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Gastrointestinal cancer
Establishing Patient-Derived Cancer Cell Cultures and Xenografts in Biliary Tract Cancer
Jihoon Kang, Ji-Young Lee, Sunmin Lee, Danbee Kim, Jinyeong Lim, Ha Ra Jun, Seyeon Jeon, Young-Ae Kim, Hye Seon Park, Kyu-pyo Kim, Sung-Min Chun, Hee Jin Lee, Changhoon Yoo
Cancer Res Treat. 2023;55(1):219-230.   Published online April 6, 2022
DOI: https://doi.org/10.4143/crt.2021.1166
AbstractAbstract PDFSupplementary MaterialPubReaderePub
Purpose
Biliary tract cancers (BTCs) are rare and show a dismal prognosis with limited treatment options. To improve our understanding of these heterogeneous tumors and develop effective therapeutic agents, suitable preclinical models reflecting diverse tumor characteristics are needed. We established and characterized new patient-derived cancer cell cultures and patient-derived xenograft (PDX) models using malignant ascites from five patients with BTC.
Materials and Methods
Five patient-derived cancer cell cultures and three PDX models derived from malignant ascites of five patients with BTC, AMCBTC-01, -02, -03, -04, and -05, were established. To characterize the models histogenetically and confirm whether characteristics of the primary tumor were maintained, targeted sequencing and histopathological comparison between primary tissue and xenograft tumors were performed.
Results
From malignant ascites of five BTC patients, five patient-derived cancer cell cultures (100% success rate), and three PDXs (60% success rate) were established. The morphological characteristics of three primary xenograft tumors were compared with those of matched primary tumors, and they displayed a similar morphology. The mutated genes in samples (models, primary tumor tissue, or both) from more than one patient were TP53 (n=2), KRAS (n=2), and STK11 (n=2). Overall, the pattern of commonly mutated genes in BTC cell cultures was different from that in commercially available BTC cell lines.
Conclusion
We successfully established the patient-derived cancer cell cultures and xenograft models derived from malignant ascites in BTC patients. These models accompanied by different genetic characteristics from commercially available models will help better understand BTC biology.

Citations

Citations to this article as recorded by  
  • The importance of preclinical models for cholangiocarcinoma drug discovery
    Felix J. Krendl, Florian Primavesi, Rupert Oberhuber, Daniel Neureiter, Matthias Ocker, Dino Bekric, Tobias Kiesslich, Christian Mayr
    Expert Opinion on Drug Discovery.2025; : 1.     CrossRef
  • Creation and Validation of Patient-Derived Cancer Model Using Peritoneal and Pleural Effusion in Patients with Advanced Ovarian Cancer: An Early Experience
    Ruri Nishie, Tomohito Tanaka, Kensuke Hirosuna, Shunsuke Miyamoto, Hikaru Murakami, Hiromitsu Tsuchihashi, Akihiko Toji, Shoko Ueda, Natsuko Morita, Sousuke Hashida, Atsushi Daimon, Shinichi Terada, Hiroshi Maruoka, Hiromi Konishi, Yuhei Kogata, Kohei Tan
    Journal of Clinical Medicine.2024; 13(9): 2718.     CrossRef
  • 5,949 View
  • 198 Download
  • 2 Web of Science
  • 2 Crossref
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What We Talk about When We Talk about Caregiving: The Distribution of Roles in Cancer Patient Caregiving in a Family-Oriented Culture
Ansuk Jeong, Dongwook Shin, Jong Hyock Park, Keeho Park
Cancer Res Treat. 2019;51(1):141-149.   Published online March 21, 2018
DOI: https://doi.org/10.4143/crt.2017.557
AbstractAbstract PDFSupplementary MaterialPubReaderePub
Purpose
When it comes to cancer care, the psychological well-being of family caregivers has gotten its deserved attention. However, the specific roles that the family caregivers take have not been examined as much. The current study aimed to investigate the distribution of family caregivers’ roles, particularly in a family-oriented culture, Korea.
Materials and Methods
A sample of 439 participants was recruited from 11 national and regional cancer centers in Korea. The participants who were 60 years old or above went through treatments for their gastric, colorectal, or lung cancer. The individual survey included questions regarding the family type, living arrangement, and the sources of support when it comes to their physical, emotional, financial, and decision-making needs.
