Cancer Research and Treatment

Original Article

Time-course Transcriptional Profiling of Human Amniotic Fluid-derived Stem Cells Using Microarray: Yong Wook Kim, Hyun-Jung Kim, Su-Mi Bae, Young Jae Kim, Jong-Chul Shin, Heung-Jae Chun, Jong-Won Rhie, Jiyoung Kim, Haekwon Kim, Woong Shick Ahn; Cancer Res Treat. 2010;42(2):82-94. Published online June 30, 2010; DOI: https://doi.org/10.4143/crt.2010.42.2.82

Purpose

To maintain the homeostasis of stem cells and prevent their ability to initiate tumorigenesis, it is important to identify and modify factors that prevent or accelerate stem cell senescence. We used microarrays to attempt to identify such factors in human amniotic fluid (HAF)-derived stem cells.

Materials and Methods

To identify gene expression changes over a time course, we compared gene expression profiles of HAF-derived stem cells in different passages (1^st, 2^nd, 4^th, 6^th, 8^th, and 10^th) using a Sentrix Human illumina microarray.

Results

Of the 25,804 genes in the microarray chip, 1,970 showed an over 2-fold change relative to the control (the 1^st passage)-either upregulated or downregulated. Quantitative real-time PCR validated the microarray data for selected genes: markedly increased genes were CXCL12, cadherin 6 (CDH6), and folate receptor 3 (FOLR3). Downregulated genes included cyclin D2, keratin 8, insulin-like growth factor 2 (IGF2), natriuretic peptide precursor B (NPPB) and cellular retinoic acid binding protein 2 (CRABP2). The expression pattern of the selected genes was consistent with the microarray data except for CXCL12 and IGF2. Interestingly, the expression of NPPB was dramatically downregulated along the time course; it was almost completely shut-down by the 10^th passage. In contrast, FOLR3 mRNA expression was dramatically increased.

Conclusion

Taken together, although a function for NPPB and FOLR3 in stem cell senescence has not been reported, our results strongly suggest that NPPB and/or FOLR3 play a significant role in the regulation of stem cell senescence.

Citations

Citations to this article as recorded by

Recent Advances in Folates and Autoantibodies against Folate Receptors in Early Pregnancy and Miscarriage
Xue-Yun Qin, Si-Yao Ha, Lu Chen, Tao Zhang, Ming-Qing Li
Nutrients.2023; 15(23): 4882. CrossRef
Cancer Stem Cells: Emergent Nature of Tumor Emergency
Yaroslav R. Efremov, Anastasia S. Proskurina, Ekaterina A. Potter, Evgenia V. Dolgova, Oksana V. Efremova, Oleg S. Taranov, Aleksandr A. Ostanin, Elena R. Chernykh, Nikolay A. Kolchanov, Sergey S. Bogachev
Frontiers in Genetics.2018;[Epub] CrossRef
Systematic identification of an integrative network module during senescence from time-series gene expression
Chihyun Park, So Jeong Yun, Sung Jin Ryu, Soyoung Lee, Young-Sam Lee, Youngmi Yoon, Sang Chul Park
BMC Systems Biology.2017;[Epub] CrossRef
Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections
Lucas-Sebastian Spitzhorn, Md Shaifur Rahman, Laura Schwindt, Huyen-Tran Ho, Wasco Wruck, Martina Bohndorf, Silke Wehrmeyer, Audrey Ncube, Ines Beyer, Carsten Hagenbeck, Percy Balan, Tanja Fehm, James Adjaye
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Scavenger receptor class A member 5 ( SCARA5 ) and suprabasin ( SBSN ) are hub genes of coexpression network modules associated with peripheral vein graft patency
Richard D. Kenagy, Mete Civelek, Shinsuke Kikuchi, Lihua Chen, Anthony Grieff, Michael Sobel, Aldons J. Lusis, Alexander W. Clowes
Journal of Vascular Surgery.2016; 64(1): 202. CrossRef
Amniotic Fluid-Derived Stem Cells (AFSC) and Their Application in Cell Therapy and Tissue Engineering
Syeda Zahra Anum, Seyed Raheel Muzavir, Ahmad Hassan, Amir Ali Khan, Aftab Ahmad
Razavi International Journal of Medicine.2015;[Epub] CrossRef
Simvastatin Modulates Mesenchymal Stromal Cell Proliferation and Gene Expression
Dalila Lucíola Zanette, Julio Cesar Cetrulo Lorenzi, Rodrigo Alexandre Panepucci, Patricia Vianna Bonini Palma, Daiane Fernanda dos Santos, Karen Lima Prata, Wilson Araújo Silva, Benedetta Bussolati
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Senescence and longevity in amniotic fluid derived cells
Z. Chen, A. Jadhav, F. Wang, M. Perle, R. Basch, B. K. Young
Stem Cell Discovery.2013; 03(01): 47. CrossRef
Amniotic Fluid and Amniotic Membrane Stem Cells: Marker Discovery
Maria G. Roubelakis, Ourania Trohatou, Nicholas P. Anagnou
Stem Cells International.2012; 2012: 1. CrossRef
Applications of Amniotic Membrane and Fluid in Stem Cell Biology and Regenerative Medicine
Kerry Rennie, Andrée Gruslin, Markus Hengstschläger, Duanqing Pei, Jinglei Cai, Toshio Nikaido, Mahmud Bani-Yaghoub
Stem Cells International.2012; 2012: 1. CrossRef

