Skip Navigation
Skip to contents

Cancer Res Treat : Cancer Research and Treatment

OPEN ACCESS

Author index

Page Path
HOME > Browse articles > Author index
Search
Kye-Hyun Nam 1 Article
Protein Expression Profile using Two-Dimensional Gel Analysis in Squamous Cervical Cancer Patients
Su-Mi Bae, Hyun-Jin Min, Guo Hua Ding, Sun-Young Kwak, Young-Lae Cho, Kye-Hyun Nam, Choong Hak Park, Yong-Wan Kim, Chong-Kook Kim, Byoung-Don Han, Young-Joo Lee, Do Kang Kim, Woong-Shick Ahn
Cancer Res Treat. 2006;38(2):99-107.   Published online April 30, 2006
DOI: https://doi.org/10.4143/crt.2006.38.2.99
AbstractAbstract PDFPubReaderePub
Purpose

Screening in cervical cancer is now progressing to discover candidate genes and proteins that may serve as biological markers and that play a role in tumor progression. We examined the protein expression patterns of the squamous cell carcinoma (SCC) tissues from Korean women with using two- dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight (MALDI- TOF) mass spectrometer.

Materials and Methods

Normal cervix and SCC tissues were solubilized and 2-DE was performed using pH 3~10 linear IPG strips of 17 cm length. The protein expression was evaluated using PDQuest 2-D software™. The differentially expressed protein spots were identified with a MALDI-TOF mass spectrometer, and the peptide mass spectra identifications were performed using the Mascot program and by searching the Swiss-prot or NCBInr databases.

Results

A total of 35 proteins were detected in SCC. 17 proteins were up-regulated and 18 proteins weredown-regulated. Among the proteins that were identified, 12 proteins (pigment epithelium derived factor, annexin A2 and A5, keratin 19 and 20, heat shock protein 27, smooth muscle protein 22 alpha, α-enolase, squamous cell carcinoma antigen 1 and 2, glutathione S-transferase and apolipoprotein a1) were protein previously known to be involved in tumor, and 21 proteins were newly identified in this study.

Conclusion

2-DE offers the total protein expression profiles of SCC tissues; further characterization of these differentially expressed proteins will give a chance to identify the badly needed tumor-specific diagnostic markers for SCC.