Results
The responses from the participants showed that cancer caregiving is shared by multiple family caregivers; the major source of support for elderly cancer patients on diverse domains was their spouse; patients’ reliance on their daughter(s) increased for emotional support; and patients’ reliance on their son(s) stood out for financial support and decision-making support. Also, the older the patients were, the heavier their reliance was on the adult children, including sons, daughters, and daughters-in-law.
Conclusion
Future support programs for elderly cancer patients are suggested to involve multiple family caregivers to encourage effective and efficient intervention. Also, the limitations of the current study and the suggestions for future research are discussed.

Citations

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  • Effectiveness of Nursing Interventions for Patients With Cancer and their Family Members: A Systematic Review
    Cristina Alfaro-Díaz, Erla Kolbrun Svavarsdottir, Nuria Esandi, Marianne E. Klinke, Ana Canga-Armayor
    Journal of Family Nursing.2022; 28(2): 95.     CrossRef
  • Active Engagement, Protective Buffering, and Depressive Symptoms in Young-Midlife Couples Surviving Cancer: The Roles of Age and Sex
    Karen S. Lyons, Jessica R. Gorman, Brandon S. Larkin, Grace Duncan, Brandon Hayes-Lattin
    Frontiers in Psychology.2022;[Epub]     CrossRef
  • Exploring positive experiences of primary and secondary caregivers of older persons in resource-limited urban settings in Accra, Ghana
    Frank Kyei-Arthur, Samuel Nii Ardey Codjoe, Delali Margaret Badasu, Vijayaprasad Gopichandran
    PLOS ONE.2022; 17(4): e0266269.     CrossRef
  • Health practices in Europe towards families of older patients with cancer: a scoping review
    Hanne Konradsen, Anne Brødsgaard, Birte Østergaard, Erla Svavarsdóttir, Karin B. Dieperink, Lorenz Imhof, Marie Louise Luttik, Romy Mahrer‐Imhof, Cristina García‐Vivar
    Scandinavian Journal of Caring Sciences.2021; 35(2): 375.     CrossRef
  • Family Caregivers' Emotional Preparedness for Death is Distinct from Their Cognitive Prognostic Awareness for Cancer Patients
    Siew Tzuh, Wen-Cheng Chang, Wen-Chi Chou, Chia-Hsun Hsieh, Jen-Shi Chen, Fur-Hsing Wen
    Journal of Palliative Medicine.2021; 24(3): 405.     CrossRef
  • Exploring perceptions and practices of cancer care among caregivers and care recipients of breast cancer in India
    Shradha S. Parsekar, Ajay Bailey, Binu V. S., Suma Nair
    Psycho-Oncology.2020; 29(4): 737.     CrossRef
  • 8,563 View
  • 183 Download
  • 11 Web of Science
  • 10 Crossref
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Review Article
Biology of SNU Cell Lines
Ja-Lok Ku, Jae-Gahb Park
Cancer Res Treat. 2005;37(1):1-19.   Published online February 28, 2005
DOI: https://doi.org/10.4143/crt.2005.37.1.1
AbstractAbstract PDFPubReaderePub

SNU (Seoul National University) cell lines have been established from Korean cancer patients since 1982. Of these 109 cell lines have been characterized and reported, i.e., 17 colorectal carcinoma, 12 hepatocellular carcinoma, 11 gastric carcinoma, 12 uterine cervical carcinoma, 17 B-lymphoblastoid cell lines derived from cancer patients, 5 ovarian carcinoma, 3 malignant mixed Mllerian tumor, 6 laryngeal squamous cell carcinoma, 7 renal cell carcinoma, 9 brain tumor, 6 biliary tract, and 4 pancreatic carcinoma cell lines. These SNU cell lines have been distributed to biomedical researchers domestic and worldwide through the KCLB (Korean Cell Line Bank), and have proven to be of value in various scientific research fields. The characteristics of these cell lines have been reported in over 180 international journals by our laboratory and by many other researchers from 1987. In this paper, the cellular and molecular characteristics of SNU human cancer cell lines are summarized according to their genetic and epigenetic alterations and functional analysis.

