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Somatic Mutations of APC Presenting Polymorphisms in the Hamartomatous Polyps of the Colon
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Jin Cheon Kim, Seon Ae Roh, Hee Cheol Kim, Chang Sik Yu, Nichoias E Beck, Walter F Bodmer
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J Korean Cancer Assoc. 1999;31(6):1288-1296.
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- No abstract available.
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Establishment and Biological Carcterization of Human Colorectal Carcinoma Cell Lines Expressiong Carcinoembryonic Antigen
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Jin Cheon Kim, In Chul Lee, Seon Ae Roh, Yoo Kyung Lee, Kyung Sin Kim, Kun Choon Park
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J Korean Cancer Assoc. 1996;28(1):63-72.
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Abstract
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- For serum carcinoembryonic antigen (CEA) is not always elevated in recurrence or metastasis of colorectal carcinoma (CRC), activation of colorectal carcinoma cells by CEA may be selective. We have established and characterized two CEA-expressing colorectal carcinoma cell lines which were analysed in relation to the clinical course. Two cell lines originated from rectal carcinoma were cultured in DME enhanced media and subcultured by 40 and 55 times respectively. AMC 4 grew in an anchrage-independent with delayed cell doubling time (93 hrs), while AMC 5 did in an anchorage-dependent with normal doubling time (43 hrs). Tumorigenesis revealed infiltrative moderate-differentiated adenocarcinoma in AMC 4 and expansile well-differentiated adenocarcinoma in AMC 5. Karyotyping by Trypsin-Giemsa banding showed 47, XY, 21(+ ), del (2q, 5q) and 46, XY, 5(+ ), 20(+ ), 12(- ) respectively. Both cell lines were aneuploid. AMC 5 expressed large amount of 55 kD NCA as well as 180 KD CEA, but AMC 4 did traces of them by indirect immunofluorescence and immunoblot. AMC 5 cells bound optimally to solid-phase type IV collegen, laminin, and CEA by 18.7¡¾ 0.4, 21.4 ¡¾ 0.6 and 17.7¡¾0.4, while AMC 4 cells bound less. The patient of AMC 4 showed liver metastasis on 6 months after curative surgery and AMC 5 did no evidence of recurrence. In conclusion, biological characteristics of two CEA-expressing CRC cell lines seemed to be consistent with their clinical course.
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The Effect of laminin and Type 4 Collagen on the Adhesion and Invasion of Human Gastrocytes and Gastric Cancer Cells In Vitro
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Byung Sik Kim, Jin Cheon Kim, Seon Ae Roh, Kun Choon Park, Kuk Jin Choe
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J Korean Cancer Assoc. 1995;27(3):360-374.
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Abstract
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- Extracellular matrix is important in the metastasis of tumor, and basement membrane acts not only as tissue barrier but also as adhesive substrate for tumor cell invasion. Laminin and type IV collagen are major components of basement membrane and important in tumor invasion. Adhesion and invasion studies about metastasis of the tumor have been performed mainly in colon cancer or breast cancer but rare in gastric cancer. The purpose of this study was to investigate the difference in the ability of adhesion to the basement membrane and their biological activity between the normal gastrocyte and gastric cancer cells. Then, The role of basement membrane in tumor invasion was assessed by the evaluation of the invasion of gastric cancer cells of different histological differentiation. Gastrocytes;, ere isolated from the surgically resected specimen by treating with collagenase and deoxyribonuclease. Normal gastrocytes were maintained viable up to 24 hours after isolation. The isolated cells were confirmed as gastric epithelial cells by indirect immuno- fluorescence staining for cytokeratin. Although the adhesion of normal gastrocyte was lower than that of gastric cancer cells, gastrocyte was bound to laminin and type IV collagen. The substrates were shown to play an important role for the differentiation and regeneration of gastrocytes also. In the invasion assay using transwell cell culture chamber coated with matrigel, laminin and type IV collagen enhanced tumor cell invasion (p<0.05). However, there was no difference according to histological differentiation. In conclusion, it can be suggested that laminin and type IV collagen must be important for the biologicaf activity of normal gastrocyte and metastasis of gastric cancer celL Type IV collagen enhances the invasion of gastric cancer cell more than laminin does.
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KCA