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Retraction

Retraction: Comparison of As2O3 and As4O6 in the Detection of SiHa Cervical Cancer Cell G rowth Inhibition Pathway: Yong Wook Kim, Su Mi Bae, Keun Ho Lee, Joon Mo Lee, Sung Eun Namkoong, Insu P. Lee, Chong Kook Kim, Jeong-Sun Seo, Jeong-Im Sin, Yong-Wan Kim, Woong Shick Ahn; Cancer Res Treat. 2007;39(1):47-47. Published online March 31, 2007; DOI: https://doi.org/10.4143/crt.2007.39.1.47; Retracts: Cancer Res Treat 2004;36(4):255

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Original Articles

Activity of Green Tea Polyphenol Epigallocatechin-3-gallate Against Ovarian Carcinoma Cell Lines: Yong Wook Kim, Su Mi Bae, Joon Mo Lee, Sung Eun Namkoong, Sei Jun Han, Byoung Rai Lee, Insu P. Lee, Sang Hee Kim, Young Joo Lee, Chong Kook Kim, Yong-Wan Kim, Woong Shick Ahn; Cancer Res Treat. 2004;36(5):315-323. Published online October 31, 2004; DOI: https://doi.org/10.4143/crt.2004.36.5.315

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Purpose

A constituent of green tea, (-)-epigallocatechin-3-gallate (EGCG), is known to possess anti-cancer properties. In this study, the time-course of the anticancer effects of EGCG on human ovarian cancer cells were investigated to provide insights into the molecular-level understanding of the growth suppression mechanism involved in EGCG-mediated apoptosis and cell cycle arrest.

Materials and Methods

Three human ovarian cancer cell lines (p53 negative, SKOV-3 cells; mutant type p53, OVCAR-3 cells; and wild type p53, PA-1 cells) were used. The effect of EGCG treatment was studied via a cell count assay, cell cycle analysis, FACS, Western blot and macroarray assay.

Results

EGCG exerts a significant role in suppressing ovarian cancer cell growth, showed dose dependent growth inhibitory effects in each cell line and induced apoptosis and cell cycle arrest. The cell cycle was arrested at the G1 phase by EGCG in SKOV-3 and OVCAR-3 cells.

In contrast, the cell cycle was arrested in the G1/S phase in PA-1 cells. EGCG differentially regulated the expression of genes and proteins (Bax, p21, Retinoblastoma, cyclin D1, CDK4 and Bcl-X_L) more than 2 fold, showing a possible gene regulatory role for EGCG. The continual expression in p21WAF1 suggests that EGCG acts in the same way with p53 proteins to facilitate apoptosis after EGCG treatment. Bax, PCNA and Bcl-X are also important in EGCG-mediated apoptosis. In contrast, CDK4 and Rb are not important in ovarian cancer cell growth inhibition.