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    Clinical Cancer Research.2016; 22(21): 5322.     CrossRef
  • Potential Role of CD133 Expression in the Susceptibility of Human Liver Cancer Stem-Like Cells to TRAIL
    Su-Hoon Lee, Suh-Kyung Hyun, Hak-Bong Kim, Chi-Dug Kang, Sun-Hee Kim
    Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics.2016; 24(6): 495.     CrossRef
  • GAGE12 mediates human gastric carcinoma growth and metastasis
    Eun Kyung Lee, Kyung‐A. Song, Ji‐Hye Chae, Kyoung‐Mee Kim, Seok‐Hyung Kim, Myung‐Soo Kang
    International Journal of Cancer.2015; 136(10): 2284.     CrossRef
  • Identification of Long-Range Epigenetic Silencing on Chromosome 15q25 and Its Clinical Implication in Gastric Cancer
    Jee-Youn Kang, Sang-Hyun Song, Jiyeon Yun, Mi-Seong Jeon, Yongjun Cha, Si-Hyun Lee, Hwang-Phill Kim, Eun-Goo Jeong, Sae-Won Han, Nam-Yun Cho, Myeong Cherl Kook, Gyeong Hoon Kang, Tae-You Kim
    The American Journal of Pathology.2015; 185(3): 666.     CrossRef
  • Targeting HIF1α Peri-operatively Increased Post-surgery Survival in a Tongue Cancer Animal Model
    Soon-Hyun Ahn, Joo Yeon Choi, Dong Wook Kim, Doh Young Lee, Eun-Hui Jeon, Woo-Jin Jeong, Jin Ho Paik
    Annals of Surgical Oncology.2015; 22(9): 3041.     CrossRef
  • Combined targeting of high‐mobility group box‐1 and interleukin‐8 to control micrometastasis potential in gastric cancer
    Hye Won Chung, Sunphil Jang, Hoguen Kim, Jong‐Baeck Lim
    International Journal of Cancer.2015; 137(7): 1598.     CrossRef
  • Evaluation of Lapatinib Powder-Entrapped Biodegradable Polymeric Microstructures Fabricated by X-Ray Lithography for a Targeted and Sustained Drug Delivery System
    Eun-Goo Jeong, Hyung Yoo, Byeonghwa Song, Hwang-Phill Kim, Sae-Won Han, Tae-You Kim, Dong-Il Cho
    Materials.2015; 8(2): 519.     CrossRef
  • Vaccinia-related kinase 1 promotes hepatocellular carcinoma by controlling the levels of cell cycle regulators associated with G1/S transition
    Namgyu Lee, Jung-Hee Kwon, Young Bae Kim, Seong-Hoon Kim, Sung Jin Park, Weiguang Xu, Hoe-Yune Jung, Kyong-Tai Kim, Hee Jung Wang, Kwan Yong Choi
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    Y Suh, C-H Yoon, R-K Kim, E-J Lim, Y S Oh, S-G Hwang, S An, G Yoon, M C Gye, J-M Yi, M-J Kim, S-J Lee
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Original Articles
Uptake of Ga-67 by Cultured Cells: Transferrin-dependent and Transferrin-independent Mechanisms
Myung Hee Sohn, Seok Tae Lim, Jae Yong Kwak, Chang Yeol Yim
J Korean Cancer Assoc. 2000;32(4):742-749.
AbstractAbstract PDF
PURPOSE
We determined whether the uptake of Ga-67 by cultured cells occur by both transferrin (Tf)-dependent and independent mechanisms and the mechanism and magnitude of its uptake may vary as the degree of expression of the transformed phenotype.
MATERIALS AND METHODS
Uptake of Ga-67 between the tansformed and untransformed cells was compared. Cells were incubated with Ga-67 in either the presence or absence of Tf and with complete medium containing Ga-67 after preincubating with anti-Tf receptor antibodies at 37oC in 8% CO2. Monolayers of cells were washed and trypsinized. Radioactivity and protein content of the samples were determined.
RESULTS
Uptake of Ga-67 by cultured cells occurred both in Tf-bound and ionic form and was increased with radioactivity and time. The magnitude for the uptake of Tf-bound form was approximately 3 and 6-fold greater than ionic form. In the presence of Tf, uptake of Ga-67 was 2-fold greater in the transformed cells. Conversely, In the absence of Tf, it was 1.5-fold greater in the untransformed cells. Regardless of blocking the Tf receptor by anti-Tf receptor antibodies, a significant amount of intracellular Ga-67 uptake was found.
