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Anti-tumor Effect of the Complex of Acriflavine and Guanosine (AG60)
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Eun Kyung Hong, Hwan Mook Kim, Kyung Yung Lee, Young Shin Chung, Bo Im Yoo, Sang Geon Kim, E Tay Ahn, Young Bok Han
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J Korean Cancer Assoc. 1997;29(1):29-37.
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 Abstract
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- PURPOSE
The anti-tumor effect of the complex of acriflavine and guanosine (AG60) was investigated.
MATERIALS AND METHODS
In vitro cytotoxicity of AG60 was measured using SRB assay, and in vivo antitumor activity of AG60 was examined in CDF1 mice intraperitoneally inoculated with the P388 leukemic cells and in ICR mice inguinally implanted with S-180 cells. Tumor size and mean survival time were determined.
RESULTS
AG60 and acriflavine showed strong anti-tumor effect in vitro on lung cancer (A549), renal cancer (UO-31) and colon cancer (COLO205) cells. However, AG60 did not show the cytotoxicity against normal cell line, 3T3. The range of the IC50 of AG60 to the various tumor cell lines was 0.09 microgram/ml through 1.94 microgram/ml. The treatment of ascitic tumor bearing CDF1 mice with AG60 resulted in over 160% increases in the mean survival time. The most effective dose of AG60 was 30 mg/kg body weight in tumor implanted mice. In solid tumor bearing ICR mice tumor growth and progression were suppressed in response to the different doses at 30 days; 69.8% suppression of tumor size in response to acriflavine, 16.0% to guanosine, 87.7% to AG60 and 78.5% to doxorubicin. In addition, 35% increases were observed in the means survival time of AG60 treated group compared with control group.
CONCLUSION
The prominant anti-tumor effects of AG60 shown in this report would represent the possibility of the clinical trials.
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Ultrastructural Study on the Secretory Duct of Parotid Gland of the Head - Irradiated Rat
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Seok Joon Yoo, Jin Gook Kim, Kyung Ho Park, Jeong Sik Ko, E Tay Ahn, Nam Gil Yang
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J Korean Cancer Assoc. 1994;26(2):311-322.
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Abstract
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- Function of salivary gland is severely disturbed during the radiotherapy for the patients with the head and neck cancers. Primary saliva is secreted by the glandula acini, but the various secretion from the ductal cells and the electrolyte control of ductal cells influence the component and character of the final saliva. In this study, the radiation effect on the duct cells of rat parotid gland was studied. Under sodium thiopental anesthesia, male rats weighing 200~250 g each were exposed to the radiation source of Mitshubishi linear accelerator(ML-4MV). Head and neck regions were exposured within the area of 30 30 cm, at the distance of 80 cm, in the depth of 1.2 cm, by the speed of 200 rads/min. Total doses were 3,000rads or 6,000 rads, and the survival times were 6 hours, 2 days or 6 days, respectively. Parotid glands were fixed in the 2.5% glutaraldehyde-l.5% paraformaldehyde solution, followed by refixation in the 1% osmic acid solution. Tissues were embedded within the araldite mixture. Thin sections were stained with the uranyl acetate-lead citrate solution. From the electron microscopic study, the following results were nbtained, l) Intercalated duct ce11s of the rat parotid gland were relatively organelle-poor, and they contained same secretory granules. Striated duct cells showed typical basal striations formed by the parallel infoldings together with the large number of mitochondria between the infoldings. Striated duct cell contained only a few secretory granules. 2) Following the irradiation, many intercalated duct cells showed atrophic change, thereafter content of secretory granule were increased, even without any structural recovery of the cyto- plasm. 3) The striated duct cells also showed the radiation effects. On the basal cytoplasm, the striations were disorganized or disappeared. But the content of secretory granules in the supranuclear region was largely increased. 4) Myoepithelial cells were altered or degenerated. It means that the autonomic control of ductal cell secretion is also distrubed. The cytology of the duct cells following the irradiation was interpreted as that, cessation or extreme slow-down of the secretory procedure was occurred by high dose irradiation. The electrolyte control function of the striated duct cell might be severely disturbed.
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KCA
