Cancer Research and Treatment

Predictive Value of the nProfiler 1 Assay for the Efficacy of Adjuvant S-1–Based Doublet Chemotherapy in Stage III Gastric Cancer: A Post-Hoc Analysis of a Randomized Phase III Trial: Dong Ki Lee, Choong-kun Lee, Hyo Song Kim, Sun Jin Sym, Dae Young Zang, Ki Hyang Kim, Joo Han Lim, Hae Su Kim, Kyung Hee Lee, Heon Yung Gee, Sun Young Rha, Hyunki Kim, Minkyu Jung; Received July 25, 2024 Accepted November 9, 2024 Published online November 12, 2024; DOI: https://doi.org/10.4143/crt.2024.705 [Epub ahead of print]

Abstract PDF Supplementary Material PubReader ePub: Purpose
The nProfiler 1 Stomach Cancer Assay (nProfiler1), designed to predict responses to fluorouracil-based adjuvant chemotherapy, measures the expression of four gastric cancer target genes (GZMB, WARS, SFRP4, and CDX1). The randomized phase III POST trial aimed to compare the efficacies of two adjuvant S-1-based doublet chemotherapies: S-1 plus cisplatin (SP) and S-1 plus docetaxel (DS). This study aimed to validate the nProfiler1 assay using a distinct cohort from the POST trial.
Materials and Methods
The nProfiler1 assay stratifies patients into three groups (low-risk, intermediate-risk, and high-risk) using the prognostic single-patient classifier and two groups (chemotherapy-benefit and no-benefit) using the predictive single-patient classifier. The nProfiler1 assay was applied to formalin-fixed paraffin-embedded slides obtained from the POST trial. Disease-free survival (DFS) and overall survival (OS), including 5-year survival rates, were calculated for the enrolled patients.
Results
Of the 153 patients in the POST trial, 118 were included in the post-hoc analysis. With a median follow-up of 57.9 months, no significant difference in DFS or OS was observed between the SP and DS groups. The prognostic single-patient classifier predicted the OS in the SP group (p=0.043) but not in the DS group (p=0.594). The chemotherapy-benefit group exhibited numerically longer DFS than the no-benefit group in the SP and DS groups.
Conclusion
The nProfiler1 assay offers valuable insights into the prognosis and efficacy of adjuvant chemotherapy based on fluorouracil plus platinum doublet regimens but not docetaxel-containing regimens. Further validation with larger patient cohorts and different regimens is warranted.

1,011 View
99 Download

Expression of Maspin is associated with the Intestinal Type of Gastric Adenocarcinoma: Seong Man Kim, Seong Jin Cho, Woo Young Jang, Duck Hwan Kim, Hyung Sik Shin, Myoung Kuk Jang, Hak Yang Kim, Eun Sook Nam; Cancer Res Treat. 2005;37(4):228-232. Published online August 31, 2005; DOI: https://doi.org/10.4143/crt.2005.37.4.228

Purpose

Maspin is known as a tumor suppressor gene, but its significance has been questioned in various human cancers. The aim of this study was to investigate the expression pattern of Maspin in human gastric adenocarcinomas and its possible correlation with clinicopathological findings.

Materials and Methods

The expression of Maspin mRNA was measured by nested RT-PCR using 60 frozen adenocarcinomas of the stomach and 31 noncancerous tissues from the proximal resection margin. Immunohistochemical study for Maspin protein expression was carried out using 62 paraffin-embedded tissues, composed of both cancer and noncancerous tissues.

Results

Maspin mRNA expression was detected in 80.0% (48 of 60) of the gastric adenocarcinomas, but in only 22.6% (7 of 31) of the normal gastric mucosa (p<0.001). The positive rate of Maspin protein expression was higher in the adenocarcinomas than the normal tissues (62.9% vs. 27.4%, p<0.05). In addition, the intestinal type of tumors showed significantly higher expression levels compared to the diffuse type of tumors (81.5% vs. 48.6%, p<0.05).

Conclusion

Our results suggest that Maspin is frequently expressed in human gastric cancers, and its expression might be associated with tumorigenesis of the intestinal type of gastric cancer.

