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The p16INK4A Expression in Stomach Cancer , Colon Cancer and Hepatoma Cell Lines
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Sun Ju Choi, Soo Kie Kim, Se Jong Kim, Choon Myung Koh, Yoon Sun Park
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J Korean Cancer Assoc. 1998;30(3):527-535.
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 Abstract
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- PURPOSE
The p16(INK4A) gene encodes a specific inhibitor of cell cycle progression. In recent years, genetic deletion and altered expression of p16(INK4A) gene were frequently showed in many human cancers. So, the p16(INK4A) gene is considered as tumor suppressor gene. However, there has been a few data for the p16(INK4A) in gastric cancer, colon cancer, and hepatoma.So.we investigated the genetic deldtion and altered expression of p16(INK4A) in gastric cancer, colon cancer and hepatoma cell lines.
MATERIALS AND METHODS
The homozygous deletion of p16(INK4A) was examined by using PCR and the protein expression of p16(INK4A) by using Western blotting in cancer cell lines established from Korean patients: stomach cancer, colon cancer and hepatoma cell lines.
RESULTS
Homozygous deletion of p16(INK4A) was detected only 1 stomach cancer cell line out of 13 cell lines examined.
The p16(INK4A) was detected in 3 of 13 cancer cell line.
These results showed the low frequency of p16(INK4A) homozygous deletion and high frequency of p16(INK4A) expression alteration in stomach cancer, colon cancer and hepatoma cell lines.
CONCLUSION
In this study, it may be suggested that the altered pl6(INK4A) expression as well as p16(INK4A) gene deletion play important role in oncogenesis. Further studies to determine the mechanism of p16(INK4A) gene inactivation are expected.
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In Vitro Growth Inhibition of Human Ovarian Cancer Cell Lines by Mitosene Analogues
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Dong Soo Cha, Soo Kie Kim, Chan Mug Ahn, Sun Ju Choi, Yoon Sun Park, Sang Won Han
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J Korean Cancer Assoc. 1997;29(3):437-444.
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Abstract
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- No abstract available
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In Vitro Chemosensitivaty Test of SK-302 on Human Gastric Carcinoma Cell Lines
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Soo Kie Kim, Woon Seob Shin, Yoon Sun Park, Sun Ju Choi, Kyung Ho Lee, Joo Young Park, Choon Myung Koh, Chan Mug Ahn, Weon Sub Park
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J Korean Cancer Assoc. 1995;27(5):703-711.
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Abstract
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- SK-30ZB with quinomycin-related structure is considered to be potent antitumor antibiotic. There were several reports that quinomycins and their derivatives showed wide spectrum of anti-tumor activity. But there were no reports on anti-gastric tumor activity. We fould SK- 302B with anti-gastric tumor activity in vitro using our established anti-tumorsubstance screening system. Recent attention has been focused on the possibility of predicting the effectiveness of anti-cancer drugs on individual tumor through chemosensitivity tests. However, optimal conditions for in vitro chemosensitivity test on human gastric carcinoma cell lines were not well established. We attempted to establish optimal conditions and to evaluate compara- tively in vitro tumor cell cytotoxicity between SK-302B and adriamycin, using 7 human gastric carcinoma celi lines. The optimal cell number and culture duration for in vitro tumor cell cytotoxicity(TCC) and colony formation inhibition assay(CFIA) is 5x10(3) - 1x10(4) cells/well (TCC) and 2.510(3) - 10(3) cells/well(CFIA), 4 days(TCC) and 10 - l4 days(CFIA), respectively, for all gestric cancer cell lines. Under these conditions, we performed chemosensitivity test. Measurement of cytotoxicity(IC50) and colony forming efficiency revealed higher tumoricidal activity of SK-302 B against all tested gastric cancer cell lines than compared to that of adriamycin. In conclusion, we preseneted optimal conditions for in vitro chemosensitivity test on human gastric carcinoma cell lines. Using optimal conditions of this sytem, it could be demonstrated that SK-302B exerted a potent gastric cancer cell growth inhibition in vitro. Therefor, These data suggest that SK-302B may have a possibility of development as promising candidate of anti-gastric cancer chemotherapeutic agent.
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KCA
