The activation of PLD in response to gamma-ray was maximum at 20 Gy. In irradiated VERO 76, the stimulatory effect of Mg2+ was reduced and the activation of PLD by agonists in irradiated cells vary from those of the control cells.
CONCLUSION
The activation effect of irradiation on PLD activity observed strongly implies that the PLD activity is closely related to the phenomena of cell necrosis. Therefore the cell lines examined here could provide a good source for the study of radiobiology that cover from cell death to cancer promotion.

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