Citations

Citations to this article as recorded by

The paradoxical role of SERPINB5 in gastrointestinal cancers: oncogene or tumor suppressor?
Shuyan Zeng, Jiayu Zhang, Wanyi Jiang, Chunyan Zeng
Molecular Biology Reports.2025;[Epub] CrossRef
P176S Mutation Rewires Electrostatic Interactions That Alter Maspin Functionality
Muhammad Ayaz Anwar, Muhammad Haseeb, Sangdun Choi, Kwang Pyo Kim
ACS Omega.2023; 8(31): 28258. CrossRef
SERPINB5 is a novel serum diagnostic biomarker for gastric high-grade intraepithelial neoplasia and plays a role in regulation of macrophage phenotypes
Xiuhong Huang, Xiaoli Xie, Ning Kang, Ran Qi, Xue Zhou, Yijun Wang, Huiqing Jiang
Translational Oncology.2023; 37: 101757. CrossRef
Analysis of co-expression networks for circular RNAs and mRNAs reveals that circular RNAs hsa_circ_0047905, hsa_circ_0138960 and has-circRNA7690-15 are candidate oncogenes in gastric cancer
Zhiyong Lai, Yang Yang, Yichao Yan, Tao Li, Yansen Li, Zhu Wang, Zhanlong Shen, Yingjiang Ye, Kewei Jiang, Shan Wang
Cell Cycle.2017; 16(23): 2301. CrossRef
The roles of maspin expression in gastric cancer: a meta- and bioinformatics analysis
Hua-Chuan Zheng, Bao-Cheng Gong
Oncotarget.2017; 8(39): 66476. CrossRef
Maspin: molecular mechanisms and therapeutic implications
Thomas M. Bodenstine, Richard E. B. Seftor, Zhila Khalkhali-Ellis, Elisabeth A. Seftor, Philip A. Pemberton, Mary J. C. Hendrix
Cancer and Metastasis Reviews.2012; 31(3-4): 529. CrossRef
Prognostic value of nuclear maspin expression for adjuvant 5-fluorouracil-based chemotherapy in advanced gastric cancer
KE-FENG LEI, BING-YA LIU, XIAO-LONG JIN, YAN GUO, MIN YE, ZHENG-GANG ZHU
Experimental and Therapeutic Medicine.2012; 3(6): 993. CrossRef

10,108 View
35 Download
7 Crossref

Expression of Matrix Metalloproteinases and Its Inhibitor in Gastric Adenocarcinoma: Sung Chul Lim; Cancer Res Treat. 2001;33(3):199-206. Published online June 30, 2001; DOI: https://doi.org/10.4143/crt.2001.33.3.199

Abstract PDF

PURPOSE
Matrix metalloproteinases (MMPs) have been associated with tumor cell invasion and metastasis by mediating the degradation of extracellular matrix components. A tissue inhibitor of metalloproteinases (TIMPs) has been reported to inhibit tumor invasion by means of an inactivation of matrix metalloproteinases. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. Therefore, MMPs and their inhibitors constitute promising agents for developing anticancer therapies. In the present study, the authors investigated the correlation between the expressions of TIMP-1 and MMPs, and the clinical outcome.
MATERIALS AND METHODS
Immunohistochemical staining of MMP-2, -3 and -9, and TIMP-1 was performed on paraffin-embedded tissue sections of 38 early gastric carcinomas and 61 advanced gastric carcinomas.
RESULTS
MMP-2 and -9 were found mainly in tumors of the intestinal type and less frequently in those of diffuse type. There were positive correlations between the presence of MMP-2 and -9 and lymph node status. There were inverse correlations between the TIMP-1 expression and tumor invasiveness.
CONCLUSION
These results suggests that clinical outcomes such as the depth of invasion or metastasis are closely related to the expression of TIMP-1, MMP-2 and MMP-9.

