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Interleukin-2 ( IL-2 ) Production by Human Large Granular Lymphocyte ( LGL ) - Enriched Population
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K. H. Kim, S. Y. Nam, S. D. Lim
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J Korean Cancer Assoc. 1984;16(1):26-34.
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 Abstract
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- Human large granular lymphocytes(LGL) are known to be natural killer(NK) effector cells. NK cell activity is augmented by interferon(IFN) and interleukin-2(IL-2). Furthermore, it was reported that LGL produce IFN-a and IFN-r. We have performed this investigation to demonstrate whether LGL can produce IL-2 or not. LGL-enriched (Fraction I; Fr. I) and LGL-depleted (Fraction II , Fr.II) populations were prepared from human peripheral blood lymphocytes(PBL) on a discontinuous Percoll gradient and stimulated with phptohemagglutinin(PHA)-M for various time periods. The culture supernatants(CM) harvested were tested for IL-2 activity with cultured human PBL. After 48hrs, higher activities were seen in CM produced from Fr. II(Fr. II-CM)than those from Fr I (Fr I-CM). And maximal activity was detected in 72hr-cultured Fr. II-CRI. In contrast, within 24hrs, IL-2 activities in Fr. I-CM were higher than in Fr. II-CM. In these results, it was observed that LGL produce IL- 2 with a peak of productivity occurred early(about 24hr) compared with that occurred later(about 72hr) by T cells. And from the fact that LGL produce IFN and IL-2, we suppose both IFN and IL-2 can act as regul- atory molecules for NK cell activation. Addition of adherent cells(20% of effector cells) was shown to inhibit IL-2 production.
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Natural Killer ( NK ) Cell Activity Against Tumor Cell Line in the Presence of Alpha and Gamma - Interferon
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S. D. Lim, K. H. Kim, S. Y. Nam
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J Korean Cancer Assoc. 1983;15(1):1-14.
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Abstract
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- In 109 normal dornors and patients with cancer(stomach cancer; 60, hepatoma; 23, lung cancer: 13), natural killer(NK) cell activitiea were asaessed. Furthermore, a-and r-interfe- ron(IFN) was compared on their augmentation of NK cell activity and direct cytcstatic action. The findings in this study were as follows; 1) NK cell activities in patients with cancer were decreased when compared to the mean of normal donors. And the decreaaed NK cell activities were augmented to the normal level by IFN-treatment. 2) The patients with metastatic tumors exhibited a greater deficit than the patients wit#h local cancer in NK cell activity, but their NK cell activation with IFN was similar. 3) NK cell activities against stomach cancer cell line and hepatoma cell line were also .significantly enhanced by IFN-treatment. It was indicated that NK cell activation with IFN in patienta with cancer may show a favorable therapeutic effectiveness. 4) In NK cell activation, a-and r-IFN had comparable effect. 5) In direct cytostatic action, F-IFN was more effective than a-IFN, and r-IFN was suggested to be more active against tumor in clinical use. 6) The proportions of LGL(NK cell) in peripheral blood were not correlatcd with the NK cell activities determined by "Cr-release assay
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KCA
