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2 "Endoplasmic reticulum stress"
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GRP78 Protein Expression as Prognostic Values in Neoadjuvant Chemoradiotherapy and Laparoscopic Surgery for Locally Advanced Rectal Cancer
Hee Yeon Lee, Ji-Han Jung, Hyun-Min Cho, Sung Hwan Kim, Kang-Moon Lee, Hyung-Jin Kim, Jong Hoon Lee, Byoung Yong Shim
Cancer Res Treat. 2015;47(4):804-812.   Published online January 30, 2015
DOI: https://doi.org/10.4143/crt.2014.121
AbstractAbstract PDFPubReaderePub
Purpose
We investigated the relationships between biomarkers related to endoplasmic reticulum stress proteins (glucose-regulated protein of molecular mass 78 [GRP78] and Cripto-1 [teratocarcinoma-derived growth factor 1 protein]), pathologic response, and prognosis in locally advanced rectal cancer. Materials and Methods All clinical stage II and III rectal cancer patients received 50.4 Gy over 5.5 weeks, plus 5- fluorouracil (400 mg/m2/day) and leucovorin (20 mg/m2/day) bolus on days 1 to 5 and 29 to 33, and surgery was performed at 7 to 10 weeks after completion of all therapies. Expression of GRP78 and Cripto-1 proteins was determined by immunohistochemistry and was assessed in 101 patients with rectal cancer treated with neoadjuvant chemoradiotherapy (CRT).
Results
High expression of GRP78 and Cripto-1 proteins was observed in 86 patients (85.1%) and 49 patients (48.5%), respectively. Low expression of GRP78 protein was associated with a significantly high rate of down staging (80.0% vs. 52.3%, respectively; p=0.046) and a significantly low rate of recurrence (0% vs. 33.7%, respectively; p=0.008) compared with high expression of GRP78 protein. Mean recurrence-free survival according to GRP78 expression could not be estimated because the low expression group did not develop recurrence events but showed a significant correlation with time to recurrence, based on the log rank method (p=0.007). GRP78 also showed correlation with overall survival, based on the log rank method (p=0.045). Conclusion GRP78 expression is a predictive and prognostic factor for down staging, recurrence, and survival in rectal cancer patients treated with 5-fluorouracil and leucovorin neoadjuvant CRT.

