Skip Navigation
Skip to contents

Cancer Res Treat : Cancer Research and Treatment

OPEN ACCESS

Previous issues

Page Path
HOME > Browse articles > Previous issues
22 Previous issues
Filter
Filter
Article category
Keywords
Authors
Volume 29(2); April 1997
Prev issue Next issue
Original Articles
Effects of Combination Chemotherapy Depending on The Expression of Neuron Specific Enolase (NSE), P-Glycoprotein (PgP) and Glutathione S-Transferase (GST)-pai in Advanced Non-Small Cell Lung Cancer
Chul Won Jung, Jong Wook Shin, Sang Jae Lee, Eon Sub Park
J Korean Cancer Assoc. 1997;29(2):189-197.
AbstractAbstract PDF
PURPOSE
Neuroendocrine differentiation and expression of drug resistance may affect the response to chemotherapy and the prognosis of advanced non-small cell lung cancer. We conducted retrospective study to evaluate the possibilities of neuroendocrine differentiation and drug resistance markers being used as prognostic factors in patients with non-small cell lung cancer. MATERIAL AND METHOD: Immunohistochemical staining of NSE, PgP and GST-pai with polyclonal antibodies in pathologic specimens of 47 patients with non-small cell lung cancer were done. The relationship between the expression of the markers and the response to cisplatin-based chemotherapy and the overall survival were assessed.
RESULTS
NSE staining was positive in 17% and there was no difference between adenocarcinoma and squamous cell carcinoma. NSE positive patients showed increased response rate (63% vs 26%, p=0.049) and prolonged response duration (15.8mo vs 4.5mo, p=0.0007). But there was no difference in overall survival between NSE positive and negative groups. The PgP positive rate was 17% and GST-pai positive rate was 47%. No correlations were found among the expression of drug resistance, the sensitivity to chemotherapy and overall survival.
CONCLUSION
Patients with non-small cell lung cancer with positive NSE showed increased response rate to chemotherapy and prolonged response duration but overall survival was not related to NSE expression. Expression of PgP and GST- were not important in predicting the prognosis in non-small cell lung cancer.
  • 2,378 View
  • 18 Download
Close layer
Prognostic Significance of the Extent of Lymph Node Dissection in Gastric Cancer
Han Kwang Yang, Sung Bum Kang, Kuhn Uk Lee, Kuk Jin Choe, Yong Il Kim, Jin Pok Kim
J Korean Cancer Assoc. 1997;29(2):198-203.
AbstractAbstract PDF
PURPOSE
The purpose of this retrospective study is to evaluate the prognostic significance of lymph node dissection in gastric cancer surgery.
MATERIALS AND METHODS
We analysed the survival curves of 1661 cases of gastric cancer patients who received gastric resection from 1987 to 1991, in whom the number of dissected lymph nodes is more than 9 (to minimize stage migration). We used modified TNM system with numeric classification.
RESULT
The average number of resected lymph nodes of the study group was 31.5 and there was no significant difference in the average number of resected lymph nodes during the study period. In stage I, IIa, IIb and IV, there was no significant difference in survival curves according to the number of resected lymph nodes (25, 35 or 45). However, in stage IIIa, the 5-year survival rate (5YSR) was better in the patients (n=171) of more than 25 lymph nodes than in the patiens (n=104) of less than 26 resected lymph nodes (64.1% vs. 59.9%, p=0.031). In stage IIIb, 5YSR was better in the patients (n=206) of more than 35 lymph nodes than in the patients (n=307) of less than 36 lymph nodes (33.2% vs. 18.3%, p=0.029). In multivariate analysis, number of dissected lymph node was an independent prognostic factor.
CONCLUSION
Survival benefit of extended lymph node dissection in the gastric cancer surgery is limited in the stage IIIa and IIIb and the recommended number of dissection is more than 35. This study also suggests a positive correlation between tumor stage and required quantity of lymph node dissection for better survival (>25 for stage IIIa and >35 for stage IIIb).
  • 2,361 View
  • 14 Download
Close layer
Midkine Gene Expression in Gastric Cancer Tissues
Hyun Joo Kwak, Yong Bae Kim, Byung Soh Min, Ho Young Maeng, Sung Hoon Song, Hye Weon Chung, Tae Soo Kim, Hei Cheol Chung, Sun Young Rha, Hyun Cheol Chung, Sung Hoon Noh, Joo Hang Kim, Jae Kyung Roh, Jin Sik Min, Byung Soo Kim
J Korean Cancer Assoc. 1997;29(2):204-211.
