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The Expression of a Novel 90 kDa Stress Protein in Human Malignant Neoplasms
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Hong Rae Cho, Gyu Yeol Kim, Chan Jin Park, Byung Kyun Ko, Chang Woo Nam, Sung Sook Kim, Hae Who Park, Do Ha Kim, Sung Ryul Kim, Jeong Woo Park, Won Joon Yoon, Jeong Min Park, Seung Ju Cha, Wha Ja Cho, Dae Hwa Choi
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J Korean Cancer Assoc. 1999;31(4):793-801.
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 Abstract
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- PURPOSE
When cells are subjected to stressful stimuli such as, heat shock, toxic metal, nutrient deprivation, and metabolic disruption, they increase production of specific stress proteins that buffer them from harm. We reported that the expression of a navel 90 kDa cellular protein was increased by the infection of a fish rhabdovirus and heat shock in a fish cell. This new 90 kDa protein is not expressed in normal animal tissues but is highly induced in progressively transforming tissues or cells. That gives us some ideas tl at it is possible for this stress protein to be expressed in specific human cancer tissues.
MATERIALS AND METHODS
Commercialized checkerboard multi-tumor block (DAKO Co. Carpinteria, CA) was used for immunohistochemical analysis. The samples of human gastric cancer, colon cancer and breast cancer tissues were evaluated by Western blot and Northern blot for overexpression of the novel 90 kDa stress protein. Sera of those patients were analyzed by ELISA for the presence of antibody against the novel 90 kDa stress protein.
RESULTS
Immunohistochemical staining of human tumor tissue blocks showed significant immunostaining of novel 90 kDa stress protein in carcinomas such as colon cancer, breast cancer and stomach cancer but no apparent immunostaining in sarcomas. Coinciding with the immunohistochemical result, Western blotting and Northern blotting analyses indicate that the expression of the novel 90 kDa stress protein was increased in carcinomas. In addition, the antibody titer against the novel 90 kDa stress protein was found to be elevated in the sera of cancer patients.
CONCLUSIONS
The novel 90 kDa stress protein gene expression was elevated in carcinomas such as gastric cancer, breast cancer and colon cancer. These findings suggest that this new stress protein can be used as a tumor marker and may function as a chaperone in tumor growth.
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Correlation of Radiologic and Pathologic Lymph Non Involvement with TIMP-2 ( tissue inhibitors of metalloproteinase-2 ) in Gastric and Colon Carcinomas
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Soo Youn Ham, Jong Hwa Lee, Byung Kyun Ko, Hong Rae Cho, Dae Wha Choi, Chang Woo Nam, Sung Sook Kim, Woon Sup Han, Min Young Kim
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J Korean Cancer Assoc. 1999;31(1):9-15.
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Abstract
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- PURPOSE
To evaluate the correlation between the expression of TIMP-2 (tissue inhibitors of metalloproteinase-2) and negative lymph node involvement of colon and gastric carcinoma.
MATERIALS AND METHODS
We studied 26 cases (13 each) of gastric and colon carcinoma specimens along with dissected lymph nodes by immunohistochemical staining to investigate the correlation of the expression of TIMP-2. Lymph nodes involvement by CT scan was defined by size criteria and the presence of central low density.
RESULTS
Eight cases with positive lymph node involvement by CT scan showed weak expression of TIMP-2. Ten cases with positive lymph node involvement had weak expression of TIMP-2. Among eight cases with strong expression of TIMP-2 7 patients were negative by radiologic exam. Good correlation between strong TIMP-2 expression and negative lymph node involvement by CT scan was found (<0.05).
CONCLUSION
As the expression of TIMP-2 had a good correlation with radiologic involvement of lymph nodes, the study of expression of TIMP-2 in patients with stomach and colon carcinoma might be helpful in planning surgery and predicting the prognosis.
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KCA
