Cancer Research and Treatment

Kye-Hyun Nam

1 Article

Protein Expression Profile using Two-Dimensional Gel Analysis in Squamous Cervical Cancer Patients: Su-Mi Bae, Hyun-Jin Min, Guo Hua Ding, Sun-Young Kwak, Young-Lae Cho, Kye-Hyun Nam, Choong Hak Park, Yong-Wan Kim, Chong-Kook Kim, Byoung-Don Han, Young-Joo Lee, Do Kang Kim, Woong-Shick Ahn; Cancer Res Treat. 2006;38(2):99-107. Published online April 30, 2006; DOI: https://doi.org/10.4143/crt.2006.38.2.99

Purpose

Screening in cervical cancer is now progressing to discover candidate genes and proteins that may serve as biological markers and that play a role in tumor progression. We examined the protein expression patterns of the squamous cell carcinoma (SCC) tissues from Korean women with using two- dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight (MALDI- TOF) mass spectrometer.

Materials and Methods

Normal cervix and SCC tissues were solubilized and 2-DE was performed using pH 3~10 linear IPG strips of 17 cm length. The protein expression was evaluated using PDQuest 2-D software™. The differentially expressed protein spots were identified with a MALDI-TOF mass spectrometer, and the peptide mass spectra identifications were performed using the Mascot program and by searching the Swiss-prot or NCBInr databases.

Results

A total of 35 proteins were detected in SCC. 17 proteins were up-regulated and 18 proteins weredown-regulated. Among the proteins that were identified, 12 proteins (pigment epithelium derived factor, annexin A2 and A5, keratin 19 and 20, heat shock protein 27, smooth muscle protein 22 alpha, α-enolase, squamous cell carcinoma antigen 1 and 2, glutathione S-transferase and apolipoprotein a1) were protein previously known to be involved in tumor, and 21 proteins were newly identified in this study.

Conclusion

2-DE offers the total protein expression profiles of SCC tissues; further characterization of these differentially expressed proteins will give a chance to identify the badly needed tumor-specific diagnostic markers for SCC.

Citations

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