AbstractAbstract PDF
PURPOSE
We evaluated the clinical significance of the tumor growth factor, midkine (MK), in paired gastric cancer and normal tissues.
MATERIALS AND METHODS
Twenty paired normal and cancer tissues were tested for MK mRNA expression by Northern blot analysis. Vessel staining was done by immunohistochemical staining using CD-31 monoclonal antibody (Dako).
RESULTS
MK mRNA was mainly expressed in cancer tissues (11 versus 1). Lymph node metastasis, pathological stage and tumor differentiation did not correlate with MK expression. However, MK expression rate increased with increment in tumor size (p=0.05). Microvascular density did not correlate with tumor invasion, lymph node metastasis, and pathological stages. However, there was a tendency of vascular density increment with MK expression in T1-T2 stage.
CONCLUSION
MK was mainly expressed in larger gastric cancer tissues suggesting its role in cancer growth in vivo. But no definite correlation between MK expression and tumor microvascular density was found.
  • 3,296 View
  • 22 Download
Close layer
Detection of p53 Gene Mutations in Gastric Cancers: Comparative study of Single Strand Conformational polymorphism migration Technique (SSCP) and Non-Isotopic RNase Cleavage Assay (NIRCA)
Young Jin Kim, Ji Yun Kook, Ji Hee Lee, Hyeong Rok Kim, Jae Hwan Joo, Dong Yi Kim, Shin Kon Kim, Soon Pal Suh, Jin Pok Kim
J Korean Cancer Assoc. 1997;29(2):212-219.
AbstractAbstract PDF
PURPOSE
The aim of the present study was: (a) to determine the frequency of p53 mutations by single strand conformational polymorphism analysis of polymerase chain reaction products (PCR-SSCP), Non-Isotopic RNase Cleavage Assay (NIRCA) and immunohistochemical staining with monoclonal antibody; and (b) to compare the correlations among these methods.
MATERIALS AND METHODS
Abberations of the p53 gene in 24 primary gastric carcinomas were examined by PCR-SSCP, NIRCA and immunohistochemical staining. Of these surgically resected gastric adenocarcinomas, 23 were advanced gastric carcinomas and 1 was early gastric cancer. Using PCR-SSCP and NIRCA, the presence of mutations in exons 4-9 was evaluated. Using the mouse specific anti-human p53 monoclonal antibody, we also looked for overexpression of the p53 protein in tissue sections.
RESULTS
In 5 cases shifted bands were reproducibly identified by PCR-SSCP, and two mutations were identified in exon 4 and three in 5 & 6. The mutations of exon 4 were detected by NIRCA in 5 cases, exon 5 & 6 in 6 cases, and exon 7 in 2 cases. The p53 mutations detected by PCR-SSCP were also detected by NIRCA except one case. Thirteen of the tumor samples were positively stained with the monoclonal antibody for p53 protein. There was no correlation between p53 mutations detected by NIRCA and expression of p53 protein by immunohistochemical staining.
CONCLUSIONS
Our results in this group of patients suggest that NIRCA is more sensitive than PCR-SSCP in detecting p53 mutations, and expression of p53 protein by immunohistochemical staining does not directely represent the genetic changes of p53 gene.
  • 2,260 View
  • 12 Download
Close layer
Studies on the Mechanism of Hypoxic Increase of VEGF Expression in the Hep3B Human Hepatoma Cells
Yoo Wook Kwon, Soo Kyung Bae, Jung Ae Kim, Kyu Won Kim, Byung Chae Park
J Korean Cancer Assoc. 1997;29(2):220-226.
AbstractAbstract PDF
PURPOSE
Hepatocellular carcinoma (HCC), a typical hypervasculized tumor is very sensitive to hypoxia and vascular endothelial growth factor (VEGF) has previously been identified to be up-regulated in response to hypoxia in several cell types. However, the molecular mechanisms by which hypoxia is sensed by the cells remain enigmatic. To investigate whether calcium and AP-1 are involved in hypoxia-sensing mechanism, we performed following experiments.
MATERIALS AND METHODS
Hep3B cells were grown in hypoxic condition. To assess cell viability, MTT assay was performed. To investigate the effect of calcium and AP-1, northern blot analysis was performed after treatment with BAPTA/AM.