Conclusion

EGCG can inhibit ovarian cancer cell growth through the induction of apoptosis and cell cycle arrest, as well as in the regulation of cell cycle related proteins. Therefore, EGCG-mediated apoptosis could be applied to an advanced strategy in the development of a potential drug against ovarian cancer.

Citations

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Anna Markowska, Michał Antoszczak, Janina Markowska, Adam Huczyński
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Yuanyuan Zhao, Lichao Lu, Xingying Chen, Qiaozhi Yin
Pharmacological Research.2025; 215: 107729. CrossRef
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Manzar Alam, Sabeeha Ali, Ghulam Md. Ashraf, Anwar L. Bilgrami, Dharmendra Kumar Yadav, Md. Imtaiyaz Hassan
Food Chemistry.2022; 379: 132135. CrossRef
Synergistic effects of green tea extract and paclitaxel in the induction of mitochondrial apoptosis in ovarian cancer cell lines
Mohammad Panji, Vahideh Behmard, Zahra Zare, Monireh Malekpour, Hasan Nejadbiglari, Saeede Yavari, Tina Nayerpour dizaj, Azadeh Safaeian, Ali Bakhshi, Omid Abazari, Mojtaba Abbasi, Parisa Khanicheragh, Maryam Shabanzadeh
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Ana Margarida Teixeira, Clara Sousa
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Estrogenic biological activity and underlying molecular mechanisms of green tea constituents
Ryoiti Kiyama
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Potential Therapeutic Targets of Epigallocatechin Gallate (EGCG), the Most Abundant Catechin in Green Tea, and Its Role in the Therapy of Various Types of Cancer
Saleh A. Almatroodi, Ahmad Almatroudi, Amjad Ali Khan, Fahad A. Alhumaydhi, Mohammed A. Alsahli, Arshad Husain Rahmani
Molecules.2020; 25(14): 3146. CrossRef
The Potential Roles of Epigallocatechin-3-Gallate in the Treatment of Ovarian Cancer: Current State of Knowledge

Sabrina Bimonte, Marco Cascella
Drug Design, Development and Therapy.2020; Volume 14: 4245. CrossRef
Bioinformatics Analysis on Molecular Mechanism of Green Tea Compound Epigallocatechin‐3‐Gallate Against Ovarian Cancer
S Xinqiang, Z Mu, C Lei, LY Mun
Clinical and Translational Science.2017; 10(4): 302. CrossRef
Pharmacological profile of green tea and its polyphenols: a review
Sumit Bansal, Navneet Syan, Pooja Mathur, Shivani Choudhary
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Salvatore Chirumbolo
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Dominique Trudel, David P. Labbé, Isabelle Bairati, Vincent Fradet, Laurent Bazinet, Bernard Têtu
Gynecologic Oncology.2012; 126(3): 491. CrossRef
Tea Consumption and Epithelial Ovarian Cancer Risk: A Systematic Review of Observational Studies
Sarah J. Oppeneer, Kim Robien
Nutrition and Cancer.2011; 63(6): 817. CrossRef

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Comparison of As₂O₃ and As₄O₆ in the Detection of SiHa Cervical Cancer Cell Growth Inhibition Pathway: Yong Wook Kim, Su Mi Bae, Keun Ho Lee, Joon Mo Lee, Sung Eun Namkoong, Insu P. Lee, Chong Kook Kim, Jeong-Sun Seo, Jeong-Im Sin, Yong-Wan Kim, Woong Shick Ahn; Cancer Res Treat. 2004;36(4):255-262. Published online August 31, 2004; DOI: https://doi.org/10.4143/crt.2004.36.4.255; Retraction in: Cancer Res Treat 2007;39(1):47

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Expression Profiling of the Cellular Processes in Uterine Leiomyomas: Omic Approaches and IGF-2 Association with Leiomyosarcomas: Su Mi Bae, Yong-Wan Kim, Joon Mo Lee, Sung Eun Namkoong, Chong Kook Kim, Woong Shick Ahn; Cancer Res Treat. 2004;36(1):31-42. Published online February 29, 2004; DOI: https://doi.org/10.4143/crt.2004.36.1.31

Abstract PDF PubReader ePub

Purpose

This study utilized both cDNA microarray and 2D protein gel electrophoresis technology to investigate the multiple interactions of the genes and proteins involved in the pathophysiology of uterine leiomyomas. Also, Gene Ontology (GO) analysis was used to systematically characterize the global expression profiles, which were found to correlate with the leiomyosarcomas.