Citations

Citations to this article as recorded by  
  • The role of proteomics in the modern diagnosis of cervical cancer
    Aida U. Hamadyanova, Azalia S. Sultanmuratova, Aliya Kh. Disbiyanova, Svetlana N. Akhmadeyeva, Nikita O. Yadgarov, Liana E. Burangulova
    Journal of obstetrics and women's diseases.2022; 71(2): 113.     CrossRef
  • Understanding Cervical Cancer through Proteomics
    Fátima Martínez-Rodríguez, Jared E. Limones-González, Brenda Mendoza-Almanza, Edgar L. Esparza-Ibarra, Perla I. Gallegos-Flores, Jorge L. Ayala-Luján, Susana Godina-González, Eva Salinas, Gretel Mendoza-Almanza
    Cells.2021; 10(8): 1854.     CrossRef
  • Annexin A1 peptide and endothelial cell‐conditioned medium modulate cervical tumorigenesis
    Laila Toniol Cardin, Janesly Prates, Bianca Rodrigues da Cunha, Eloiza Helena Tajara, Sonia Maria Oliani, Flávia Cristina Rodrigues‐Lisoni
    FEBS Open Bio.2019; 9(4): 668.     CrossRef
  • Assessment of cellular and serum proteome from tongue squamous cell carcinoma patient lacking addictive proclivities for tobacco, betel nut, and alcohol: Case study
    Sapna Khowal, Samar H. Naqvi, Seema Monga, Swatantra K. Jain, Saima Wajid
    Journal of Cellular Biochemistry.2018; 119(7): 5186.     CrossRef
  • iTRAQ-based quantitative proteomic analysis of cervical cancer
    YIBING DING, MIN YANG, SHA SHE, HAIYAN MIN, XIAOMING XV, XIAOPING RAN, YONGZHENG WU, WEI WANG, LEI WANG, LONG YI, YIXUAN YANG, QIAN GAO
    International Journal of Oncology.2015; 46(4): 1748.     CrossRef
  • Differential expression analysis of Golgi apparatus proteomes in hepatocellular carcinomas and the surrounding liver tissues
    Yaying Yang, Yi Luo, Xiujun Li, Yongfen Yi
    Hepatology Research.2014; 44(5): 542.     CrossRef
  • Regulation of cell survival by the HIP-55 signaling network
    Chengzhi Yang, Zenggang Li, Zhi Shi, Kangmin He, Aiju Tian, Jimin Wu, Youyi Zhang, Zijian Li
    Molecular BioSystems.2014; 10(6): 1393.     CrossRef
  • Pro-oncogenic function of HIP-55/Drebrin-like (DBNL) through Ser269/Thr291-phospho-sensor motifs
    Zijian Li, Hae Ryon Park, Zhi Shi, Zenggang Li, Cau Dinh Pham, Yuhong Du, Fadlo R. Khuri, Youyi Zhang, Qide Han, Haian Fu
    Oncotarget.2014; 5(10): 3197.     CrossRef
  • Role-Shifting PKCζ Fosters Its Own Proapoptotic Destruction by Complexing with Bcl10 at the Nuclear Envelope of Human Cervical Carcinoma Cells: A Proteomic and Biochemical Study
    Anna Chiarini, Maddalena Marconi, Raffaella Pacchiana, Ilaria Dal Prà, Jun Wu, Ubaldo Armato
    Journal of Proteome Research.2012; 11(8): 3996.     CrossRef
  • Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration
    XIN LI, LONG CHEN, XIU JUN LIANG, YU FENG GAO, XIAO JIE WANG, QIAN XU, YONG YAN, FU LU GAO
    Molecular Medicine Reports.2012; 6(6): 1249.     CrossRef
  • Altered Carcinogenesis and Proteome in Mammary Glands of Rats after Prepubertal Exposures to the Hormonally Active Chemicals Bisphenol A and Genistein
    Angela M. Betancourt, Jun Wang, Sarah Jenkins, Jim Mobley, Jose Russo, Coral A. Lamartiniere
    The Journal of Nutrition.2012; 142(7): 1382S.     CrossRef
  • Modeling microRNA-mRNA Interactions Using PLS Regression in Human Colon Cancer
    Xiaohong Li, Ryan Gill, Nigel GF Cooper, Jae Keun Yoo, Susmita Datta
    BMC Medical Genomics.2011;[Epub]     CrossRef
  • α‐enolase: a promising therapeutic and diagnostic tumor target
    Michela Capello, Sammy Ferri‐Borgogno, Paola Cappello, Francesco Novelli
    The FEBS Journal.2011; 278(7): 1064.     CrossRef
  • Glucose‐regulated protein 58 modulates cell invasiveness and serves as a prognostic marker for cervical cancer
    Chia‐Jung Liao, Tzu‐I Wu, Ya‐Hui Huang, Ting‐Chang Chang, Chia‐Siu Wang, Ming‐Ming Tsai, Chyong‐Huey Lai, Ying Liang, Shih‐Ming Jung, Kwang‐Huei Lin
    Cancer Science.2011; 102(12): 2255.     CrossRef
  • Identification of Cervical Cancer Proteins Associated With Treatment With Paclitaxel and Cisplatin in Patients
    Huiling Liu, Yin Han, Ruoran Mi, Ying Zhang, Gang Su, Hailin Wang, Xin Zhou, Xiangwen Liu, Bingdong Zhu
    International Journal of Gynecological Cancer.2011; 21(8): 1452.     CrossRef
  • Use of cervicovaginal fluid for the identification of biomarkers for pathologies of the female genital tract
    Geert Zegels, Geert AA Van Raemdonck, Wiebren AA Tjalma, Xaveer WM Van Ostade
    Proteome Science.2010;[Epub]     CrossRef
  • Candidate Molecules Identified by Proteomic Study of Synovial Cells in Experimental Post-Traumatic Arthritis of Knee in Swine
    O. Baatartsog, K. Choi, P.K. Mandal, H.K. Lim, J.D. Oh, S.H. Chang
    Biotechnology(Faisalabad).2008; 8(1): 13.     CrossRef
  • 11,978 View
  • 67 Download
  • 17 Crossref
Close layer

Cancer Res Treat : Cancer Research and Treatment
Close layer
TOP