KCA
Search
- Page Path
- HOME > Search
Original Articles
- Immunization with Adenoviral Vectors Carrying Recombinant IL-12 and E7 Enhanced the Antitumor Immunity against Human Papillomavirus 16-associated Tumor
- Eun-Kyung Park, Young-Wook Kim, Joon-Mo Lee, Sung-Eun NamKoong, Do-Gang Kim, Heung-Jae Chun, Byoung-Don Han, Su-Mi Bae, Hyun-Sun Jin, Jeong-Im Sin, Woong-Shick Ahn
- Cancer Res Treat. 2005;37(1):63-70. Published online February 28, 2005
- DOI: https://doi.org/10.4143/crt.2005.37.1.63
-
Abstract
PDF
PubReader
ePub Purpose Human papillomavirus (HPV) infection has a significant role in cervical carcinogenesis, and HPV oncoprotein E7 plays an important part in the formation and maintenance of cervical cancer. Interleukin-12 (IL-12) has been reported to induce a cellular immune response, and to suppress the tumor growth and the E7 production. Here we describe the use of adenoviral delivery of the HPV 16 E7 subunit (AdE7) along with adenoviral delivery of IL-12 (AdIL-12) in mice with HPV-associated tumors.
Materials and Methods Mice were injected with TC-1 cells to establish TC-1 tumor, and then they were immunized with AdIL-12 and/or AdE7 intratumorally. The anti tumor effects induced by AdIL-12 and/or E7 were evaluated by measuring the size of the tumor. E7-specific antibody and INF-γ production in sera, and the T-helper cell proliferative responses were then measured. Cytotoxic T-lymphocyte (CTL) and T cell subset depletion studies were also performed.
Results Combined AdIL-12 and AdE7 infection at the tumor sites significantly enhanced the antitumor effects more than that of AdIL-12 or AdE7 single infection. This combined infection resulted in regression of the 9 mm sized tumors in 80% of animals as compare to the PBS group. E7-specific antibody and INF-γ production in the sera, and the T-helper cell proliferative responses were significantly higher with coinfection of AdIL-12 and AdE7 than with AdIL-12 or AdE7 alone. CTL response induced by AdIL-12 and AdE7 in the coinjected group suggested that tumor suppression was mediated by mostly CD8+ and only a little by the CD4+ T cells.
Conclusion IL-12 and E7 application using adenovirus vector showed antitumor immunity effects against TC-1 tumor, and this system could be use in clinical applications for HPV-associated cancer. (ED note: nice abstract.)
-
Citations
Citations to this article as recorded by
- Development of a replication‐deficient adenoviral vector‐based vaccine candidate for the interception of HPV16‐ and HPV18‐induced infections and disease
Selina Khan, Koen Oosterhuis, Kerstin Wunderlich, Evelien M. Bunnik, Melissa Bhaggoe, Satish Boedhoe, Santusha Karia, Renske D.M. Steenbergen, Leontien Bosch, Jan Serroyen, Sarah Janssen, Hanneke Schuitemaker, Jort Vellinga, Gert Scheper, Roland Zahn, Jer
International Journal of Cancer.2017; 141(2): 393. CrossRef - Immune responses and protective efficacy of a recombinant swinepox virus expressing HA1 against swine H1N1 influenza virus in mice and pigs
Jiarong Xu, Dongyan Huang, shichao Liu, Huixing Lin, Haodan Zhu, Bao Liu, Chengping Lu
Vaccine.2012; 30(20): 3119. CrossRef - Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs
Jiarong Xu, Dongyan Huang, Shichao Liu, Huixing Lin, Haodan Zhu, Bao Liu, Chengping Lu
Virus Research.2012; 167(2): 188. CrossRef
- Development of a replication‐deficient adenoviral vector‐based vaccine candidate for the interception of HPV16‐ and HPV18‐induced infections and disease
- 8,887 View
- 37 Download
- 3 Crossref
- Photodynamic Effects of Radachlorin® on Cervical Cancer Cells
- Su-Mi Bae, Yong-Wook Kim, Joon-Mo Lee, Sung-Eun Namkoong, Sei-Jun Han, Jong-Ki Kim, Chang-Hee Lee, Heung-Jae Chun, Hyun-Sun Jin, Woong-Shick Ahn
- Cancer Res Treat. 2004;36(6):389-394. Published online December 31, 2004
- DOI: https://doi.org/10.4143/crt.2004.36.6.389
-
Abstract
PDFPubReaderePub
Purpose Photodynamic therapy (PDT) is a novel treatment modality, which produces local tissue necrosis with laser light following the prior administration of a photosensitizing agent. Radachlorin® has recently been shown to be a promising PDT sensitizer. In order to elucidate the antitumor effects of PDT using Radachlorin® on cervical cancer, growth inhibition studies on a HPV-associated tumor cell line, TC-1 cells
in vitro and animals with an established TC-1 tumorin vivo were determined.Materials and Methods TC-1 tumor cells were exposed to various concentrations of Radachlorin® and PDT, with irradiation of 12.5 or 25 J/cm2 at an irradiance of 20 mW/cm2 using a Won-PDT D662 laser at 662 nm
in vitro . C57BL/6 mice with TC-1 tumor were injected with Radachlorin® via different routes and treated with PDTin vivo . A growth suppression study was then used to evaluate the effects at various time points after PDT.Results The results showed that irradiation of TC-1 tumor cells in the presence of Radachlorin® induced significant cell growth inhibition. Animals with established TC-1 tumors exhibited significantly smaller tumor sizes over time when treated with Radachlorin® and irradiation.