Materials and Methods

The uterine leiomyoma biopsies were obtained from patients in the Department of Obstetrics and Gynecology, The Catholic University of Korea. Differentially expressed transcriptome and proteome, in 6 paired leiomyoma and normal myometrium, were profiled. The total RNAs from the leiomyoma and normal myometrium were labeled with Cy5 and Cy3. All specimens were punch-biopsy-obtained, and frozen in liquid nitrogen.

Results

Screening of up to 17,000 genes identified 71 that were either up-regulated or down-regulated (21 and 50, respectively). The gene expression profiles were classified into 420 mutually dependent functional sets, resulting in 611 cellular processes, according to the gene ontology. Also, the protein analysis, using 2D gel electrophoresis, identified 33 proteins (17 up-regulated and 16 down-regulated) with more than 500 total spots, which were classified into 302 cellular processes. O f these functional profilings, transcriptomes and proteoms down-regulations were shown in the cell adhesion, cell m otility, organogenesis, enzyme regulator, structural molecule activity and responses to external stimulus functional activities, which are supposed to play important roles in the pathophysiology. In contrast, up-regulation was only shown in the nucleic acid binding activity. The CDKN2A, ADH1A, DCX, IGF2, CRABP2 and KIF5C were found to increase the reliability of this study, and correlate with the leiomyosarcomas.

Conclusion

Potentially significant pathogenetic cellular processes showed that down-regulated functional profiling has an important impact on the discovery of the pathogenic pathways in leiomyomas and leiomyosarcomas. GO analysis can also overcome the complexity of the expression profiles of cDNA microarrays and 2D protein analyses, via a cellular process level approach. Thereby, a valuable prognostic candidate gene, with real relevance to disease-specific pathogenesis, can be found at cellular process levels.

Citations

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H3K4me3 mediates uterine leiomyoma pathogenesis via neuronal processes, synapsis components, proliferation, and Wnt/β-catenin and TGF-β pathways
María Cristina Carbajo-García, Elena Juarez-Barber, Marina Segura-Benítez, Amparo Faus, Alexandra Trelis, Javier Monleón, Greta Carmona-Antoñanzas, Antonio Pellicer, James M. Flanagan, Hortensia Ferrero
Reproductive Biology and Endocrinology.2023;[Epub] CrossRef
Differential Expression of MED12-Associated Coding RNA Transcripts in Uterine Leiomyomas
Tsai-Der Chuang, Jianjun Gao, Derek Quintanilla, Hayden McSwiggin, Drake Boos, Wei Yan, Omid Khorram
International Journal of Molecular Sciences.2023; 24(4): 3742. CrossRef
Analysis of JAM-A T>C (rs790056) and LFA-1 2120 G>C (rs2230433) gene variations in uterine leiomyoma
Özge KÖMÜRCÜ KARUSERCİ, Esra GÜZEL TANOĞLU, Halime Hanım PENÇE, Mete Gürol UĞUR
Anatolian Current Medical Journal.2021; 3(1): 48. CrossRef
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Shubha Gururaja Rao, Neel J. Patel, Harpreet Singh
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Leiomyosarcoma: A rare soft tissue cancer arising from multiple organs
Zorawar Singh
Journal of Cancer Research and Practice.2018; 5(1): 1. CrossRef
Integrated analysis reveals candidate mRNA and their potential roles in uterine leiomyomas
Liping Xia, Yan Liu, Yan Fu, Shengyi Dongye, Dewei Wang
Journal of Obstetrics and Gynaecology Research.2017; 43(1): 149. CrossRef
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Qiong Zou, Zhulin Yang, Daiqiang Li, Ziru Liu, Yuan yuan
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Biochimica et Biophysica Acta (BBA) - Biomembranes.2015; 1848(10): 2523. CrossRef
Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis
BLENDI URA, FEDERICA SCRIMIN, FABRIZIO ZANCONATI, GIORGIO ARRIGONI, LORENZO MONASTA, ANDREA ROMANO, RUBINA BANCO, MARINA ZWEYER, DANIELA MILANI, GIUSEPPE RICCI
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Photogem Induces Necrosis in Various Uterine Cervical Cancer Cell Lines by PDT: Su Mi Bae, Seung Won Huh, Eun Kyung Park, Keun Ho Lee, Joon Mo Lee, Sung Eun Namkoong, Sei Jun Han, Chong Kook Kim, Jong Ki Kim, Yong Wan Kim, Woong Shick Ahn; Cancer Res Treat. 2003;35(6):549-556. Published online December 31, 2003; DOI: https://doi.org/10.4143/crt.2003.35.6.549