RESULTS
The expression of VEGF was significantly up-regulated by hypoxia in Hep3B, hepatocellular carcinoma cell line. The increased expression of VEGF induced by hypoxia was blocked by the addition of BAPTA/AM, a cytosolic calcium chelator to the media. In addition, we found that the expression of c-jun protooncogene was also up-regulated by hypoxia. Hypoxic increase of c-jun expression was also normalized by the treatment with BAPTA/AM.
CONCLUSION
These results suggest that the increased expression of VEGF by hypoxia is mediated through the calcium and c-jun signalling pathway in the Hep3B human hepatoma cell lines.
  • 2,279 View
  • 15 Download
Close layer
Hepatic Intraarterial Chemotherapy in Unresectable Hepatic Metastases of Colorectal Cancer
Jin Cheon Kim, Han Il Lee, Chang Sik Yu, Hee Won Chung, Sang Wee Kim, Jeong Sin Lee, Kun Choon Park
J Korean Cancer Assoc. 1997;29(2):227-234.
AbstractAbstract PDF
PURPOSE
Unresectable hepatic metastases of colorectal cancer does not seem to be amenable to the various treatment modalities. We modified hepatic intraarterial chemotherapy by different installation of port and regimen.
MATERIALS AND METHODS
Between July 1989 to December 1995, 27 patients of colorectal cancer with unresectable liver metastases were randomly allocated into either hepatic intraarterial (HA, 11 patients) or systemic intravenous (IV, 16 patients) chemotherapy after primary tumor resection. Chemo-port was installed with preservation of hepatic arterial flow. One cycle of HA regimen included 5-fluorouracil (5-FU) and mitomycin-C (MMC) with or without leucovorin (LV) for 14 days every month. The IV regimen included 5-FU and LV for 5 days every month. Both HA and IV chemotherapy were continued from 6 to 12 cycles.
RESULTS
The response exceeding partial remission was experienced in six patients (55%) among 11 patients in the HA group, while only two (13%) patients showed response among sixteen patients in the IV group. One year survival was not different between two groups. Although lethal toxicity was not found, patients showed marked increase of the performance scale (ECOG) in both groups.
CONCLUSION
Although survival benefit was not prominent, higher response rate with tolerable complication was found in the HA group. Prudent selection of effective drugs and combination of systemic chemotherapy are needed to improve the survival with minimal complication.
  • 2,663 View
  • 14 Download
Close layer
Efficiency of Positron Emission Tomography (PET) in Diagnosis of Breast Cancer
Seok Jin Nam, Jee Hyung Noh, Byung Tae Kim
J Korean Cancer Assoc. 1997;29(2):235-242.
AbstractAbstract PDF
PURPOSE
PET developed on the basis of biochemical characteristics of malignant tissues where the increase in glucose metabolism. Therefore, early and accurate detection of primary or metastatic lesion can be expected. This study is to compare PET and other traditional methods in detection of primary breast carcinoma and metastatic lesion, and to find the advantage of PET.
MATERIALS AND METHODS
We compared and analysed the results of PET, US, mammogr aphy, bone scan, CT and biopsy reports of the 46 patients who were examined in our hospital from September 1, 19094 to July 31, 1995. PET Trace 200 cyclotron and PET Advanced Scenner were used for FDG synthesis and imaging respectively.
RESULTS
10 of the 12 cases who had preoperative PET were consistent with the results of mammography and ultrasound and 9 of the 10 cases were consistent with the pathologic results. In one case, which did mammography and PET after excis ional biopsy, PET gaves a false positive result. In the detection of axilliary node metastasis, PeT after excisional biopsy, PET gaves a false positive result. In the detection of axilliary node metastasis, PET shows 100% sensitivity and specificity but bone scan shows 100%, 72.2% respectively.
CONCLUSION
PET is useful for diagnosis of primary breast cancer and is superior to bone scan in specificity for diagnosis of bone metastasis and in early detection of response to treatment. Because PET cannot exclude false (+) in inflamm atory lesion, continuous investigation is needed for establishment of indication and reduction of false (+), false (-).In spite of high cost, PET may become a new and useful diagnostic tool.
  • 2,474 View
  • 16 Download
Close layer
Expression of DNA Topoisomerase II and P-Glycoprotein in Breast Cancer
Hye Rim Park, Eun Ha Jung, Jin Hee Sohn, Young Euy Park
J Korean Cancer Assoc. 1997;29(2):243-249.
AbstractAbstract PDF
PURPOSE
To determine whether the expression of DNA topoisomerase II and P-glycoprotein are of prognostic value. MATERIALS AND METHODS: We evaluated the expression of DNA topoisomerase II and P-glycoprotein immunohistochemically in a retrospective study of samples from 44 patients with breast cancer. Thirty two among 44 patients (72.7%) received chemotherapeutic treatments (CMF or FAC protocol) and/or tamoxifen postoperatively.