Conclusion PDT after the application of Radachlorin® appears to be effective against TC-1 tumors both
in vitro andin vivo .-
Citations
Citations to this article as recorded by- Photodynamic Therapy for Thyroid Cancer
Julia Inglot, Joanna Katarzyna Strzelczyk, Dorota Bartusik-Aebisher, David Aebisher
BioMed.2025; 5(1): 8. CrossRef - Photodynamic therapy: Innovative approaches for antibacterial and anticancer treatments
Marketa Kolarikova, Barbora Hosikova, Hanna Dilenko, Katerina Barton‐Tomankova, Lucie Valkova, Robert Bajgar, Lukas Malina, Hana Kolarova
Medicinal Research Reviews.2023; 43(4): 717. CrossRef - Applications of Photodynamic Therapy in Endometrial Diseases
Gabriela Correia-Barros, Beatriz Serambeque, Maria João Carvalho, Carlos Miguel Marto, Marta Pineiro, Teresa M. V. D. Pinho e Melo, Maria Filomena Botelho, Mafalda Laranjo
Bioengineering.2022; 9(5): 226. CrossRef - Photodynamic treatment with purpurin 18 effectively inhibits triple negative breast cancer by inducing cell apoptosis
Pengyun Huang, Baoting Zhang, Qiuju Yuan, Xie Zhang, Wingnang Leung, Chuanshan Xu
Lasers in Medical Science.2021; 36(2): 339. CrossRef - Assessing of integration of ionizing radiation with Radachlorin-PDT on MCF-7 breast cancer cell treatment
R. Ghoodarzi, V. Changizi, A. R. Montazerabadi, N. Eyvazzadaeh
Lasers in Medical Science.2016; 31(2): 213. CrossRef - Chlorophyll a Covalently Bonded to Organo-Modified Translucent Silica Xerogels: Optimizing Fluorescence and Maximum Loading
M. García-Sánchez, I. Serratos, R. Sosa, T. Tapia-Esquivel, F. González-García, F. Rojas-González, S. Tello-Solís, A. Palacios-Enriquez, J. Esparza Schulz, A. Arrieta
Molecules.2016; 21(7): 961. CrossRef - Evaluation of Silicon Phthalocyanine 4 Photodynamic Therapy against Human Cervical Cancer Cells <i>in Vitro</i> and in Mice
Jill A. Gadzinski, Jianxia Guo, Brian J. Philips, Per Basse, Ethan K. Craig, Lisa Bailey, Joseph Latoche, John T. Comerci, Julie L. Eiseman
Advances in Biological Chemistry.2016; 06(06): 193. CrossRef - Combination of Photodynamic Therapy and Specific Immunotherapy Efficiently Eradicates Established Tumors
Jan Willem Kleinovink, Pieter B. van Driel, Thomas J. Snoeks, Natasa Prokopi, Marieke F. Fransen, Luis J. Cruz, Laura Mezzanotte, Alan Chan, Clemens W. Löwik, Ferry Ossendorp
Clinical Cancer Research.2016; 22(6): 1459. CrossRef - Development of radiolabeled radachlorin complex as a possible tumor targeting agent
Yousef Fazaeli, Amir R. Jalilian, Fatemeh Rezaee, Tahereh Firouzyar, Sedigheh Moradkhani, Azar Bagheri, Abbas Majdabadi
Journal of Radioanalytical and Nuclear Chemistry.2015; 303(3): 1695. CrossRef - Photodynamic therapy: An adjunct to conventional root canal disinfection strategies
Shipra Singh, Rajni Nagpal, Naveen Manuja, Sashi Prabha Tyagi
Australian Endodontic Journal.2015; 41(2): 54. CrossRef - The efficacy of Radachlorin-mediated photodynamic therapy in human hepatocellular carcinoma cells
Hamidreza Mirzaei, Gholamreza Esmaeeli Djavid, Mahnaz Hadizadeh, Maryam Jahanshiri-Moghadam, Parastoo Hajian