CONCLUSION
Dual mechanisms exist for the uptake of Ga-67 by cultured cells. The primary important one was the Tf-dependent system. Tf-dependent and independent mechanisms and the magnitude operated oppositely in the transformed cells when compared to their untransformed counterpart.
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Effects of IFN - gamma on Spheroid and Raft Culture of Squamous Cell Carcinoma of the Head and Neck
Seung Ju Lee, Chun Dong Kim, Tae Young Koh, Keun Ho Chang, Chae Seo Rhee, Seong Jun Yoon, Sagn Goo Lee, Hyun Ju Lee, Kwang Hyun Kim
J Korean Cancer Assoc. 1998;30(3):573-582.
AbstractAbstract PDF
PURPOSE
To establish new in vitro model systems that better reflect in vivo condition, multicellular tumor spheroids(MTS) and raft culture were developed using cell lines of squamous cell carcinoma(SCCHN) of the head and neck. In these 3-dimensional systems, the expression of cell surface molecules which are important for modulation of physiology of tumor cells were studied with or without the treatment of interferon(IFN)-gamma.
MATERIALS AND METHODS
Four SCCHN cell lines were used for MTS and raft culture. The effects of interferon-gamma on SCCHN cells were examined by immunohistochemistry.
RESULTS
All cell lines formed MTS, but only Tu-138 showed a good stratification at the air-liquid interface in the raft culture system. Immunohistochemical studies of MTS using monoclonal antibodies revealed a strong staining for MHC class I, no staining for MHC-DR, a weak patch expression of ICAM-1 and a central strong staining for integrin a 6. Staining patterns were similar for the raft cultures except integrin a 6(intense full-thickness positivity). In both systems, IFN-gamma enhanced the expression of MHC-DR and ICAM-1. No significant change was found in the expression of MHC class I and integrin a 6.
CONCLUSIONS
MTS and raft culture system were established successfully from the SCCHN cell lines. IFN-gamma can modulate the surface molecules of tumor cells in the 3-dimensional culture systems.
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Establishment and Biological Carcterization of Human Colorectal Carcinoma Cell Lines Expressiong Carcinoembryonic Antigen
Jin Cheon Kim, In Chul Lee, Seon Ae Roh, Yoo Kyung Lee, Kyung Sin Kim, Kun Choon Park
J Korean Cancer Assoc. 1996;28(1):63-72.
AbstractAbstract PDF
For serum carcinoembryonic antigen (CEA) is not always elevated in recurrence or metastasis of colorectal carcinoma (CRC), activation of colorectal carcinoma cells by CEA may be selective. We have established and characterized two CEA-expressing colorectal carcinoma cell lines which were analysed in relation to the clinical course. Two cell lines originated from rectal carcinoma were cultured in DME enhanced media and subcultured by 40 and 55 times respectively. AMC 4 grew in an anchrage-independent with delayed cell doubling time (93 hrs), while AMC 5 did in an anchorage-dependent with normal doubling time (43 hrs). Tumorigenesis revealed infiltrative moderate-differentiated adenocarcinoma in AMC 4 and expansile well-differentiated adenocarcinoma in AMC 5. Karyotyping by Trypsin-Giemsa banding showed 47, XY, 21(+ ), del (2q, 5q) and 46, XY, 5(+ ), 20(+ ), 12(- ) respectively. Both cell lines were aneuploid. AMC 5 expressed large amount of 55 kD NCA as well as 180 KD CEA, but AMC 4 did traces of them by indirect immunofluorescence and immunoblot. AMC 5 cells bound optimally to solid-phase type IV collegen, laminin, and CEA by 18.7¡¾ 0.4, 21.4 ¡¾ 0.6 and 17.7¡¾0.4, while AMC 4 cells bound less. The patient of AMC 4 showed liver metastasis on 6 months after curative surgery and AMC 5 did no evidence of recurrence. In conclusion, biological characteristics of two CEA-expressing CRC cell lines seemed to be consistent with their clinical course.
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