RESULTS
P-glycoprotein was detected in the 27 samples of 44 patients (61.3%). The expression of P-glycoprotein was increased in the patients older than 50 years, with distant metastases, and with death on follow-up. DNA topoisomerase II was detected in the 34 samples of 44 patients (77.2%). The expression of topoisomerase II was increased in the patients younger than 50 years, with recurrent tumor, with distant metastases, and with death on follow-up. The expression of P-glycoprotein and topoisomerase II was not correlated with other clinico-pathological factors including the size of primary tumor, involvement of lymph node, histologic grade, and clinical stage. The correlation between expression of P-glycoprotein and topoisomerase II was not significant.
CONCLUSION
The immunohistochemical evaluation of P-glycoprotein and topoisomerase II before treatment in breast cancer has little clinical prognostic value.
  • 2,269 View
  • 12 Download
Close layer
Palliative Radiotherapy for Painful Bone Metastases
Won Dong Kim, Woo Yoon Park
J Korean Cancer Assoc. 1997;29(2):250-256.
AbstractAbstract PDF
No abstract available
  • 2,122 View
  • 12 Download
Close layer
Quantitative Correlation between the Biochemical Markers and The Extent of Metastatic Bone Tumors
Soo Kyung Kim, Deok Su Cho, Hyun Seon Baek, Se Hwa Kim, Min Chul Kim, Sung Hye Shin, Young Hee Lee, Joo Hung Park
J Korean Cancer Assoc. 1997;29(2):257-265.
AbstractAbstract PDF
PURPOSE
We investigated the usefulness of urinary pyridinoline (uPyr) and deoxypyridinoline (uDpyr) and serum osteocalcin as markers of bone metastasis, particularly focussing on quantitative correlation between the degree of bone metastasis and the level of biochemical markers.
MATERIALS AND METHODS
By using ELISA method we measured the levels of uPyr, uDpyr, and osteocalcin in 100 cancer patients of whom 58 patients had bone metastasis, 42 had no bone metastasis, and 44 control subjects.
RESULTS
There was a significant difference in uPyr level between the patients with bone metastasis and the patients without bone metastasis or control group (mean+/-SD, 70.26+/-43.11 vs 38.93+/-21.48 or 25.13+/-8.81 nM/mM Creatinine, p<0.05). And uDpyr level showed more significant elevation in the patients with bone metastasis than in the patients without bone metastasis and in control group (12.63+/-7.51 vs 6.44+/-3.58 and 4.23+/-1.70 nM/mM Creatinine p<0.05). Osteocalcin level showed no significant difference among groups. We could demonstrate a significant quantitative correlation between the extent of bone metastasis and the amount of uPyr (r=0.7482, p<0.001) or uDpyr (r=0.5992, p<0.001).
CONCLUSION
uPyr and uDpyr were significantly increased in metastatic bone tumors and quantitatively correlated well with the extent of bone metastasis. Therefore we can use these two markers as an evidence of bone metastasis. Further studies are recommended to decide the usefulness of these markers in the early detection of bone metastasis and in the assessment of response to antiresorptive treatments.
  • 2,073 View
  • 12 Download
Close layer
Prognostic Significance of Histologic Features, DNA Content, Expression of Proliferating Cell Nuclear Antigen (PCNA), c-fos Protein and Transforming Growth Factor (TGF)-alpha and -beta in Giant Cell Tumor of Bone
Hee Kyung Chang, Sung Hun Yoon, Jae Do Kim, Man Ha Huh
J Korean Cancer Assoc. 1997;29(2):266-279.
AbstractAbstract PDF
PURPOSE
This study was attempted to investigate the prevalence of the expression of c-fos protein, TGF-alpha and -beta, PCNA , DNA ploidy pattern and histopathological parameters of giant cell tumor (GCT) of bone and to correlate with prognosis and to extend our understanding on tumorigenesis of GCT.
MATERIALS AND METHODS
Twenty eight cases of paraffin-embedded tissue were studied, classified as recurrent (5 cases) and non-recurrent group (12cases) within the limits of the cases which afforded surgical material on first operation.