Abstract PDF

PURPOSE
In order to elucidate the antitumor effect of photodynamic therapy (PDT), using a derivative of the photosensitizing agent hematoporphyrin (Photogem) and a diode laser, the cell death of uterine cancer cell lines (CaSki, HT3, HeLa, and SKOV-3), and mice transplanted with TC-1 lung cancer cells, were evaluated. MATERIALS AND METHODS: The morphological changes, MTT assay, flow cytometry, cytotoxicity and tumor growth inhibition study were evaluated at various time intervals after the PDT.
RESULTS
The results showed that the survival rates of each cell line decreased with time and dose response after performing the PDT. Also, the PDT-induced damage of cancer cells was almost entirely confined to necrosis of the tumor cells in the early time courses. The irradiation of CaSki cells in the presence of Photogem induced plasma membrane disruption and cell shrinkage, indicating the plasma membrane as the main target for Photogem. In the in vivo experiment, significantly longer survival and a significantly smaller tumor size were seen over the time courses of the Photogem with irradiation compared to the untreated control groups; resorption of the tumor was also observed after the PDT treatment. CONCLUSION: Collectively, our results indicated that Photogem possesses anti-tumor effects, and necrosis-like death, with plasma membrane damage, was postulated to be the principal mechanism of the antitumor effect of the PDT using Photogem.

Citations

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Therapeutic effects of systemic photodynamic therapy in a leukemia animal model using A20 cells
Lan Ying Wen, Su-Mi Bae, Heung-Jae Chun, Kye-Shin Park, Woong Shick Ahn
Lasers in Medical Science.2012; 27(2): 445. CrossRef
Shift from Apoptotic to Necrotic Cell Death during Human Papillomavirus-induced Transformation of Keratinocytes
Nataly Kravchenko-Balasha, Sarit Mizrachy-Schwartz, Shoshana Klein, Alexander Levitzki
Journal of Biological Chemistry.2009; 284(17): 11717. CrossRef
Photodynamic Effects of Radachlorin® on Cervical Cancer Cells
Su-Mi Bae, Yong-Wook Kim, Joon-Mo Lee, Sung-Eun Namkoong, Sei-Jun Han, Jong-Ki Kim, Chang-Hee Lee, Heung-Jae Chun, Hyun-Sun Jin, Woong-Shick Ahn
Cancer Research and Treatment.2004; 36(6): 389. CrossRef

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cDNA Microarray Analysis of Gene Expression Profiles Associated with Cervical Cancer: Joo Hee Yoon, Joon Mo Lee, Sung Eun Namkoong, Su Mi Bae, Yong Wan Kim, Sei Jun Han, Young Lae Cho, Gye Hyun Nam, Chong Kook Kim, Jeong Sun Seo, Woong Shick Ahn; Cancer Res Treat. 2003;35(5):451-459. Published online October 31, 2003; DOI: https://doi.org/10.4143/crt.2003.35.5.451