Citations

Citations to this article as recorded by

Research on the mechanisms of taraxerol for the treatment of gastric cancer effect based on network pharmacology
Bingjie Huo, Yanru Song, Bibo Tan, Jianbo Li, Jie Zhang, Fengbin Zhang, Liang Chang
International Journal of Immunopathology and Pharmacology.2022;[Epub] CrossRef
Potential Antioxidant and Antiphotoaging Effects of Fagopyrum esculentum Honey on Human Dermal Fibroblasts
Hye-Bin Kim, Sungkwan An, Seunghee Bae
Asian Journal of Beauty and Cosmetology.2022; 20(1): 43. CrossRef
Regulatory Mechanism and Experimental Verification of Patchouli Alcohol on Gastric Cancer Cell Based on Network Pharmacology
Yanru Song, Liang Chang, Xiaoyuan Wang, Bibo Tan, Jianbo Li, Jie Zhang, Fengbin Zhang, Lianmei Zhao, Guangjie Liu, Bingjie Huo
Frontiers in Oncology.2021;[Epub] CrossRef
Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis
Lei Wu, Lifen Wang, Xiangguo Tian, Junyong Zhang, Hua Feng
BMC Complementary Medicine and Therapies.2020;[Epub] CrossRef
Paeonol exerts potential activities to inhibit the growth, migration and invasion of human gastric cancer BGC823 cells via downregulating MMP-2 and MMP-9
Zhong-Kuan Lyu, Chang-Ling Li, Yan Jin, Yu-Zhao Liu, Xi Zhang, Fang Zhang, Lu-Ning Ning, Er-Shun Liang, Min Ma, Wei Gao, Ming-Xiang Zhang, De-Shan Liu
Molecular Medicine Reports.2017; 16(5): 7513. CrossRef

3,934 View
23 Download
5 Crossref

The Inhibitory Effect of Amiloride on the Growth of Human Gastric Carcinoma Cells in Vitro: Seung Su Kang, Duck Kyung Kong, Chee Whan No, Byung Joo Choi, Moo In Park, Seun Ja Park, Keun Young Park, Ja Young Koo; J Korean Cancer Assoc. 2001;33(2):113-120.

Abstract PDF: PURPOSE
In the present study the effects of amiloride on the growth of human gastric adenocarcinoma cell line, AGS cells were examined with or without the addition of 5-fluorouracil (5-FU) in vitro.
MATERIALS AND METHODS
The growth of AGS cells was examined by counting number of cells on two and four days post-treatment with 50 micrometer, 100 micrometer, 200 micrometer, 400 micrometer, 800 micrometer, amiloride, and 0.1 microgram/ml, 0.3 microgram/ml 5-FU, after plating AGS cells into 6 well plates at a density of 10 x 10(4) cells/well. The reversibility of the effects of amiloride was examined on two to eight days post-treatment with 400 micrometer amiloride after seeding 2 x 10(4) cells/dish. Cell cycle analysis was performed after four day-treatment with 400 micrometer amiloride.
RESULTS
Amiloride (50~800 micrometer) significantly inhibited the growth of AGS in a dose-dependent fashion (p<0.05). The inhibitory effect of amiloride on growth of AGS was reversible since removal of amiloride after 24 hours treatment led to resumption of rapid growth up to control levels. Amiloride combined with 5-FU markedly inhibited the growth of AGS in a dose-dependent fashion compared to that of amiloride or 5-FU alone (p<0.05). The fraction of S phase, G0-G1 phase and G2-M phase was 19.3%, 55.7%, 18.8%, in the amioride group (400 micrometer) and 43.9%, 37.4%, 25.1% in the control group, respectively, showing significantly higher G1 fraction in amiloride group compared to control.
CONCLUSION
This is the first paper which reported that amiloride inhibited in vitro growth of human gastric adenocarcinoma cells and that its effect of growth inhibition may be synergistic with 5-FU. Amiloride given with or without 5-FU may be useful agent in the treatment of gastric carcinomas. The inhibitory effects of amiloride on the growth of AGS may be mediated in part by blocking G1-S transition of cell cycle.

3,214 View
18 Download

Evaluation of the New UICC Staging System for Gastric Carcinoma: Hyeong Myeong Back, Sung Joon Kwon, Oh Jung Kwon, Pah Jong Jung, Kwang Su Lee, Jin Young Kwak, Kyu Young Jun, Chi Kyu Won; J Korean Cancer Assoc. 1999;31(1):54-61.

