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Original Articles
- Time-course Transcriptional Profiling of Human Amniotic Fluid-derived Stem Cells Using Microarray
- Yong Wook Kim, Hyun-Jung Kim, Su-Mi Bae, Young Jae Kim, Jong-Chul Shin, Heung-Jae Chun, Jong-Won Rhie, Jiyoung Kim, Haekwon Kim, Woong Shick Ahn
- Cancer Res Treat. 2010;42(2):82-94. Published online June 30, 2010
- DOI: https://doi.org/10.4143/crt.2010.42.2.82
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Abstract
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ePub Purpose To maintain the homeostasis of stem cells and prevent their ability to initiate tumorigenesis, it is important to identify and modify factors that prevent or accelerate stem cell senescence. We used microarrays to attempt to identify such factors in human amniotic fluid (HAF)-derived stem cells.
Materials and Methods To identify gene expression changes over a time course, we compared gene expression profiles of HAF-derived stem cells in different passages (1st, 2nd, 4th, 6th, 8th, and 10th) using a Sentrix Human illumina microarray.
Results Of the 25,804 genes in the microarray chip, 1,970 showed an over 2-fold change relative to the control (the 1st passage)-either upregulated or downregulated. Quantitative real-time PCR validated the microarray data for selected genes: markedly increased genes were CXCL12, cadherin 6 (CDH6), and folate receptor 3 (FOLR3). Downregulated genes included cyclin D2, keratin 8, insulin-like growth factor 2 (IGF2), natriuretic peptide precursor B (NPPB) and cellular retinoic acid binding protein 2 (CRABP2). The expression pattern of the selected genes was consistent with the microarray data except for CXCL12 and IGF2. Interestingly, the expression of NPPB was dramatically downregulated along the time course; it was almost completely shut-down by the 10th passage. In contrast, FOLR3 mRNA expression was dramatically increased.
Conclusion Taken together, although a function for NPPB and FOLR3 in stem cell senescence has not been reported, our results strongly suggest that NPPB and/or FOLR3 play a significant role in the regulation of stem cell senescence.
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Citations
Citations to this article as recorded by
- Recent Advances in Folates and Autoantibodies against Folate Receptors in Early Pregnancy and Miscarriage
Xue-Yun Qin, Si-Yao Ha, Lu Chen, Tao Zhang, Ming-Qing Li
Nutrients.2023; 15(23): 4882. CrossRef - Cancer Stem Cells: Emergent Nature of Tumor Emergency
Yaroslav R. Efremov, Anastasia S. Proskurina, Ekaterina A. Potter, Evgenia V. Dolgova, Oksana V. Efremova, Oleg S. Taranov, Aleksandr A. Ostanin, Elena R. Chernykh, Nikolay A. Kolchanov, Sergey S. Bogachev
Frontiers in Genetics.2018;[Epub] CrossRef - Systematic identification of an integrative network module during senescence from time-series gene expression
Chihyun Park, So Jeong Yun, Sung Jin Ryu, Soyoung Lee, Young-Sam Lee, Youngmi Yoon, Sang Chul Park
BMC Systems Biology.2017;[Epub] CrossRef - Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections
Lucas-Sebastian Spitzhorn, Md Shaifur Rahman, Laura Schwindt, Huyen-Tran Ho, Wasco Wruck, Martina Bohndorf, Silke Wehrmeyer, Audrey Ncube, Ines Beyer, Carsten Hagenbeck, Percy Balan, Tanja Fehm, James Adjaye
Stem Cells International.2017; 2017: 1. CrossRef - Scavenger receptor class A member 5 ( SCARA5 ) and suprabasin ( SBSN ) are hub genes of coexpression network modules associated with peripheral vein graft patency
Richard D. Kenagy, Mete Civelek, Shinsuke Kikuchi, Lihua Chen, Anthony Grieff, Michael Sobel, Aldons J. Lusis, Alexander W. Clowes
Journal of Vascular Surgery.2016; 64(1): 202. CrossRef - Amniotic Fluid-Derived Stem Cells (AFSC) and Their Application in Cell Therapy and Tissue Engineering
Syeda Zahra Anum, Seyed Raheel Muzavir, Ahmad Hassan, Amir Ali Khan, Aftab Ahmad
Razavi International Journal of Medicine.2015;[Epub] CrossRef - Simvastatin Modulates Mesenchymal Stromal Cell Proliferation and Gene Expression
Dalila Lucíola Zanette, Julio Cesar Cetrulo Lorenzi, Rodrigo Alexandre Panepucci, Patricia Vianna Bonini Palma, Daiane Fernanda dos Santos, Karen Lima Prata, Wilson Araújo Silva, Benedetta Bussolati
PLOS ONE.2015; 10(4): e0120137. CrossRef - Senescence and longevity in amniotic fluid derived cells
Z. Chen, A. Jadhav, F. Wang, M. Perle, R. Basch, B. K. Young
Stem Cell Discovery.2013; 03(01): 47. CrossRef - Amniotic Fluid and Amniotic Membrane Stem Cells: Marker Discovery
Maria G. Roubelakis, Ourania Trohatou, Nicholas P. Anagnou
Stem Cells International.2012; 2012: 1. CrossRef - Applications of Amniotic Membrane and Fluid in Stem Cell Biology and Regenerative Medicine
Kerry Rennie, Andrée Gruslin, Markus Hengstschläger, Duanqing Pei, Jinglei Cai, Toshio Nikaido, Mahmud Bani-Yaghoub
Stem Cells International.2012; 2012: 1. CrossRef
- Recent Advances in Folates and Autoantibodies against Folate Receptors in Early Pregnancy and Miscarriage
- 12,826 View
- 63 Download
- 10 Crossref
- Immunization with Adenoviral Vectors Carrying Recombinant IL-12 and E7 Enhanced the Antitumor Immunity against Human Papillomavirus 16-associated Tumor
