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Lymphokine-activated killer(LAK) cell activity in tumor-transplanted mice(II)
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Sang Yun Nam, Yun Tai Lee, Young Il Kim, Si Young Kim, Kyung Sam Cho
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J Korean Cancer Assoc. 1992;24(3):365-377.
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 Abstract
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- No abstract available.
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Lymphokine-activated killer(LAK) cell activity in tumor-transplanted mice(I)
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Sang Yun Nam, Yun Tai Lee, Young Il Kim, Si Young Kim, Kyung Sam Cho
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J Korean Cancer Assoc. 1991;23(2):218-229.
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Abstract
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- No abstract available.
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Comparative Study on In Vitro Biological Activity of Human Natural and Recombinant Interleukin 2 ( IL 2 )
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Sang Yun Nam, Jai Kyung Park, Kyung Soo Hahm, Kyu Chul Choeh, Youn Mun Ha, Moon Hee Han, Yun Tai Lee, Yong Mook Choi
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J Korean Cancer Assoc. 1989;21(2):328-339.
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Abstract
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- Recently some authers af us have described successful production of human recombinant interleu- kin 2 (rlL 2). In concideration of future application in vitro and in vivo of rIL 2, this study was carried out in an attempt to confirm the biological activities of the rIL 2 and compare with those of human natural IL 2 (nlL 2) on a per unit basis. The two IL 2 preparations induced CTLL-2 cell proliferation at the equivalent level. This result showed that nIL 2 and rIL 2 activity could be evaluated with same assay system. Dose response of IL 2 in lymphokine-activated killer (LAK) cell induction was also similar. Greatest activity of LAK cells was induced with 500-1,000 U/ml (target, Raji) or 100 1,000 U/ml (target, K -562) of IL Z, and the activity decreases over 5,000 U/ml of rIL 2. In LAK cell induction kinetics, peak activity was seen after 4 (targe, Raji) to 5 day-culture (target, K-562) with rlL 2 and nIL 2. Thereafter, activity of LAK cells generated with nIL 2 declind whereas it was sustained (target, K-562) or enhanced successively (target, Raji) with rIL 2. Similar results were also seen in a prolonged activation of LAK cells for 9~14 days. Augmentation effect of IL2 on natural killer cell activity by 24 hr-treatment was comparable between nlL 2 and rlL 2 except that higher peak activity was observed with rIL 2 than with nIL 2. These data suggested that nIL 2 might be different from rIL 2 in some biological activities, although we cannot exclusively rule out the possibility that some other related lymphakines are present in nlL 2 preparation. Subsequent experiments for elucidation of the mechanisms for the differential activities demonstrated that the functional differences of the two IL 2 preparations observed above were due to neither lability nor poor IFN induction of nIL 2. Thus, it was assumed that activation or proliferation of any other cell populations than LAK effector or precursor cells, e.g., suppressor cells, might be involved in the mechanism.
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In Vitro Activation and expansion of Human Tumor - Infiltrating Lymphocytes ( TIL ) with Intreleukin 2 ( IL 2 ) and Their Antitumor - Cytotoxic Properties Against Autotogous Tumor Cells
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Kyu Chul Choeh, Sang Yun Nam, Jai Kyung Park, Youn Mun Ha, Yong Mook Choi, Chang Il Ahn
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J Korean Cancer Assoc. 1989;21(1):35-51.
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Abstract
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- To determine the effect of interleukin 2 (IL 2) on human tumor-infiltrating lymphocytes (TIL) from surgically excised solid tumors and to charscterize the antitwhor-cytotoxicity of activated TIL comparing with lymphokine-activated killer (LAK) cells generated from peripheral blood mononu- clear cells, this study wss performed. Single cell suspensions of TIL snd tumor cells were prepared from 20 surgical specimens (12 stomach canrers, 4 breast cancers, 1 lung cancer, 1 colon cancer, 1 ovarian cancer, and 1 cervix cancer) by mechanical and enzymatic means. And freshly isolated TIL were separated from tumor cells by fishing method. The amount of TIL obtained by the above means was 7.1x10+-6.7 (SD) cells/cm(3) of tumor mass with range of 0.8~27.5 x 10(5). The TIL prepatrations contaminated partially with tumor cells (10~20%) were cultured in IL 2 containing media. Cell numbers were reduced from the initial seeding density until about 7 days of culture. After 10 days of culture with IL 2, TIL increased in number with a concomitant disappearance of tumor cells, whereas there were slow decreases of lymphocytes and increases of tumor cells in control cultures. TIL were expanded between 0.5 and 118,000 fold increases for 10 to 34 days (2~9 subcultures). Partially purified natural IL 2-induced expansions of TIL were more rapid than highly purified recombinant IL 2-induced expansions, and showed 10 times high for 18 days of culture. The majority of TIL were T lymphocytes with range of 56~68.8% of Leu4+, 25.0~40. 8%of Leu2a+, and 0~51. 5% of Leu3a+cells. With continued in vitro expansion for 14-20 days, there was a concomitant increase in T lymphocytes (Leu4+) to 71~76.8% but the changes af phenotypes of Leu2a+ and Leu3a+ were variable. TIL were tested for cytotaxicity against fresh autologous and alfogenic tumor cells, and Raji and K562 targets in a 4-hr Cr release assay. IL 2-activated TIL showed more potent cytotoxicity against autologous tumor targets than allogenic (P<0.05). And cytotoxic activity of activated TIL in killing autologous tumor targets was higher level than that of LAK celis, but the defference was not statistically significant (0.05
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