Abstract PDF: PURPOSE
There are several kinds of classificatian dealing with the staging of the gastric adenocarcinoma. However, such different staging systems pose difficulty in the inter- institutional or intemational comparison of the disease status and the treatment results. The purpose of this study is to evaluate each staging system and to assess the usefulness of the new UICC-TNM staging system (1997) for gastric adenocarcinoma. MATERIAL AND METHODS: We retrospectively analysed 473 cases of gastric adenocarcinoma who were operated at the Department of General Surgery, Hanyang University Hospital during the period from 1992 to 1996. Using these cases, we analyzed their cumulative 5-year survival rate according to three kinds of staging systems; old UICC-TNM staging system (1987), new UICC-TNM staging system (1997), and the Japanese staging system for gastric carcinoma (1993).
RESULTS
The follow up rate was 94.3% and the median follow up period was 30.3 months. All of these three systems showed a statistically significant survival difference according to their different classifications. When the distribution of stage between old and new UICC-TNM staging system was compared, 95 cases (20.1%) were subjected to stage shifting, which involves 12.1% of up-staging and 8.4% of down-staging. Stage shifting was most prominent in stage IIIb (68.8%). The cumulative 5-year survival rate according to the new UICC-TNM staging system was 99.1% in stage Ia, 81.4% in stage Ib, 75.2% in stage II, 45.9% in stage IIIa, 21.0% in stage IIIb, and 19.4% in stage IV.
CONCLUSION
We conclude that the new UICC-TNM staging system is simple, practically convenient, and highly reproducible, and it showed a statistically significant survival difference according to their staging classification.

3,051 View
12 Download

Expression of EGF Receptor Family Genes and Proteins in Gastric Cacner ( EGFR , c-erbB-2 , c-erbB-3 , c-erbB-4 ): Hae Wan Lee, Eun Young Choi, Chang Dae Bae, Han Kwang Yang, Woo Ho Kim, Jin Pok Kim; J Korean Cancer Assoc. 1998;30(5):853-868.

Abstract PDF: PURPOSE
to evaluate the relationship between the expression of EGF receptor gene family and clinico-pathologic parameters.
MATERIALS AND METHODS
We compared adenocarcinoma tissue with normal mucosa obtained from same patients in 60 cases. The amplifications of DNA was examined by slot blot, while the expression of mRNA by ribonuclease protection assay, and that of protein by immunohistochemistry.
RESULTS
Expression of EGFR mRNA was observed in 9 of 59, that of c-erbB-2 mRNA in 8 of 57, that of c-erbB-3 mRNA in 4 of 60, and that of c-erbB-4 mRNA in 13 of 59. The expression of EGFR in expanding type showed a higher tendency than that in infiltrative type. The expression of c-erbB-2 in poorly differentiated adenocarcinoma showed a higher tendency than that in well differentiated adenocarcinoma. And expression of c-erbB-2 was correlated with presence of endolymphatic tumor emboli. No significant correlation was observed between expression of EGFR mRNA and that of its protein or amplification of its DNA. Similarly, No clear relationship between c-erbB-2 gene amplification and expression of mRNA or proteins was detected.
CONCLUSION
EGFR, c-erbB-2, c-erbB-3, and c-erbB-4 were expressed in gastric adenocarcinoma in Korea. The presence of EGF receptor gene family by various tumor cells suggested that it may play an important role in adenocarcinoma. Therefore further studies are currently being carried out to clarify the role of these oncogenes in tumor behavior and gastric carcinogenesis.

2,339 View
13 Download

Immunohistochemical Analysis of MHC Class 2 (HLA-DR / DP), ICAM-1, CD68(+) Macrophage Expression in Gastric Adenocarcinoma: Eon Sub Park, Seong Nam Kim, Tae Jin Lee, Im Joong Yoon, Yong Kyoo Shin, Jae Hyung Yoo; J Korean Cancer Assoc. 1998;30(1):40-54.

