Cancer Research and Treatment

Sang Uk Im

1 Article

Analysis of Cellular Changes Resulting from Forced Expression of Dickkopf-1 in Hepatocellular Carcinoma Cells: Mi Hee Kwack, Sun Young Hwang, In Seok Jang, Sang Uk Im, Jin Oh Kim, Moon Kyu Kim, Jung Chul Kim, Young Kwan Sung; Cancer Res Treat. 2007;39(1):30-36. Published online March 31, 2007; DOI: https://doi.org/10.4143/crt.2007.39.1.30

Purpose

Recent studies have shown that Dickkopf-1 (DKK-1) is overexpressed in some tumors, including hepatocellular carcinoma. However, the role of increased DKK-1 in these tumors is not known. In this study, the DKK-1 expression in hepatocellular carcinoma (HCC) cell lines was evaluated and the effect of DKK-1 overexpression in HCC cell lines was studied.

Materials and Methods

The expression of DKK-1 in hepatocellular carcinoma cell lines was evaluated by RT-PCR. Stable cell lines that overexpressed DKK-1 were established. Cell growth, adhesion, migration and invasion assays were performed.

Results

RT-PCR analysis showed that 5 out of 8 HCC cell lines expressed DKK-1. The forced expression of DKK-1 suppressed the growth of cells and increased the population of cells in the sub-G1 phase. In addition, DKK-1 reduced the cellular adhesion capacity to collagen type I and fibronectin, and it increased migratory capacity. However, overexpression of DKK-1 did not increase the invasion capacity of the HCC cell line.

Conclusion

Collectively, our data suggest that overexpression of DKK-1 affects the biology of HCC cells.

Citations

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