- Eun-Kyung Park, Young-Wook Kim, Joon-Mo Lee, Sung-Eun NamKoong, Do-Gang Kim, Heung-Jae Chun, Byoung-Don Han, Su-Mi Bae, Hyun-Sun Jin, Jeong-Im Sin, Woong-Shick Ahn
- Cancer Res Treat. 2005;37(1):63-70. Published online February 28, 2005
- DOI: https://doi.org/10.4143/crt.2005.37.1.63
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Abstract
PDFPubReaderePub
Purpose Human papillomavirus (HPV) infection has a significant role in cervical carcinogenesis, and HPV oncoprotein E7 plays an important part in the formation and maintenance of cervical cancer. Interleukin-12 (IL-12) has been reported to induce a cellular immune response, and to suppress the tumor growth and the E7 production. Here we describe the use of adenoviral delivery of the HPV 16 E7 subunit (AdE7) along with adenoviral delivery of IL-12 (AdIL-12) in mice with HPV-associated tumors.
Materials and Methods Mice were injected with TC-1 cells to establish TC-1 tumor, and then they were immunized with AdIL-12 and/or AdE7 intratumorally. The anti tumor effects induced by AdIL-12 and/or E7 were evaluated by measuring the size of the tumor. E7-specific antibody and INF-γ production in sera, and the T-helper cell proliferative responses were then measured. Cytotoxic T-lymphocyte (CTL) and T cell subset depletion studies were also performed.
Results Combined AdIL-12 and AdE7 infection at the tumor sites significantly enhanced the antitumor effects more than that of AdIL-12 or AdE7 single infection. This combined infection resulted in regression of the 9 mm sized tumors in 80% of animals as compare to the PBS group. E7-specific antibody and INF-γ production in the sera, and the T-helper cell proliferative responses were significantly higher with coinfection of AdIL-12 and AdE7 than with AdIL-12 or AdE7 alone. CTL response induced by AdIL-12 and AdE7 in the coinjected group suggested that tumor suppression was mediated by mostly CD8+ and only a little by the CD4+ T cells.
Conclusion IL-12 and E7 application using adenovirus vector showed antitumor immunity effects against TC-1 tumor, and this system could be use in clinical applications for HPV-associated cancer. (ED note: nice abstract.)
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Citations
Citations to this article as recorded by- Development of a replication‐deficient adenoviral vector‐based vaccine candidate for the interception of HPV16‐ and HPV18‐induced infections and disease
Selina Khan, Koen Oosterhuis, Kerstin Wunderlich, Evelien M. Bunnik, Melissa Bhaggoe, Satish Boedhoe, Santusha Karia, Renske D.M. Steenbergen, Leontien Bosch, Jan Serroyen, Sarah Janssen, Hanneke Schuitemaker, Jort Vellinga, Gert Scheper, Roland Zahn, Jer
International Journal of Cancer.2017; 141(2): 393. CrossRef - Immune responses and protective efficacy of a recombinant swinepox virus expressing HA1 against swine H1N1 influenza virus in mice and pigs
Jiarong Xu, Dongyan Huang, shichao Liu, Huixing Lin, Haodan Zhu, Bao Liu, Chengping Lu
Vaccine.2012; 30(20): 3119. CrossRef - Immune responses and protection efficacy of a recombinant swinepox virus expressing HA1 against swine H3N2 influenza virus in mice and pigs
Jiarong Xu, Dongyan Huang, Shichao Liu, Huixing Lin, Haodan Zhu, Bao Liu, Chengping Lu
Virus Research.2012; 167(2): 188. CrossRef
- Development of a replication‐deficient adenoviral vector‐based vaccine candidate for the interception of HPV16‐ and HPV18‐induced infections and disease
- 8,885 View
- 37 Download
- 3 Crossref
- Photodynamic Effects of Radachlorin® on Cervical Cancer Cells
- Su-Mi Bae, Yong-Wook Kim, Joon-Mo Lee, Sung-Eun Namkoong, Sei-Jun Han, Jong-Ki Kim, Chang-Hee Lee, Heung-Jae Chun, Hyun-Sun Jin, Woong-Shick Ahn
- Cancer Res Treat. 2004;36(6):389-394. Published online December 31, 2004
- DOI: https://doi.org/10.4143/crt.2004.36.6.389
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Abstract
PDFPubReaderePub
Purpose Photodynamic therapy (PDT) is a novel treatment modality, which produces local tissue necrosis with laser light following the prior administration of a photosensitizing agent. Radachlorin® has recently been shown to be a promising PDT sensitizer. In order to elucidate the antitumor effects of PDT using Radachlorin® on cervical cancer, growth inhibition studies on a HPV-associated tumor cell line, TC-1 cells
in vitro and animals with an established TC-1 tumorin vivo were determined.Materials and Methods TC-1 tumor cells were exposed to various concentrations of Radachlorin® and PDT, with irradiation of 12.5 or 25 J/cm2 at an irradiance of 20 mW/cm2 using a Won-PDT D662 laser at 662 nm
in vitro . C57BL/6 mice with TC-1 tumor were injected with Radachlorin® via different routes and treated with PDTin vivo . A growth suppression study was then used to evaluate the effects at various time points after PDT.Results The results showed that irradiation of TC-1 tumor cells in the presence of Radachlorin® induced significant cell growth inhibition. Animals with established TC-1 tumors exhibited significantly smaller tumor sizes over time when treated with Radachlorin® and irradiation.