Journal of Photochemistry and Photobiology B: Biology.2015; 142: 86. CrossRef - Chlorin e6 Derivative Radachlorin Mainly Accumulates in Mitochondria, Lysosome and Endoplasmic Reticulum and Shows High Affinity toward Tumors in Nude Mice in Photodynamic Therapy
Raktim Biswas, Jeong Hwan Moon, Jin‐Chul Ahn
Photochemistry and Photobiology.2014; 90(5): 1108. CrossRef - Microscopic analysis of the localization of two chlorin‐based photosensitizers in OSC19 tumors in the mouse oral cavity
Floor van Leeuwen–van Zaane, Pieter B.A.A. van Driel, Ute A. Gamm, Thomas J.A. Snoeks, Henriëtte S. de Bruijn, Angelique van der Ploeg–van den Heuvel, Clemens W.G.M. Löwik, Henricus J.C.M. Sterenborg, Arjen Amelink, Dominic J. Robinson
Lasers in Surgery and Medicine.2014; 46(3): 224. CrossRef - Photodynamic effect of Radachlorin on nerve and glial cells
M.A. Neginskaya, E.V. Berezhnaya, M.V. Rudkovskii, S.V. Demyanenko, A.B. Uzdensky
Photodiagnosis and Photodynamic Therapy.2014; 11(3): 357. CrossRef - Fluorescence optimization of chlorophyll covalently bonded to mesoporous silica synthesized by the sol–gel method
I.N. Serratos, F. Rojas-González, R. Sosa-Fonseca, J.M. Esparza-Schulz, V. Campos-Peña, S.R. Tello-Solís, M.A. García-Sánchez
Journal of Photochemistry and Photobiology A: Chemistry.2013; 272: 28. CrossRef - Efficacy and Safety of Photodynamic Therapy for Recurrent, High Grade Nonmuscle Invasive Bladder Cancer Refractory or Intolerant to Bacille Calmette-Guérin Immunotherapy
Joo Yong Lee, Richilda Red Diaz, Kang Su Cho, Meng Shi Lim, Jae Seung Chung, Won Tae Kim, Won Sik Ham, Young Deuk Choi
Journal of Urology.2013; 190(4): 1192. CrossRef - Crossed and Linked Histories of Tetrapyrrolic Macrocycles and Their Use for Engineering Pores within Sol-Gel Matrices
Miguel García-Sánchez, Fernando Rojas-González, E. Menchaca-Campos, Salvador Tello-Solís, R. Quiroz-Segoviano, Luis Diaz-Alejo, Eduardo Salas-Bañales, Antonio Campero
Molecules.2013; 18(1): 588. CrossRef - Photodynamic Therapy for Recurrent Head and Neck Malignancy
Yong-Sik Lee, Young-Chang Lim, Yeo-Jin Lee, Mun-Su Park, Jae-Myeong Kim
Korean Journal of Otorhinolaryngology-Head and Neck Surgery.2011; 54(4): 271. CrossRef - Evaluation of the effect of photoactivated disinfection with Radachlorin® against Streptococcus mutans (an in vitro study)
Reza Fekrazad, Majid Bargrizan, Sepideh Sajadi, Soodabeh Sajadi
Photodiagnosis and Photodynamic Therapy.2011; 8(3): 249. CrossRef - In vitro evaluation of Radachlorin® sensitizer for photodynamic therapy
Samuel Douillard, Isabelle Lhommeau, David Olivier, Thierry Patrice
Journal of Photochemistry and Photobiology B: Biology.2010; 98(2): 128. CrossRef - In vitro and in vivo evaluation of Radachlorin® sensitizer for photodynamic therapy
Samuel Douillard, David Olivier, Thierry Patrice
Photochemical & Photobiological Sciences.2009; 8(3): 405. CrossRef - Role of Microarray in Cancer Diagnosis
Hoguen Kim
Cancer Research and Treatment.2004; 36(1): 1. CrossRef
- Photodynamic Therapy for Thyroid Cancer
- 11,918 View
- 69 Download
- 22 Crossref
First
Prev
