Cancer Research and Treatment

Original Articles

Hydrogen Peroxide Producing Lactobacilli in Women with Cervical Neoplasia: Ho Sun Choi, Ki Min Kim, Chol Hong Kim, Seok Mo Kim, Jong Seok Oh; Cancer Res Treat. 2006;38(2):108-111. Published online April 30, 2006; DOI: https://doi.org/10.4143/crt.2006.38.2.108

Purpose

It is well known that human papillomavirus (HPV) is the main cause of cervical neoplasia, and hydrogen peroxide-producing lactobacilli are the most important microorganisms for maintaining the balance of the vaginal ecosystem. The purpose of our study was to investigate the relationship of hydrogen peroxide-producing lactobacilli, cervical neoplasia and high-risk HPV.

Materials and Methods

We enrolled 1138 women with abnormal cervical smears or cervicograms who were referred to the department of Obstetrics and Gynecology at Chonnam National University Medical School. In all of them, 1,138 vaginal swabs were collected for the qualitative assay of hydrogen peroxide producing lactobacilli and 150 cervical swabs were used for the HPV hybrid capture II test without regard to the subjects' pregnancy status. In the non-pregnant women, 880 cervical biopsies and/or loop electrosurgical excision procedures were performed for making the histological diagnosis.

Results

There was no significant difference not only between the distribution of H₂O₂ producing lactobacilli and the cervical histology, but also between the distribution of H₂O₂ producing lactobacilli and the positivity for high-risk HPV.

Conclusions

Both cervical neoplasia and high-risk HPV may not be influenced by the existence of hydrogen peroxide producing lactobacilli in the vagina.

Citations

Citations to this article as recorded by

Unravelling the Biological Interplay Between Genital HPV Infection and Cervicovaginal Microbiota in Sub-Saharan Africa: Implications for Cervical (Pre)cancer Prevention
Harris Onywera, Zizipho Z. A. Mbulawa, Adrian Brink, Anna-Lise Williamson, Lamech M. Mwapagha
Venereology.2024; 3(4): 211. CrossRef
Antimicrobial activity of Lactobacillus against microbial flora of cervicovaginal infections
Subramanyam Dasari, Raju Naidu Devanaboyaina Shouri, Rajendra Wudayagiri, Lokanatha Valluru
Asian Pacific Journal of Tropical Disease.2014; 4(1): 18. CrossRef
PCR‐based identification of eight lactobacillus species and 18 hr‐HPV genotypes in fixed cervical samples of south african women at risk of HIV and BV
Joke A.M. Dols, Gregor Reid, Remco Kort, Frank H.J. Schuren, Hugo Tempelman, Tj. Romke Bontekoe, Hans Korporaal, E.M. Van der Veer, Pieter W. Smit, Mathilde E. Boon
Diagnostic Cytopathology.2012; 40(6): 472. CrossRef

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Polymorphisms of p53, p21 and IRF-1 and Cervical Cancer Susceptibility in Korean Women: Sung Jong Lee, Sung Eun Namkoong, Won Chul Lee, Jae Woong Sul, Sun Ha Jee, Youn Kyoung You, Jong Eun Lee, Jong Sup Park; Cancer Res Treat. 2002;34(5):357-364. Published online October 31, 2002; DOI: https://doi.org/10.4143/crt.2002.34.5.357

Abstract PDF

PURPOSE
The aim of this study was to identify gene- gene and gene-environmental factor on cervical carcinogenesis in Korean women.
MATERIALS AND METHODS
We evaluated 185 women patients who had cervical cancer with 345 normal control healthy women. The single nucleotide polymorphisms (SNPs) of the p53 codon 72, the p21 codon 31 and the IRF-1 intron 6 were evaluated from extracted DNA of peripheral blood with an automatic DNA sequencer. The difference of each SNP, gene-gene and gene-environmental interaction between normal controls and patients, were evaluated in an adjusted environmental background.
RESULTS
With regard to environmental factors, the cervical cancer increased in the women with a lower level of education, a younger age at first sexual intercourse and with the increased number of children borne. The women who had p53 (Arg/Arg), IRF-1 (T/T) and an education of less than 6 years showed a 14.7 fold increased risk of cervical cancer than those women who had p53 (~Pro), IRF-1 (~C) and an education of more than 15 years. The women who had p53 (Arg/Arg), p21 (Ser/Ser) and more than 3 children showed a 6.4 fold increased risk of cervical cancer than those women who had p53 (~Pro), p21 (~Arg) and had borne no child. The women who had p53 (Arg/Arg), IRF-1 (T/T) and had experience of first sexual intercourse before the age of 22-years showed a 5.5 fold increased risk of cervical cancer than those women who had p53 (~Pro), IRF-1 (~C) and had experience of first sexual intercourse after the age of 26-years.
CONCLUSION
We found that the level of education, the age at first intercourse, and the number of children borne, were independent risk factors in cervical carcinogenesis. The specific combination of p53, p21 and IRF-1 gene-gene and gene-environmental interactions were significantly noted in the cervical carcinogenesis of Korean women.