RESULTS
No significant difference was observed in cellularity of stromal cells, atypia of stromal and giant cells, presence of hemorrhage and necrosis between recurrent and non-recurrent group. However, presence of more than 10 mitotic figures in 10 high power fields in recurrent group was significantly higher than non-recurrent group (p<0.05). The immunoreactivity for PCNA was seen only in nuclei of stromal cells, whereas nuclei of giant cells showed negative staining. The positivity of PCNA revealed no significant difference between non-recurrent (mean; 40.9%) and recurrent group (34.4%). The expression of c-fos oncogene was seen in 5 cases (100%) in recurrent group, and 8 cases (66.7%) in non-recurrent group, and no significant difference was seen. No significant difference of expression of TGF-alpha was seen in 5 cases (100%) in recurrent group and in 11 cases (91.7%) in non-recurrent group. The expression of TGF-beta in stromal cells was significantly higher in non-recurrent group (80%) compared to recurrent group (100%) (p<0.05). In DNA analysis out of 18 cases, 4 cases (22.2%) were aneuploidy and 14 cases (77.8%) were diploidy. Among 4 aneuploidy cases, 3 cases (75%) had no recurrence, and 1 case (25%) had metastasis to lung and expired. No significant difference of DNA ploidy pattern was seen between the recurrent and non-recurrent group.
CONCLUSION
Presence of more than 10 mitotic figures in 10 high power fields and less expression of TGF-beta are related to higher possibility of recurrence and it is suggested that the number of mitotic figure (more than 10/10HPF) and expression of TGF-beta could be helpful parameters in predicting recurrence of GCT.
  • 2,325 View
  • 14 Download
Close layer
A Study on the Loss of Heterozygosity of the p53 Gene in Primary Uterine Cervical Carcinomas
Jin Woo Kim, Chun Geun Lee, Yeo Won Sohn, Hong Ki Min, Su Mi Han, Eun Young Cho, Kyung Sook Kim, Joon Mo Lee, Sung Eun Namkoong
J Korean Cancer Assoc. 1997;29(2):280-290.
AbstractAbstract PDF
PURPOSE
Allelic deletion of p53 tumor suppressor gene have been observed frequently in a variety of human tumors. These losses are believed to contribute to the development of human cancers. But the loss of heterozygosity (LOH) data on chromosome 17p are rare and controversial with respect to cervical carcinomas. So, we tried to elucidate the frequency of p53 locus LOH in primary cervical carcinoma and compared the LOH data with clinicopathological parameters.
MATERIALS AND METHODS
In order to detect LOH within one of the well-known tumor suppressor gene, p53, three intragenic polymorphisms (exon 1, exon 4, and intron 6) and one microsatellite distal to the p53 gene (D17S5) were examined. Paired DNA samples from 55 primary uterine cervical carcinomas and normal bloods were studied for the chromosomal allelic loss of p53 gene locus by polymerase chain reaction (PCR), the presence of human papilloma virus (HPV), and the presence of p53 gene point mutation by PCR-single conformation polymorphism (SSCP) analysis. And the relationships between allelic losses of this gene and conventional clinicopathological parameters were evaluated.
RESULTS
We could increase the heterozygosity of the p53 gene up to 1 (100%). The observed allelic loss rate of the p53 locus in informative cases was 5.5% (3/55) and the observed allelic loss rate of the D17S5 locus in informative cases was 8.7% (4/46) . Only one of the four patients with LOH at the D17S5 locus showed a concomittant allelic loss of the p53 gene. The overall LOH incidence of the chromosomal region comprising 17p13.1 (p53) to 17p13.3 (D13S5) was 10.9% (6/55). All the samples contained at least one of the oncogenic HPV type 16 and/or 18 sequences. No shifted bands were observed in the PCR-SSCP analysis of the p53 gene. The LOH of the p53 gene was not related to other parameters including clinical stage, histological type, and degree of differentiation.
CONCLUSION
Concerning with the results above, we conclude that the allelic imbalance of the p53 gene itself is not implicated as a major contributing factor in the malignant transformation or the tumor progression in HPV-positive cervical cancers. Another putative tumor suppressor gene which has more important function than p53 gene in cervical carcinogenesis might exist between these two loci [p53 (17p13.1) and D17S5 (17p13.3)].
  • 2,569 View
  • 13 Download
Close layer
Germline Mutation of Rb1 Gene in Korean Retinoblastoma Patients
Ja Lok Ku, Young Suk Yu, Jae Gahb Park
J Korean Cancer Assoc. 1997;29(2):291-298.