Conclusion PDT after the application of Radachlorin® appears to be effective against TC-1 tumors both
in vitro andin vivo .-
Citations
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R. Ghoodarzi, V. Changizi, A. R. Montazerabadi, N. Eyvazzadaeh
Lasers in Medical Science.2016; 31(2): 213. CrossRef - Chlorophyll a Covalently Bonded to Organo-Modified Translucent Silica Xerogels: Optimizing Fluorescence and Maximum Loading
M. García-Sánchez, I. Serratos, R. Sosa, T. Tapia-Esquivel, F. González-García, F. Rojas-González, S. Tello-Solís, A. Palacios-Enriquez, J. Esparza Schulz, A. Arrieta
Molecules.2016; 21(7): 961. CrossRef - Evaluation of Silicon Phthalocyanine 4 Photodynamic Therapy against Human Cervical Cancer Cells <i>in Vitro</i> and in Mice
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Advances in Biological Chemistry.2016; 06(06): 193. CrossRef - Combination of Photodynamic Therapy and Specific Immunotherapy Efficiently Eradicates Established Tumors
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Clinical Cancer Research.2016; 22(6): 1459. CrossRef - Development of radiolabeled radachlorin complex as a possible tumor targeting agent
Yousef Fazaeli, Amir R. Jalilian, Fatemeh Rezaee, Tahereh Firouzyar, Sedigheh Moradkhani, Azar Bagheri, Abbas Majdabadi
Journal of Radioanalytical and Nuclear Chemistry.2015; 303(3): 1695. CrossRef - Photodynamic therapy: An adjunct to conventional root canal disinfection strategies
Shipra Singh, Rajni Nagpal, Naveen Manuja, Sashi Prabha Tyagi
Australian Endodontic Journal.2015; 41(2): 54. CrossRef - The efficacy of Radachlorin-mediated photodynamic therapy in human hepatocellular carcinoma cells
Hamidreza Mirzaei, Gholamreza Esmaeeli Djavid, Mahnaz Hadizadeh, Maryam Jahanshiri-Moghadam, Parastoo Hajian
Journal of Photochemistry and Photobiology B: Biology.2015; 142: 86. CrossRef - Chlorin e6 Derivative Radachlorin Mainly Accumulates in Mitochondria, Lysosome and Endoplasmic Reticulum and Shows High Affinity toward Tumors in Nude Mice in Photodynamic Therapy
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Photochemistry and Photobiology.2014; 90(5): 1108. CrossRef - Microscopic analysis of the localization of two chlorin‐based photosensitizers in OSC19 tumors in the mouse oral cavity
Floor van Leeuwen–van Zaane, Pieter B.A.A. van Driel, Ute A. Gamm, Thomas J.A. Snoeks, Henriëtte S. de Bruijn, Angelique van der Ploeg–van den Heuvel, Clemens W.G.M. Löwik, Henricus J.C.M. Sterenborg, Arjen Amelink, Dominic J. Robinson
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M.A. Neginskaya, E.V. Berezhnaya, M.V. Rudkovskii, S.V. Demyanenko, A.B. Uzdensky
Photodiagnosis and Photodynamic Therapy.2014; 11(3): 357. CrossRef - Fluorescence optimization of chlorophyll covalently bonded to mesoporous silica synthesized by the sol–gel method
I.N. Serratos, F. Rojas-González, R. Sosa-Fonseca, J.M. Esparza-Schulz, V. Campos-Peña, S.R. Tello-Solís, M.A. García-Sánchez
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Journal of Urology.2013; 190(4): 1192. CrossRef - Crossed and Linked Histories of Tetrapyrrolic Macrocycles and Their Use for Engineering Pores within Sol-Gel Matrices
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- Photodynamic Therapy for Thyroid Cancer
- 11,918 View
- 69 Download
- 22 Crossref
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