Abstract PDF

PURPOSE
The molecular pathology of cervical cancers associated with human papillomavirus infection is presently unclear. In an effort to clarify this issue, the gene expression profiles and pathogenic cellular processes of cervical cancer lesions were investigated. MATERIALS AND METHODS: Cervical cancer biopsies were obtained from patients at the Department of Obstetrics and Gynecology, The Catholic University of Korea. The disease status was assigned according to the International Federation of Gynecology and Obstetrics. The tissue samples of 11 patients (invasive cancer stage Ib- IIIa) were investigated by a cDNA microarray of 4, 700 genes, hierarchical clustering and the Gene Ontology (GO). Total RNA from cervical cancer and non-lesional tissues were labeled with Cy5 and Cy3. The HaCaT human epithelial keratinocyte cell line was used as a negative control cell. The stages of invasive cancer were Ib to IIIb. All specimens were obtained by punch-biopsies and frozen in liquid nitrogen until required. RESULTS: 74 genes, showing more than a 2 fold difference in their expressions, were identified in at least 8 of the 11 patients. Of these genes, 33 were up-regulated and 41 were down-regulated. The gene expression profiles were classified into 345 mutually dependent function sets, resulting in 611 cellular processes according to their GO. The GO analysis showed that cervical carcinogenesis underwent complete down-regulation of cell death, protein biosynthesis and nucleic acid metabolism. The genes related to nucleic acid binding and structural molecule activity were also significantly down-regulated. In contrast, significant up-regulation was shown in the skeletal development, immune response and extracellular activity. CONCLUSION: These data are suggestive of potentially significant pathogenetic cellular processes, and showed that the down-regulated functional profiling has an important impact on the discovery of pathogenic pathways in cervical carcinogenesis. GO analysis can also overcome the complexity of the expression profiles of the cDNA microarray via a cellular process level approach. Thereby, a valuable prognostic candidate gene, with real relevance to disease-specific pathogenesis, can be found at the cellular process levels.

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The Ukrainian Biochemical Journal.2016; 88(6): 52. CrossRef
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Simon A. Rudge, Michael J.O. Wakelam
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Ju Youn Park, Jae Ho Chang, Myong Jo Kim, Ju Sung Kim, Soo-Ki Kim
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New Approaches to Functional Process Discovery in HPV 16-Associated Cervical Cancer Cells by Gene Ontology: Yong Wan Kim, Min Je Suh, Jin Sik Bae, Su Mi Bae, Joo Hee Yoon, Soo Young Hur, Jae Hoon Kim, Duck Young Ro, Joon Mo Lee, Sung Eun Namkoong, Chong Kook Kim, Woong Shick Ahn; Cancer Res Treat. 2003;35(4):304-313. Published online August 31, 2003; DOI: https://doi.org/10.4143/crt.2003.35.4.304

Abstract PDF

No abstract available.

Citations

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BAG3 down-modulation sensitizes HPV18+ HeLa cells to PEITC-induced apoptosis and restores p53
Roberta Cotugno, Anna Basile, Elena Romano, Dario Gallotta, Maria Antonietta Belisario
Cancer Letters.2014; 354(2): 263. CrossRef
Expression Profiling of the Cellular Processes in Uterine Leiomyomas: Omic Approaches and IGF-2 Association with Leiomyosarcomas
Su Mi Bae, Yong-Wan Kim, Joon Mo Lee, Sung Eun Namkoong, Chong Kook Kim, Woong Shick Ahn
Cancer Research and Treatment.2004; 36(1): 31. CrossRef

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Cell-Specific Growth Inhibition of Human Cervical Cancer Cell by Recombinant Adenovirus p53 in vitro and in vivo: Su Mi Bae, Yong Wook Kim, Joo Hee Yoon, Jin Young Yoo, Young Seok Seo, Sang Lyun Nam, Joon Mo Lee, Sung Eun Namkoong, Chong Kook Kim, Yong Wan Kim, Woong Shick Ahn; Cancer Res Treat. 2003;35(3):181-190. Published online June 30, 2003; DOI: https://doi.org/10.4143/crt.2003.35.3.181