AbstractAbstract PDF
PURPOSE
Retinoblastoma is an intraocular tumor occurring almost exclusively in young children. Germline mutations in the Rb1 gene confer hereditary predisposition to retinoblastoma. To identify germline mutations in the Rb1 gene in Korean retinoblastoma patients, we analyzed germline mutations of the Rb1 gene in 4 Korean retinoblastoma patients from 3 families.
MATERIALS AND METHODS
All patients were bilaterally affected in early childhood. First patient and second patient were same family members (SNU-RB1-1 and -2), and in the third patient (SNU-RB2), tumor cells had metastasized to the central nervous system 2 years after treatment of retinoblastoma. Fourth patient (SNU-RB3) developed secondary osteosarcoma in the nasal cavity 15 years after treatment of retinoblastoma. We have used PCR-SSCP analysis and DNA sequencing analysis to screen germline mutations.
RESULTS
We found one missense mutation in the fourth patient (SNU-RB3). This was a point mutation from AAA (lysine) to GAA (glutamine) at codon 616 in exon 19 of the Rb1 gene.
CONCLUSION
We confirmed one germline mutation of the Rb1 gene in one Korean patient who had a sporadic bilateral retinoblastoma and osteosarcoma. Identification of the germline mutation in Rb1 gene would help to improve the presymptomatic diagnosis and clinical management of retinoblastoma patients.
  • 2,389 View
  • 18 Download
Close layer
Cytogenetic Effects of Radiotherapy on Peripheral Blood Lymphocytes of Cancer Patients
Hyung Sik Lee, Young Min Choi, Won Joo Hur, Kyung Hee Kim, Sang Dong Sin, Jin Yeong Han, Jung Man Kim, Hyo Jin Kim
J Korean Cancer Assoc. 1997;29(2):299-308.
AbstractAbstract PDF
PURPOSE
To evaluate the frequency and types of chromosome aberrations induced by ionizing radiation in cancer patients before and after radiotherapy.
MATERIALS AND METHODS
Thirty cases with various types of cancers such as rectum, lung, uterine cervix, lymphoma, and etc. were studied. The time of analyses was within 1 month after radiotherapy. The mean total radiation dose was 54 Gy. RESULTS: The incidence of aberrant metaphases prior to radiation treatment was 2.2% and increased significantly after treatment to 29.7% (p<0.001). Also the number of chromosomal aberrations per cell was increased by a factor of 38.5. A comparison of chromosome abnormalities observed before and after treatment indicated that dicentrics, acentrics, derivatives and translocations were increased by a factor of 155.5, 99, 55.5 and 23.8, respectively, after radiation treatment. Breakages were located at all chromosomes except Y chromosome and total number of identifiable breakages was 426 in 900 observed lymphocyte metaphases. Distribution of break points revealed the increase of number of break points in chromosome 1,2,5 and 11q.
CONCLUSION
Ionizing radiation produces more asymmetrical than symmetrical chromosome aberrations and more two-break than one-break anomalies in radiation-treated cancer patients.
  • 3,001 View
  • 20 Download
Close layer
Role of Cell Surface Mucin on Invasion and Metastasis of HM7 Colon Cancer Cells
Wan Hee Yoon, Hae Duck Park, Kyu Lim, Byung Doo Hwang
J Korean Cancer Assoc. 1997;29(2):309-320.
AbstractAbstract PDF
PURPOSE
Mucinous colorectal cancers have a poorer prognosis than which colorectal cancer produce low amount of mucin, but the exact mechanism is not well understood. The present study was undertaken to elucidate the exact mechanism of invasion and metastasis of high mucin producing colon cancer cells using mucin glycosylation inhibitor, benzyl-alpha-N-acetylgalactosamine.
MATERIALS AND METHODS
To evaluate the effect of glycosylated mucin on invasion and metastasis, in vitro invasion, metalloproteinases (MMPs) activity, cell-matrix protein binding, cell-cell aggregation, as well as endothelial leukocyte adhesion molecule (ELAM-1) binding and cell surface expression of various mucin related antigens were analyzed.
RESULTS
MMPs activity in conditioned medium and invasion of ECM-coated porous filters by benzyl-alpha-GalNAc treated HM7 cells were decreased. There was no difference between control and treated HM7 cells in terms of matrix protein binding assay, but treated HM7 cells showed higher homotypic cell adhesion. The binding activity of treated HM7 cells to ELAM-1 was significantly decreased and fixed cell binding of MoAb SNH-3, 19-9 (specific for sialyl-Lewis X and sialyl-Lewis A) were also significantly decreased.
