-
Low Doses of Nonylphenol Promote Growth of Colon Cancer Cells through Activation of ERK1/2 via G Protein‒Coupled Receptor 30
-
Ming Xie, Jin-Long Liang, Han-Dong Huang, Mai-Jian Wang, Tao Zhang, Xue-Feng Yang
-
Cancer Res Treat. 2019;51(4):1620-1631. Published online May 15, 2019
-
DOI: https://doi.org/10.4143/crt.2018.340
-
-
 Abstract
 PDF PubReader ePub
- Purpose
Nonylphenol (NP) is an endocrine disruptor found in products such as cleaners, plastics, and detergents. It exerts actions similar to endogenous 17β-estradiol (E2) and is reported to influence various cancers. However, its role in colon cancer remains elusive.
Materials and Methods
Colon cancer cell lines COLO 205 and SW480 were employed in our study. The cells were treated with NP or E2 followed by measurement of apoptosis and proliferation using flow cytometry and MTT assays, respectively. G protein–coupled estrogen receptor 30 (GPR30) expression was visualized using immunofluorescence and Western blot. To investigate the underlying mechanism, the expression levels of GPR30, p-protein kinase A (PKA), c-myc, cyclin D1, and ERK1/2 were analyzed using Western blot. Meanwhile, the GPR30 antagonist G15 was utilized to validate the role of GPR30 in colon cancer progression. Finally, the effect of a GPR30 inhibitor on tumor growth was determined in vivo using tumor xenograft mouse models.
Results
NP facilitated the proliferation of colon cancer cells and induced apoptosis failure in vitro. Western blot revealed increased GPR30 expression levels in response to NP treatment. Cyclin D1, p-PKA, c-myc, and proliferating cell nuclear antigen, proteins that regulate the cell cycle, were all upregulated by NP, and NP-mediated ERK1/2 activation and subsequent cell proliferation were abrogated by the GPR30 inhibitor G15. Moreover, colon cancer mice that received G15 administration demonstrated impaired tumor growth in vivo.
Conclusion
Low dose NP promotes the growth of colon tumors through GPR30-mediated activation of ERK1/2 signaling.
-
Citations
Citations to this article as recorded by  - Role of sex steroids in colorectal cancer: pathomechanisms and medical applications
Jianglan Wu
American Journal of Cancer Research.2024; 14(7): 3200. CrossRef - 17β-estradiol in colorectal cancer: friend or foe?
Zihong Wu, Chong Xiao, Jiamei Wang, Min Zhou, Fengming You, Xueke Li
Cell Communication and Signaling.2024;[Epub] CrossRef - Implications of estrogen and its receptors in colorectal carcinoma
Plabon Kumar Das, Joti Saha, Suja Pillai, Alfred K.‐Y. Lam, Vinod Gopalan, Farhadul Islam
Cancer Medicine.2023; 12(4): 4367. CrossRef - Endocrine-Disrupting Chemicals and Disease Endpoints
Changhwan Ahn, Eui-Bae Jeung
International Journal of Molecular Sciences.2023; 24(6): 5342. CrossRef - Hsp27, a potential EcR target, protects nonylphenol-induced cellular and organismal toxicity in Drosophila melanogaster
Shiwangi Dwivedi, Leonard Clinton D'Souza, Nidhi Ganesh Shetty, Shamprasad Varija Raghu, Anurag Sharma
Environmental Pollution.2022; 293: 118484. CrossRef - Effect of nonylphenol on the colonic mucosa in rats and intervention with zinc-selenium green tea (Camellia sinensis)
Shixu Li, Mucong Zheng, Xuefeng Yang, Jianling Zhang, Jie Xu, Jie Yu
Toxicology Research.2022; 11(1): 122. CrossRef - Nonylphenol regulates TL1A through the AhR/HDAC2/HNF4α pathway in endothelial cells to promote the angiogenesis of colorectal cancer
Tao Zhang, Wei-Wei Ning, Jie Zhang, Fu-Jian Xu, Xing-Qin Wang, Zheng-Biao Li, Ming Xie
Toxicology and Applied Pharmacology.2022; 436: 115854. CrossRef - Effects of subchronic exposure of nonylphenol on the expression of immune-related factors and estrogen receptors in the spleen of rats
Xiangjun Fu, Jie Xu, Chengyu Ni, Degang Yu, Haibo Wang, Pan Wang, Man Luo, Jie Yu
Environmental Sciences Europe.2022;[Epub] CrossRef - Mechanism of nonylphenol induced gastric inflammation through NF-κB/NLRP3 signaling pathway
Jie Xu, Shixu Li, Xuefeng Yang, Haibo Wang, Lina Ma, Yuan Shen, Jie Yu
Toxicology.2022; 479: 153294. CrossRef - Nonylphenol Promoted Epithelial–Mesenchymal Transition in Colorectal Cancer Cells by Upregulating the Expression of Regulator of Cell Cycle
Nian-jie Zhang, Yuanwei Zhang, Shuo Yin, Du-ji Ruan, Nian He, Xu Chen, Xue-feng Yang
Chemical Research in Toxicology.2022; 35(9): 1533. CrossRef - Co-exposure to BPA and DEHP enhances susceptibility of mammary tumors via up-regulating Esr1/HDAC6 pathway in female rats
Xuan Zhang, Cheng Cheng, Guopei Zhang, Mingyang Xiao, Liuli Li, Shengwen Wu, Xiaobo Lu
Ecotoxicology and Environmental Safety.2021; 221: 112453. CrossRef - Coiled-Coil Domain Containing 80 Suppresses Nonylphenol-Induced Colorectal Cancer Cell Proliferation by Inhibiting the Activation of ERK1/2
Jing Wang, Yuan-wei Zhang, Nian-jie Zhang, Shuo Yin, Du-ji Ruan, Nian He, Xu Chen, Xue-feng Yang
Frontiers in Cell and Developmental Biology.2021;[Epub] CrossRef - Residential proximity to industrial pollution sources and colorectal cancer risk: A multicase-control study (MCC-Spain)
Javier García-Pérez, Nerea Fernández de Larrea-Baz, Virginia Lope, Antonio J. Molina, Cristina O'Callaghan-Gordo, María Henar Alonso, Marta María Rodríguez-Suárez, Benito Mirón-Pozo, Juan Alguacil, Inés Gómez-Acebo, Nieves Ascunce, Mercedes Vanaclocha-Esp
Environment International.2020; 144: 106055. CrossRef
-
6,664
View
-
174
Download
-
15
Web of Science
-
13
Crossref
|
KCA