Abstract PDF: PURPOSE
Gastric adenocarcinoma is the most common malignant tumor in Korea and immunochemotherapy can be alternative method of the treatment for it. So we evaluated several immunologic markers, Major Histocomatibility (MHC) Antigen and Intercellular Adhesion Molecule (ICAM)-1 which play an important roles in cellular immune response of the host to the tumar cells, HLA-DR/DP antigens, one of the MHC class II which is expressed in various conditions, CD 68 antigen which are also important factor in immune response to the tumor cells.
MATERIALS AND METHODS
We compared the expression of MHC class II (HLA-DR/DP) antigens, ICAM-1 and the number of tumor-infiltrating macrophages presenting CD68 antigen in formalin-fixed paraffin-embedded tissue sections of 95 gastric adenocarcinomas using immunohistochemistry. In addition to analyze the relationship between expression of these antigens in gastric adenocarcinoma, histolopathologic findings such as tumor invasion, regional lymph node metastasis and histologic differentiation are evaluated.
RESULTS
The rate of HLA-DR/DP expression was 60% and strongly associated with tumor differentiation, invasion and regional lymph node metastasis. ICAM-1 was expressed in 15% and slightly increased in well-differentiated carcinoma. The lack of expression of ICAM-1 was observed in high invasive tumor (T 4). CD 68(+) macrophages counts were significantly increased in around the tumor cells, compared to normal epithelia. HLA-DR/DP expression and infiltrating CD 68(+) macrophage numbers were significantly associated (p<0.05), but there was no correlationship between ICAM-1 and CD 68(+) macrophage numbers.
CONCLUSION
It was considered that enhanced expression of HLA-DR/DP antigens, ICAM-1 and CD68(+) macrophages in gastric adenocarcinomas may be an immunophenotypic deviation. HLA-DR/DP and CD68(+) macrophages infiltration showed correlationship with tumor invasion and regional lymph node metastasis, that they may be used as a prognostic factor of the tumor growth.

2,938 View
30 Download

Significant Correlation of Hepatocyte Growth Factor Level with Progression of Gastric Adenocarcinoma: Sang Uk Han, Jae Ho Lee, Wook Hwan Kim, Hee Jung Wang, Yong Kwan Cho, Myung Wook Kim; J Korean Cancer Assoc. 1997;29(3):367-374.

Abstract PDF: PURPOSE
Hepatocyte growth factor (HGF) is a modulator of epithelial cell proliferation and motility. In this study, we measured the level of HGF in sera and tumor extracts of gastric adenocarcinoma using an enzyme-linked immunoassay and evaluated its association with tumor progression.
MATERIALS AND METHODS
The level of HGF in the sera of seventy-five patients with gastric adenocarcinoma and in the tumor extracts of forty-two tumors were examined in this study. The level of HGF was determined by an Immunis HGF EIA kit (Institute of Immunology).
RESULTS
The mean level of HGF in the sera of patients was 0.26+/-0.19 ng/ml, which was significantly higher than in those of healthy controls (0.14+/-0.07 ng/ml, p<0.05); the levels of HGF in the sera of patients increased according to the progression of the stage of cancer (p<0.05). The mean level of HGF in tumor extracts was 8.22+/-9.27 microgram/g protein, which was significantly higher than in those of healthy controls (1.95+/-1.45 microgram/g protein, p<0.05); the levels of HGF in the tumor extracts were correlated significantly with the progression of the tumor stage (p<0.05). The mean level of HGF in the tumors of diffuse type was 11.28+/-11.74 microgram/g protein, which was significantly higher than in those of intestinal type (5.16+/-4.31 microgram/g protein, p<0.05).
CONCLUSION
HGF may play an important role in the progression and differentiation of gastric adenocarcinoma.

2,949 View
16 Download

Immunohistochemical Analysis of the Expression of Cathepsin D in Human Gastric Adenocarninoma: Sang Uk Han, Hee Jae Joo, Yong Kwan Cho, Kyung Po Lee, Myung Wook Kim; J Korean Cancer Assoc. 1997;29(1):93-102.