CONCLUSION
These results suggest that glycosylated mucin modulates ELAM-1 binding, MMPs activity and homotypic cell adhesion, therefore enhance invasive and metastatic properties of human colon cancer cells.
  • 2,280 View
  • 16 Download
Close layer
Combined Therapy of Gene Therapy using the Herpes Simplex Virus Thymidine Kinase, and Retinoic Acid
Jae Yong Park, Steven M Albelda
J Korean Cancer Assoc. 1997;29(2):321-331.
AbstractAbstract PDF
PURPOSE
Metabolic cooperation via gap junctional intercellular communication (GJIC) is an important mechanism of the bystander effect in gene therapy using the Herpes Simplex Virus thymidine kinase/ganciclovir (HSVtk/GCV) "prodrug" system. Since retinoids have been reported to increase GJIC by induction of connexin expression, we hypothesized that these compound could be used to augment the HSVtk/GCV bystander effect.
MATERIALS AND METHODS
We transferred HSVtk gene to AB12 cell line that express connexin43 as a component of gap junction. We examined the effects of retinoic acid (RA) on GJIC utilizing a functional double-dye transfer study. To evaluate the bystander effect in vivo, a murine subcutaneous tumor model was established. Before proceeding with comparisons of HSVtk/GCV mediated bystander cell killing, we evaluated the effects of RA on flank tumor growth in order to rule out a potential antitumor effect of RA alone. Then we determined the effects of retinoic acid on bystander-mediated cell killing in an animal model.
RESULTS
Addition of all-trans retinoic acid increased GJIC in AB12 cell line and was associated with more efficient GCV induced bystander killing in animal model. HSVtk transduced tumors in mice treated with the combination of GCV and retinoids were significantly smaller than those treated with GCV or retinoids alone.
CONCLUSION
These results provide evidence that retinoids can augment the efficiency of cell killing with the HSVtk/GCV system by enhancing bystander effects and may thus be a promising new approach to improve response in gene therapy utilizing the HSVtk/GCV system to treat tumors.
  • 2,254 View
  • 14 Download
Close layer
Comparison of Tropisetron with Ondansetron in the Prevention of Cisplatin-induced Nausea and Vomiting
Kyung Shick Lee, Ji Youn Han, Hanlim Moon, Bok Kun Lee, Seok Goo Cho, Jong Youl Jin, Young Sun Hong, Hoon Kyo Kim
J Korean Cancer Assoc. 1997;29(2):332-339.
AbstractAbstract PDF
PURPOSE
Tropisetron (Nabovan (R)) is a new specific 5-HT3 receptor antagonist with a long terminal half life in plasma and high bioavailability after oral intake. We compared the antiemetic effectiveness and tolerability of tropisetron with ondansetron in the highly emetogenic chemotherapy (including cisplatin > or =50 mg/m2).
MATERIALS AND METHODS
Thirty-nine patients were administered in a randomized, multicenter, open, cross-over study and received either tropisetron plus dexamethasone (n=31) or ondansetron plus dexamethasone (n=34) during six days of two successive cycles of chemotherapy.
RESULTS
Total control of vomiting with either Ondansetron or tropisetron was 94.2 % vs 93.5 % in D1 (P=0.157); 90.6 % vs 93.1 % in D2 (P=0.18); 90.3 % vs 93.1 % in D3 (P=0.655); 96.4% vs 96.4 % in D4 (P=0.157); 96.4 % vs 100 % in D5 (P=0.317); 96.4 % vs 100% in D6, respectively. The duration of nausea showed significant decreasements in tropisetron at D5 and D6 (P=0.025, P=0.03, respectively), but the severity of nausea and performance status showed no significance. Headache and constipation were the most common side effects in both groups.
CONCLUSION
There was no significant difference in efficacy and tolerability between tropisetron and ondansetron in the cisplatin-based chemotherapy.
  • 3,637 View
  • 31 Download
Close layer
Malignant Gastric Tumors Excluding Adenocarcinoma
Byung Jo Bae, Ki Ho Park, Soo Sang Sohn, Sun Kyo Song, Wansik Yu
J Korean Cancer Assoc. 1997;29(2):340-343.
AbstractAbstract PDF
No abstract available
  • 1,956 View
  • 12 Download
Close layer
Avascular Necrosis of Bone Complicating Treatment of Malignant Lymphoma: Report of 6 cases
Hyung Chan Suh, Jee Sook Hahn, Bum Seok Kim, Seok Lee, So Young Chong, Yoo Hong Min, Yun Woong Ko
J Korean Cancer Assoc. 1997;29(2):344-351.