Citations

Citations to this article as recorded by  
  • Is GRP78 (Glucose-regulated protein 78) a prognostic biomarker in differents types of cancer? A systematic review and meta-analysis
    Natália Souza dos Santos, Douglas Rodrigues Gonçalves, Bianca Balbinot, Fernanda Visioli
    Pathology - Research and Practice.2023; 242: 154301.     CrossRef
  • GRP78 is Overexpressed in Non-small Cell Lung Cancer Tissues and is Associated with High VEGF Expression in Squamous Cell Carcinoma: A Pilot Study
    Maha Al-Keilani, Mohammad A. Alqudah, Basima Almomani, Moath M. Alrjoub, Batool A. Shhabat, Karem Alzoubi
    Current Cancer Drug Targets.2023; 23(10): 805.     CrossRef
  • GRP78 expression and prognostic significance in patients with pancreatic ductal adenocarcinoma treated with neoadjuvant therapy versus surgery first
    Yi Tat Tong, Hua Wang, Dongguang Wei, Laura R. Prakash, Michael Kim, Ching-Wei D. Tzeng, Jeffrey E. Lee, Asif Rashid, Eugene J. Koay, Robert A. Wolff, Anirban Maitra, Matthew HG. Katz, Huamin Wang
    Pancreatology.2021; 21(7): 1378.     CrossRef
  • Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma
    Tian Xia, Hui Tian, Kaiwen Zhang, Siyu Zhang, Wenhui Chen, Si Shi, Yiwen You
    BMC Cancer.2021;[Epub]     CrossRef
  • BCAP31, a cancer/testis antigen-like protein, can act as a probe for non-small-cell lung cancer metastasis
    Jing Wang, Dongbo Jiang, Zichao Li, Shuya Yang, Jiayi Zhou, Guanwen Zhang, Zixin Zhang, Yuanjie Sun, Zhipei Zhang, Xiaofei Li, Liang Tao, Jingqi Shi, Yuchen Lu, Lianhe Zheng, Chaojun Song, Kun Yang
    Scientific Reports.2020;[Epub]     CrossRef
  • Prognostic Value of Cripto-1 Expression in Non-Small-Cell Lung Cancer Patients: A Systematic Review and Meta-Analysis
    Yuanfeng Wei, Jinfang Jiang, Chengyan Wang, Hong Zou, Xihua Shen, Wei Jia, Shan Jin, Lu Zhang, Jianming Hu, Lan Yang, Lijuan Pang
    Biomarkers in Medicine.2020; 14(4): 317.     CrossRef
  • Interplay between endoplasmic reticulum stress and non-coding RNAs in cancer
    Tianming Zhao, Juan Du, Hui Zeng
    Journal of Hematology & Oncology.2020;[Epub]     CrossRef
  • Dual role of Endoplasmic Reticulum Stress-Mediated Unfolded Protein Response Signaling Pathway in Carcinogenesis
    Natalia Siwecka, Wioletta Rozpędek, Dariusz Pytel, Adam Wawrzynkiewicz, Adam Dziki, Łukasz Dziki, J. Alan Diehl, Ireneusz Majsterek
    International Journal of Molecular Sciences.2019; 20(18): 4354.     CrossRef
  • Clinical and Pathological Significance of ER Stress Marker (BiP/GRP78 and PERK) Expression in Malignant Melanoma
    Akira Shimizu, Kyoichi Kaira, Masahito Yasuda, Takayuki Asao, Osamu Ishikawa
    Pathology & Oncology Research.2017; 23(1): 111.     CrossRef
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    Xiao-bin Guo, Wan-le Ma, Li-juan Liu, Yu-ling Huang, Jing Wang, Li-hua Huang, Xiang-dong Peng, Ji-ye Yin, Jin-gao Li, Shao-jun Chen, Guo-ping Yang, Hui Wang, Cheng-xian Guo
    Acta Pharmacologica Sinica.2017; 38(4): 571.     CrossRef
  • The meta and bioinformatics analysis of GRP78 expression in gastric cancer
    Hua-Chuan Zheng, Bao-Cheng Gong, Shuang Zhao
    Oncotarget.2017; 8(42): 73017.     CrossRef
  • Endoplasmic reticulum ribosome-binding protein 1, RRBP1, promotes progression of colorectal cancer and predicts an unfavourable prognosis
    Y Pan, F Cao, A Guo, W Chang, X Chen, W Ma, X Gao, S Guo, C Fu, J Zhu
    British Journal of Cancer.2015; 113(5): 763.     CrossRef
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  • 15 Web of Science
  • 12 Crossref
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Blockade of Autophagy Aggravates Endoplasmic Reticulum Stress and Improves Paclitaxel Cytotoxicity in Human Cervical Cancer Cells
Li Xu, Jing-Hua Liu, Jing Zhang, Na Zhang, Zan-Hong Wang
Cancer Res Treat. 2015;47(2):313-321.   Published online September 11, 2014
DOI: https://doi.org/10.4143/crt.2013.222
AbstractAbstract PDFPubReaderePub
Purpose
Autophagy is one of the ways to degrade unfolded proteins after endoplasmic reticulum (ER) stress. The purpose of this study is to determine whether a blockade of autophagy leads to aggravated endoplasmic reticulum stress, which then induces cells apoptosis in HeLa cells treated with paclitaxel.
Materials and Methods
Autophagy activation and the proapoptotic effects were characterized using monodansylcadaverine labeling and Hoechest staining, respectively. A Western blot analysis was used to detect the expression of apoptotic and autophagy-related genes. A flow cytometry was used to assess the cell apoptosis ratio.
Results
Paclitaxel exposure induced the aggregation of autophagosomes in the cytoplasms of cervical cancer HeLa cells. The expression of Beclin 1 and LC3 II were upregulated, but p62 was downregulated, which suggests that autophagy was promoted by paclitaxel. On the other hand, the expression of GRP78 obviously increased, suggesting that ER stress was induced after paclitaxel treatment. The cell proliferation assay indicated that a knockdown of Beclin 1 sensitized HeLa cells to paclitaxel. Furthermore, paclitaxel-mediated apoptotic cell death was further potentiated by the pretreatment with autophagy inhibitor chloroquine or small interfering RNA against Beclin 1. These results suggest that an induction of autophagy by paclitaxel may induce cell survival rather than cell death in HeLa cells; moreover, inhibition of autophagy led to an aggravated ER stress and an induction of downstream apoptosis.
Conclusion
Our results reveal autophagy induced by paclitaxel conferred protection of tumor cells against apoptosis, and blockade of autophagy subsequently aggravated ER stress, enhancing the apoptosis associated with paclitaxel treatment in HeLa cells.

Citations

Citations to this article as recorded by  
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    Haitang Liao, Shuang Liu, Qiang Ma, He Huang, Arul Goel, Pedram Torabian, Chakrabhavi Dhananjaya Mohan, Chenyang Duan
    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research.2025; 1872(1): 119869.     CrossRef
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    Critical Reviews in Eukaryotic Gene Expression.2023; 33(6): 43.     CrossRef
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    Li Wang, Yueying Pan, Fan Yang, Xiaowang Guo, Junjun Peng, Xiaoyu Wang, Yukun Fang, Jing Chen, Xin Yi, Huabin Cao, Guoliang Hu
    Chemico-Biological Interactions.2022; 362: 109981.     CrossRef
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    Paulo Emilio Feuser, Ellen De Pieri, Maria Eduarda Oliveira, Arthur Poester Cordeiro, Rodrigo Cercena, Pedro Henrique Hermes de Araújo, Alexandre Gonçalves Dal Bó, Ricardo Andrez Machado-de-Ávila
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  • 137 Download
  • 39 Web of Science
  • 34 Crossref
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