Abstract PDF: PURPOSE
Cathepsin D is an acidic lysosomal proteinase involved in intracellular protein turnover. Increased levels of this enzyme have been reported to be indicators of aggressive tumor behavior in some human tumors. In gastric cancer, increased expression of cathepsin D has been reported to be an independent prognostic factor.
MATERIALS AND METHODS
We used standard immunohistochemical techniques on formalin- fixed, paraffin-embedded tissues to examine the expression of the cathepsin D in fifty five gastric adenocarcinomas. And we compared these with other indicators of aggressive tumor behavior including stage of disease, tumor size, lymphatic invasion, neural invasion, Lauren classification, disease recurrence and survival.
RESULTS
Positive granular cytoplasmic staining for cathepsin D was detected in 100% of the tumors and strongly positive staining was found in 53%. However, the intensity of the staining varied from cell to cell in the same carcinoma tissue as well as among samples. Positive staining also was seen in normal foveolar epithelial cells, parietal cells, macrophages and ganglion cells. Our results did not show any correlation between the expression of cathepsin D and other indicators of aggressive tumor behavior. But the group having more intensely stained margins showed the tendency to frequent lymphatic invasion.
CONCLUSION
We conclude that the results obtained using polyclonal antibodies to cathepsin D do not support the prognostic usefulness of immunohistochemical analysis of this proteinase in tumor cells in human gastric adenocarcinoma, but this study may offer some useful indicator for further pathophysiological studies on gastric adenocarcinoma.

2,804 View
17 Download

Expression of Cellular Oncogenes in Korean Gastric Adenocarcinomas: Hae Keun Yoon, Jin Pok Kim, Jeong Sun Seo; J Korean Cancer Assoc. 1989;21(2):269-290.

Abstract PDF: All of oncogenes was first described as part of the genome of RNA tumor viruses and were subsequently shown to be of cellular origin. These genes termed cellular oncogenes (c-onc)-appear to have been the evolutionary progenitors of viral oncogenes. It is strongly suggested that cellular oncogenes may possess an oncogenic potential. It is important to elucidate which and how cellular oncogenes can be activated for specific tissue types of human tumors. Transforming genes in human bladder cell lines have been identified as the c-onc genes known as c-Ha-ras and c-Ki-ras of point mutation type. Recently it was found that, 10% (detection rate) of fresh human bladder tumors are related to point mutation. Therefore considering the multistep carcinogenesis in human tumor in vivo, it seems to be very important to assess the expression level of multiple c-onc genes in freshly obtained human tumor tissues. We investigated the level of gene expression of 5 c-oncogenes (c-Hr-ras, c-Ki-ras, c-myc, c-myb and r.-fps) in 17 cases of gastric adenocarcinomas (stage II: 4 cases, stage Ill: 13 cases). DNA probes using for DNA-RNA hybridization technique were all cellular oncogenes except ras-Ki-gene (v-ras Ki). The results are summarized as follows: 1) Some of gastric adenocarcinomas showed the increased expression of cfps and c-myc (10 of 17 cases, and 9 of 17 cases, respectively). Signet ring cell type of adenocarcinoms showed the high expression of c-Ha-ras. 2) The other two oncogenes, c-Ki-ras and c-myb were expressed very infrequently in the gastric adenocarcinomas. 3) The coincidence of expression of two oncogenes, such as c-myc and cfps was found in 9 of 10 cases. 4) The regional lymph node metastasis rate was below 5g in gastric adenocarcinoma cases with high expression of c-fps and c-myc (5 out of 6). 5) There is a slight decreasing tendency in cfps and c-myc expression rates from Borrmann type I to type IV. 6) There is no significant difference in c-myc and c-fps expression levels and rates between intestinal type and diffuse type of gastric adenocarcinoma classfied by Lauren's method.

2,627 View
13 Download

A Case of gastric Adenocarcinoma Associated with Chronic Myelogenous Leukemia: Joong Ha Hwang, Hyun Hae Bae, Kyung Ho Kim, Joo Hyung Lee, Hye Jeong Yoon, Ho Yeong Lee, Choong Ki Lee; J Korean Cancer Assoc. 1994;26(6):991-997.

Abstract PDF: The incidence of multiple primary cancer is increasing recently due to various reasons. A case of gastric cancer was developed during the course of treatment of CML and the interval between CML and subsequently found gastric adenocarcinoma was l6 months. We report a case of gastric adenocarcinoma with CML with review of literature.