AbstractAbstract PDF
BACKGROUND
Several reports have documented the association of avascular necrosis (AVN) of bone and the treatment of malignant lymphoma with steroid-containing chemotherapy. It is important to recognize these conditions, as they can be mistaken for those of lymphomatous involvement.Cases: This report describes the experience at the Severance hospital over a 10-year period with 6 patients in whom AVN developed during or following treatment of malignant lymphoma. Four patients of non-Hodgkin's lymphoma and two of Hodgkin's disease were treated with steroid-containing chemotherapy. The predominant symptom is pain on motion or weight bearing. Symptoms leading to diagnosis of AVN developed between 5 and 27 months after starting prednisolone (mean 17.8 months), and the mean cumulative dose of prednisolone to the onset of AVN was 4,447 mg (range, 1,800~9,490 mg). All but one were involved in both hip joint. Diagnosis was based on the radiologic appearance, and in the majority radiographic changes consistent with AVN were present at the time of presentation of symptoms. Four patients received total hip replacement and two had conservative care.
CONCLUSION
Patients with malignant lymphoma who developed pain on joint during or after the use of steroid-containing chemothearpy should be carefully investigated with MRI and radionuclear bone scan for early diagnosis and proper management.
  • 2,534 View
  • 16 Download
Close layer
A Case of Primary Splenic Angiosarcoma Associated with Kasabach-Merritt Syndrome
Jin Seok Jeon, Gyu Taek Lee, Ki Ju Han, Jae Ho Byun, Seung Kyu Park, Jong Ho Won, Seung Ho Baick, Dae Sik Hong, Hee Sook Park, Hae Kyung Lee, So Young Jin
J Korean Cancer Assoc. 1997;29(2):352-357.
AbstractAbstract PDF
PURPOSE
Primary malignant vascular neoplasms of the spleen are rare. It has been known that the prognosis was very poor and the splenectomy before rupture could increase survival. No effective chemotherapeutic protocol for angiosarcomas has yet to be established but patients with or without metastatic disease may be treated by chemotherapy. MATERIAL AND METHODS: We experienced a case of primary splenic angiosarcoma in a 42-year-old woman with multiple purpuric skin rashes associated with consumptive coagulopathy:the Kasabach-Merritt syndrome. The CT showed spleen is diffusely enlarged and inhomogenously enhanced with multiple metastasis in the liver. The splenectomy was done and angiosarcoma was diagnosed. We treated her with conventional combination chemotherapy and obtained partial response. For additional response, high-dose chemotherapy and stem cell rescue with autologous peripheral blood stem cell transplantation was done.
RESULT
Afer splenectomy, platelet count return to normal. The follow up abdominal CT scan after treatment showed complete disappeared multiple metastatic lesions in the both lobe of liver and the patient has continued to do well four months following discharge.
CONCLUSION
We herein report our experience of a splenic angiosarcoma whose multiple hepatic metastases responded well to the high-dose chemotherapy.
  • 3,554 View
  • 18 Download
Close layer
Chronic Subdural Hematoma Associated with Dural Metastasis of Gastric Carcinoma
Ramiro Colleoni, Laercio Gomes Lourenco, Alexandre R Elias, Marcelo Itsatugo, Joao Norberto Stavale, Jose Carlos Del Grande, Chibly Michel Haddad, Fernando Menezes Braga
J Korean Cancer Assoc. 1997;29(2):358-361.
AbstractAbstract PDF
No abstract available
  • 2,354 View
  • 13 Download
Close layer
A Case of Malignant Mesothelioma of the Uterus
Ki Young Ryu, Joong Sik Shin, Sam Hyun Cho, Seung Ryong Kim, Jung Bae Yoo, Kyung Tai Kim, Youn Yeoung Hwang, Seok Hoon Jeon, Jung Dal Lee, Min Chul Lee
J Korean Cancer Assoc. 1997;29(2):362-362.
AbstractAbstract PDF
Malignant mesothelioma of the serosal surface of the uterus body is very rare and almost invariably malignant. Their diagnosis is made postoperatively in most instances.Various treatments have been described for malignant mesothelioma but effective method is still not known. A case of malignant mesothelioma of the uterus body with post-surgical pelvic persistence is presented. The diagnosis of carcinomatous mesothelioma was made postoperatively by pathologic examination and immunohistochemical reaction of the tumor cells.
  • 3,321 View
  • 52 Download
Close layer

Cancer Res Treat : Cancer Research and Treatment
Close layer
TOP