Abstract PDF

PURPOSE
Despite the significance of the p53 adenoviral vector in cancer gene therapy, an advanced strategy for the development of preferential tumor cell-specific delivery and the long-term persistent gene expression control of p53 are required. In this study, the time-course expression patterns of p53 and E6, on cervical cancer cells, were investigated to obtain a molecular level understanding of the cell-dependent tumor growth suppression effects of a recombinant adenovirus expressing p53, both in vitro and in vivo. MATERIALS AND METHODS: The expressions of p53 and E6 in CaSki, SiHa, HeLa, HeLaS3, C33A and HT3 cervical cancer cell lines were examined. After infection with AdCMVp53, the cell growth inhibition was studied via cell count, MTT and Neutral red assays. After transfecting the AdCMVp53 and AdCMVLacZ into the cancer cells-xenografted nude mice, the anti-tumor effects were investigated for one month. RESULTS: The p53 protein levels were more notably expressed in the CaSki and HeLa than in the SiHa and HeLaS3 On day 6, the p53 was only detected in the HeLaS3. In contrast, the p53 expression was highly maintained in the C33A and HT3. The E6 mRNA levels gradually decreased in only the CaSki and HeLa. The growth suppression effects also showed cell-dependent patterns, which were consistent with the reciprocal expression patterns of p53 and E6. After transfection of the AdCMVp53, into the CaSki- and SiHa-xenografted nude mice, the tumor size was remarkably decreased in the SiHa cells as compared to that in the AdCMVLacZ transfected mice, indicating cell-specific growth inhibition patterns.
CONCLUSION
The adenovirus-mediated p53 gene transfection was very effective both in vitro and in vivo. Also, the anti-tumor effects were accomplished via the differential role of p53-specific apoptotic cell death, which was dependent on the cervical cancer cell line.

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Immunization with Adenoviral Vectors Carrying Recombinant IL-12 and E7 Enhanced the Antitumor Immunity against Human Papillomavirus 16-associated Tumor
Eun-Kyung Park, Young-Wook Kim, Joon-Mo Lee, Sung-Eun NamKoong, Do-Gang Kim, Heung-Jae Chun, Byoung-Don Han, Su-Mi Bae, Hyun-Sun Jin, Jeong-Im Sin, Woong-Shick Ahn
Cancer Research and Treatment.2005; 37(1): 63. CrossRef

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E-Cadherin Expression and p53 Alterations in Soft Tissue Sarcomas: A Possible Role in Epithelial Differentiation: Jin Young Yoo, Seok Jin Kang, Woong Shick Ahn, Byung Kee Kim; Cancer Res Treat. 2001;33(4):343-349. Published online August 31, 2001; DOI: https://doi.org/10.4143/crt.2001.33.4.343

Abstract PDF

PURPOSE
We investigated the expressions of E- Cadherin and p53 in soft tissue tumors to determine their significance in sarcoma development and/or progression and to assess their potential correlation with epithelial features.
MATERIALS AND METHODS
A total of 79 soft tissue sarcomas, including 10 tumors comprising epithelial components, were studied immunohistochemically in paraffin-embedded tissue sections. Further analysis was performed on 61 tumors by the application of a polymerase chain reaction technique and a direct sequence analysis procedure applied to exons 5 through 8 in the p53 gene.
RESULTS
E-Cadherin was expressed at the cell-cell boundaries in 8 (10%) tumors: 5 of grade 2 and 3 of grade 3. Of these, six (being 60% of the total of 10 tumors containing epithelial elements) contained and two did not contain histologic evidence of epithelial differentiation. Overexpression of p53 was detected in 26 (33%) samples, 7 of which demonstrated mutations in the p53 gene. No association was established between E-Cadherin immunoreactivities and p53 abnormalities. Tumor grade was found to be strongly correlated with p53 alterations (p=0.01) but not with E-Cadherin expression (p=0.09).
CONCLUSION
These data confirm a role for altered p53 in the pathogenesis of soft tissue sarcomas and suggest a possible role for E-Cadherin in the maintenance of epithelial architecture in these tumors regardless of p53 status.

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Cancer Research and Treatment.2004; 36(2): 146. CrossRef

3,793 View
18 Download
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