3,047 View
15 Download

End Results of Surgical Treatment of 354 Patients with Gastric Adenocarcinoma: Kyung Suk Chung, Sung Ha Moon, Hyun Wook Shin, Young Cheol Lee, Joo Seop Kim, Bong Wha Chung, Jae Jung Lee, Chul Jae Park, Young Cheol Jeon, Myung Seok Lee, Woo Joong Kim; J Korean Cancer Assoc. 1995;27(1):28-35.

Abstract PDF: The surgical results of 354 patients with gastric adenocarcinoma treated at the Department of Surgery, Kangnam Sarced Heart Hospital, Hallym University from l984 to 1993 were report- ed. The survival rate of the 354 patients was compared with reference to categories of prognostic factors(univariate analysis) and significant factors affecting survival were identified by multivariate analysis using the Cox's proportional hazard model. The resection rate was 93.5% and the operative mortality within 1 month was 2.5%. The 5- year survival rates were 49.2% for the entire group of patients, 52.0% for patients who under-went resection, 72.3% for patients who underwent curative resection, and 18.4% for patients who underwent palliative resection. The 5-year survival rates according to the TNM stage were 85.4% for stage I, 56.0% for stage II, 38.2% for stage III, and 10.3% for stage IV. Univariate analyses showed a significant difference in survival in relation to age, primary tumor factor(T), reaional lymph node factor(N), distant metastasis factor(M), macroscopic type, size of tumor, type of operation, TNM stage and curability of surgery. Multivariate analysis using the Coxs proportional hazard model in a stepwise fashion identified a final set of three significant variab1es: curability of surgery(P=0.0002), TNM stage(P=0.0007), and macroscopic type(P=0.0008). These results suggest that continuing efforts to increase rate of less advanced cancers and curative resection with systematic lymph node dissection in advanced cancers are necessary for an improvement of the end results of surgical treatment.

3,102 View
12 Download

Idiopathic Thromborcytopenic Purpura - like Syndroma in a Patient with Gastric Adenocarcinoma: Seong Hin Hong, Joon Ho Song, Seok Jung, Seon Hoo Kim, Jae Who Park; J Korean Cancer Assoc. 1995;27(1):138-144.

Abstract PDF: Idiopathic thrombocytopenic purpura(ITP)-like syndrome is a very rare paraneoplastic syndrome which is caused by autoimmune mediated platelet destruction. Thrombocytopenia in malignant tumor is usually known to be caused by several mechanisms including marrow replacement with tumor, marrow hypoplasia secondary to chemotherapy, platelet consumption due to activation of the coagulation cascade and the mechanism of autoimmune mediated platelet destruction has been also described. While most cases are documented in lymphopro- liferative disorders, it is extremely rare in solid tumor. We experienced a case of thrombocytopenia which could not be explained by usual mechanisms in a patient with gastric adenocarcinoma. In the case, ITP-like syndrome was suspected because no evidence of DIC was faund and all trials of platelet replacement were failed because of refractory response. As expected, the patient showed positive response to anti-platelet antibody, and spontaneous remission was achieved by administration of high dose immunoglobulin and prednisone with chemotherapy simultaneausly. We did not splenectomy, because platelet number was maintained well with above therapy. We report a case of ITP-like syndrome in gastric adenocarcinoma patient and so far as can be determined there has not been reported.

2,340 View
13 Download

A Case of Multiple Cutaneous Metastasis from Gastric Adenocarcinoma: Hun Sik Jeong, Kwang Ho Kim, Yeon Suk Kim, In Suk Song, Dong Gu Choi, Dong Bok Shin; J Korean Cancer Assoc. 1995;27(5):893-897.

Abstract PDF: Cutaneous multiple metastases from internal carcinoma are relatively rare, especially from gastric cercinoma. We report a case of multiple cutaneous metastasis from gastric adenocarcinoma in 51 year old male patient who have multiple variable sized erythromatous nodules and macule on the trunk, left axilla, and right thigh.

2,624 View
15 Download

An Immunohistochemical Study of Correlation between Expression of Plasminogen Activator Inhibitor-1 and Lymph Node Metastasis in Gastric Adenocarcinoma: Yeong Ok Kim, Man Ha Huh; J Korean Cancer Assoc. 1996;28(3):405-141.