Citations

Citations to this article as recorded by

Distinctive cell cycle regulatory protein profiles by adenovirus delivery of p53 in human papillomavirus-associated cancer cells
H.-S. JIN, S.-M. BAE, Y.-W. KIM, J.-M. LEE, S.-E. NAMKOONG, B.-D. HAN, Y.-J. LEE, C.-K. KIM, H.-J. CHUN, W.-S. AHN
International Journal of Gynecological Cancer.2006; 16(2): 698. CrossRef
Cell Cycle Regulatory Protein Expression Profiles by Adenovirus p53 Infection in Human Papilloma Virus-associated Cervical Cancer Cells
Yong-Seok Lee, Su-Mi Bae, Sun-Young Kwak, Dong-Chun Park, Yong-Wook Kim, Soo-Young Hur, Eun-Kyung Park, Byoung-Don Han, Young-Joo Lee, Chong-Kook Kim, Do Kang Kim, Woong-Shick Ahn
Cancer Research and Treatment.2006; 38(3): 168. CrossRef
Cellular process classification of human papillomavirus-16-positive SiHa cervical carcinoma cell using Gene Ontology
W. S. Ahn, M.-J. Seo, S. M. Bae, J. M. Lee, S. E. Namkoong, C. K. Kim, Y.-W. Kim
International Journal of Gynecological Cancer.2005; 15(1): 94. CrossRef

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Immunologic and Oncogenic Study on the Cervical Neoplasia: Eun Yeong Seol, Sung Chul Lim; J Korean Cancer Assoc. 1996;28(6):1071-1083.

Abstract PDF: Immune response commonly is cited as a determining factor in the development or clearance of various neoplasias, but the immunobiology of neoplastic progression is poorly understood. The cervix has the largest concentration of lymphocytes found in the female genital tract and as a component of the common mucosal immune system guards against ascending infection from the microbial-laiden vagina, but cervical immunocytes have not been well characterized in the neoplasia itself. The objective of this study is to characterize the subpopulations of lymphocytes that infiltrate various grades of cervical neoplasia including metaplasia to invasive carcinoma. To establish the correlation I examined 60 cases of uterine cervix which were divided 4 categories according to the Bethesda Classification system; normal cervix(15), low grade squamous intraepithelial lesions(SILs)(15), high grade SILs(15), and invasive squamous cell carcinomas(15 cases). The degrees of T-lymphocyte infiltration were assessed after immunohistochemical staining by UCHL1, and compared to stainability of aberrant p53 and bcl-2 protein. There were significant increasing number and proportion of T-cells of invaisve cancers compared with that of preinvasive lesions. Aberrant p53 expression of invasive cancer was a little more intensive than that of low grade and high grade SILs, and there was no correlation between T-cell infiltration and aberrant p53 and/or bcl-2 expression. Bcl-2 was expressed in most of basal layer and some cases of parabasal layer of low and high grade SILs, and some minute foci of invasive cancer. In the adjacent areas of SILs and invasive lesions strong bcl-2 expression was noted in parabasal, intermediate or superficial cells case by case. In conclusion, the UCHLl-positive T cell infiltrate far exceeded in the invasive, but not in the preinvasive lesions, a finding that suggests that T cells are recruited preferentially to cervical lesions with progression to invasion. It is suggested that the T cell recruitment is not related to p53 or bc1-2, but the unknown third factors, and the bcl-2 overexpression is involved in the early step of epithelial neoplastic transforrnation and followed by recurrent overexposure to various oncogenic factors.

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