Abstract PDF: Plasminogen activator inhibitor-1 (PAI-1) is inhibitor of plasminogen activator(PA) which is serine protease involving proteolytic degradation of the extracellular matrix and tumor invasion and metastasis. Detailed analysis of the PAI-1 in malignant tissues has not yet been reported. To investigate whether expression of PAI-1 in gaatric adenacarcinoma correlates with lymph node metastasis, immunohistochemical analysis using monoclonal antibody for this antigen was performed in 101 cases of gastric adenocarcinomas. Specific immunostaining was detected in the cytoplasm of the cancer cells and was not detected in stromal cells. Expression of PAI-I was evident in 61% of gastric adenocarcinomas with lymph nade metastasis and 38% without lymph node metastasis (p<0.05) But, the PAI-1 expressian is not related to the number of lymph node metastasis and histopathologic grade. The results support the suggestion that PAI-1 may play a crucial role in metastatic progression of gastric adenocarcinoma and may serve to modulate proteolysis by PA.

2,740 View
12 Download

Immunoelectron Microscopic Localization of Transforming Growth Factor Beta1 and Bate2 in Human Gastric Carcinoma Cells: Young Euy Park; J Korean Cancer Assoc. 1996;28(3):411-418.

Abstract PDF: Immunohistochemical and immunoelectron microscopic studies were performed using specific monoclonal antibodies to transforming growth factor(TGF)-￥al, ￥a2 to determine their presence and cellular localization in human gastric adenocarinoma cells. Specific immunostaining was clearly detected in the cytoplasm of the neoplastic cells. The TGP-￥al, -￥a2 expression in the gastric adenocarcinoma is closely related to the depth of invasion, the degree of invasiveness and the presence of metastasis. In immuno-electron microscopy by 10nm gold particles, there are TGF￥a1, -￥a2 lacalized predominantly in the cytoplasm of the neoplastic cells. The gold particles were present within the secretory granules. The TGF-￥al, ￥a2 are not present in the cytoplasmic organells such as Golgi apparatus, mitachontria or rough endoplasmic reticulum.

2,433 View
12 Download

Immunohistochemical Analysis of Transforming Growth Factor - β Isoforms ( TGF-β1 , TGF-β2 , TGF-β3 ) Expression in Gastric Adenocarcinoma: Jae Hyung Yoo, Weon Sub Park, Mi Kyung Kim, Tae Jin Lee, Sang Jun Lee; J Korean Cancer Assoc. 1996;28(6):1040-1050.

Abstract PDF: Immunohistochemical studies using polyclonal antibodies to transforming growth factor beta isoforms(TGF-￥a1, TGF-￥a2 & TGF-￥a3), a multifunctional regulatory proteins which hoave effects on normal and transformed cells, were performed on 66 cases of gastric adenocarcinomas in order to analyze the relationship between expression of these isoforms in gastric cancer cells, adjacent mucosa of the cancer and normal control gastric mucosa. In addition to determine the relationship between expression of TGF-￥a isoforms and various clinicopathological states, including tumor location, histologic types, regional lymph node metastasis and depth of invasion of tumors were carried out. The positive staining reactivity was detected within the cytoplasm and on the cell membrane. The rate of TGF-￥a isoforms expression in gastric adenocarcinomas were 47% in TGF-￥a1, 83% in TGF-￥a2, and 27% in TGF-3, respectively. The adjacent gastric mucosa from adenocarcinomas and normal control mucosa were 40 % in TGF-￥a1, 40% and 55% in TGF-￥a2, 20% and 33% in TGF-￥a3, respectively. Among the TGF-￥a isoforms, TGF-￥a2 was strongly associated with histologic differentiation and regional lymph node metastasis. No significant association was found between expression of TGF-￥a isoforms and tumor location, histologic types and T-stages. From these results, we can postulate that the altered expression of TGF-￥a isoforms, especially TGF-￥a2, play an important role in histogenesis and gastric cancer progression and regional lymph node metastasis.

2,767 View
12 Download

Search